Impact of the colistin resistance gene mcr-1 on bacterial fitness

Tietgen, Manuela; Semmler, Torsten; Riedel-Christ, Sara; Kempf, Volkhard A. J.; Molinaro, Antonio; Ewers, Christa; Göttig, Stephan

doi:10.1016/j.ijantimicag.2017.11.011

Cited by 54 publications

(53 citation statements)

References 27 publications

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“…Pertinent examples where little or no fitness cost has been observed experimentally ( in vitro and/or in vivo ) in resistant organisms include: FQ‐resistant Campylobacter (Zhang, Lin, & Pereira, ), FQ‐resistant human E. coli (de Lastours et al., ), colistin‐resistant Salmonella (Rhouma et al., ), and E. coli after introduction of a MDR plasmid (Enne et al., ). Resistance plasmids appear in some cases to carry elements that can compensate for the cost of resistance they encode, as in the case of a colistin‐resistance ( mcr‐1 ) plasmid in E. coli (Tietgen et al., ). Assessment of E. coli strains bearing ESBL‐encoding plasmids did not identify a consistent fitness cost to the plasmid host in terms of growth or metabolic activity compared with plasmid‐cured variants, and indeed some of the plasmid‐bearing wild‐type strains had superior biofilming capability, which may be important in environmental survival (Schaufler et al., ).…”

Section: Control and Elimination Of Amr Organismsmentioning

confidence: 99%

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Davies

Wales

2019

Comp Rev Food Sci Food Safe

124

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Resistance to therapeutic antimicrobial agents is recognized as a growing problem for both human and veterinary medicine, and the need to address the issue in both of these linked domains is a current priority in public policy. Efforts to limit antimicrobial resistance (AMR) on farms have so far focused on control of the supply and use of antimicrobial drugs, plus husbandry measures to reduce infectious disease. In the United Kingdom and some other countries, substantial progress has been made recently against targets on agricultural antimicrobial drug use. However, evidence suggests that resistant pathogenic and commensal bacteria can persist and spread within and between premises despite declining or zero antimicrobial drug use. Reasons for this are likely complex and varied but may include: bacterial adaptations to ameliorate fitness costs associated with maintenance and replication of resistance genes and associated proteins, horizontal transmission of genetic resistance determinants between bacteria, physical transfer of bacteria via movement (of animals, workers, and equipment), ineffective cleaning and disinfection, and co‐selection of resistance to certain drugs by use of other antimicrobials, heavy metals, or biocides. Areas of particular concern for public health include extended‐spectrum cephalosporinases and fluoroquinolone resistance among Enterobacteriaceae, livestock‐associated methicillin‐resistant Staphylococcus aureus, and the emergence of transmissible colistin resistance. Aspects of biosecurity have repeatedly been identified as risk factors for the presence of AMR on farm premises, but there are large gaps in our understanding of the most important risk factors and the most effective interventions. The present review aims to summarize the present state of knowledge in this area, from a European perspective.

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Section: Control and Elimination Of Amr Organismsmentioning

confidence: 99%

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Davies

Wales

2019

Comp Rev Food Sci Food Safe

124

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“…Finally, we evaluated the contribution of YrInv and YrIlm to Y. ruckeri virulence using the Galleria mellonella (larvae of the greater wax moth) infection model, which has extensively been used to analyse pathogenicity of Gram‐negative bacteria (Tsai et al ., 2016; Tietgen et al ., 2018). G. mellonella larvae were injected with serially diluted bacterial suspensions of the NVH_3758 strain, and the median lethal dose that killed 50% (LD 50 ) of the larvae was calculated by monitoring the larvae survival over time (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Hence, we grew Yersinia at 37°C (Champion et al ., 2009). All Y. ruckeri strains showed a time and dosedependent killing of larvae with a log 10 LD 50 of approximately 10 5 , which is comparable to other important pathogens like Escherichia coli , Klebsiella pneumoniae or Acinetobacter baumannii (Tietgen et al ., 2018; Weidensdorfer et al ., 2019) thereby underlining the pathogenicity of Y. ruckeri . Deletion of yrInv or yrIlm or both reduced virulence by 50% compared to NVH_3758, which indicates that both IATs contribute modestly to virulence without showing a synergy in this infection model.…”

Section: Discussionmentioning

confidence: 99%

The inverse autotransporters of Yersinia ruckeri, YrInv and YrIlm, contribute to biofilm formation and virulence

Wróbel

Saragliadis

Pérez-Ortega

et al. 2020

Environmental Microbiology

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Summary Yersinia ruckeri causes enteric redmouth disease (ERM) that mainly affects salmonid fishes and leads to significant economic losses in the aquaculture industry. An increasing number of outbreaks and the lack of effective vaccines against some serotypes necessitates novel measures to control ERM. Importantly, Y. ruckeri survives in the environment for long periods, presumably by forming biofilms. How the pathogen forms biofilms and which molecular factors are involved in this process, remains unclear. Yersinia ruckeri produces two surface‐exposed adhesins, belonging to the inverse autotransporters (IATs), called Y. ruckeri invasin (YrInv) and Y. ruckeri invasin‐like molecule (YrIlm). Here, we investigated whether YrInv and YrIlm play a role in biofilm formation and virulence. Functional assays revealed that YrInv and YrIlm promote biofilm formation on different abiotic substrates. Confocal microscopy revealed that they are involved in microcolony interaction and formation, respectively. The effect of both IATs on biofilm formation correlated with the presence of different biopolymers in the biofilm matrix, including extracellular DNA, RNA and proteins. Moreover, YrInv and YrIlm contributed to virulence in the Galleria mellonella infection model. Taken together, we propose that both IATs are possible targets for the development of novel diagnostic and preventative strategies to control ERM.

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“…The transconjugant obtained in vitro from E. coli 13-220 acquired resistance to colistin, tetracycline, sulfamethoxazole and trimethoprim. Different studies have shown that the in vitro acquisition of an mcr-1-containing plasmid does not lead to a considerable fitness cost in E. coli as measured by growth kinetics, cytotoxicity, and virulence in a Galleria mellonella model (Tietgen et al, 2018;Zhang et al, 2017), but a high level of mcr-1 expression compromises growth rate, fitness, and the membrane's structural integrity while increasing cellular death (Yang et al, 2017). In our experiment, most isolates from samples collected from the E. coli 13-220 M-inoculated pigs shared the characteristics of this strain, suggesting that the commensal strains which had acquired the mcr-1 gene were perhaps not as competitive as E. coli 13-220 M.…”

Section: Discussionconclusionmentioning

confidence: 99%

Development of a pig infection model with colistin-resistant Escherichia coli

Devendec

Jouy

Paboeuf

et al. 2018

Veterinary Microbiology

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A B S T R A C TColistin-resistant Escherichia coli are isolated from pigs suffering from post-weaning diarrhea (PWD). This study was designed to develop an experimental model of PWD using mcr-1-carrying shiga toxin-producing E. coli (STEC) or enterotoxigenic E. coli (ETEC), for the future evaluation of control measures. Three groups of eight piglets, kept in high biosecurity units, were orally inoculated with mcr-1-positive STEC or ETEC, and one unchallenged group was used as a control. Clinical signs were recorded. Regularly-collected fecal samples and samples obtained from the digestive tract of animals sacrificed one month after inoculation were cultured in selective media and isolates were characterized. Blood samples were used to genotype the polymorphisms of the pigs' intestinal receptors for F4 and F18 E. coli adhesins.Diarrhea was more frequent and more fecal samples contained the inoculated strain in the group inoculated with the O149-F4 ETEC strain than with the O141-F18 or O139-F18 STEC strains. However, fewer positive samples were obtained from the two pigs with the F4 resistant genotype. The three inoculated strains could be re-isolated up to the end of the experiment. Excretion peaked on the first week after inoculation with the O149-F4 ETEC strain, and later for the other two. An mcr-1 gene transfer to other commensal isolates was observed only for O139-F18 STEC, while the loss of mcr-1 from the inoculated strain occurred in all groups. The O149-F4 ETEC challenge may be used to evaluate alternative solutions to combat PWD caused by colistin-resistant E. coli in pigs. (I. Kempf). Veterinary Microbiology 226 (2018) 81-88 0378-1135/

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Impact of the colistin resistance gene mcr-1 on bacterial fitness

Cited by 54 publications

References 27 publications

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

Antimicrobial Resistance on Farms: A Review Including Biosecurity and the Potential Role of Disinfectants in Resistance Selection

The inverse autotransporters of Yersinia ruckeri, YrInv and YrIlm, contribute to biofilm formation and virulence

Development of a pig infection model with colistin-resistant Escherichia coli

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