Impact of the Priobiotic on the Presence of Selected Virulence Genes and Drug-Resistance Among Campylobacter Coli Isolated from Piglets

Abstract

The investigations comprised 100 piglets of crossbreed Polish Landrace x Large White Polish breed. Faeces samples were collected on the 2 nd d of piglets' life (control). On the 5 th d of life of the piglets, probiotic paste was applied and 7 d later, faecal samples were collected again. The material included 100 isolates of Campylobacter sp. obtained from healthy piglets. All isolates were assigned to the Campylobacter coli species. The occurrence of virulence genes was determined by the PCR method. Drugresistance of the obtained isolates was determined using diffusion tests and E-test strips. All isolates deriving from the control group piglets were found to contain the cadF gene responsible for adhesion, as well as, gene flaA influencing motility of the examined bacteria. In piglets fed diets supplemented with probiotics, the cadF gene occurred in 100% isolates and gene flaA -in 99% isolates. Campylobacter coli isolates obtained from piglets from the control group exhibited the highest resistance with respect to ciprofloxacin and enrofloxacin. The similar results were recorded in the case of isolates obtained after the probiotic application. The majority of the isolates generated type haemolysis (91%-92%). No significant differences were recorded in the capability of generating haemolysis between isolates obtained before probiotic administration and the isolates obtained after the application of the experimental probiotic.

The prevalence of Campylobacter spp. and occurrence of virulence genes isolated from dogs

“…Biswas et al (2011) confirmed the occurrence of this gene in 100% of the examined C. jejuni strains derived from human clinical studies and cow faeces. Selwet and Galbas (2012a;2012b) observed that the cadF and flaA genes were found in 100% of C. coli and C. jejuni isolated from broilers, porkers, calves and piglets. All the strains of C. jejuni, C. coli C. upsaliensis under analysis carried the cdtB gene encoding the protein exhibiting toxic properties, i.e.…”

Monitoring of Virulence Genes, Drug-Resistance in Campylobacter coli Isolated from Golden Retrievers

“…According to the investigations by Andrzejewska et al (2011) and Selwet and Galbas (2012a;2012b) on C. coli occurrence in people, dogs, cats, and piglets, the presence of the flaA gene in all the examined isolates was also recorded (Selwet et al, 2015). The next factor affecting virulence is gene cadF, which is responsible for production of adhesines.…”

Monitoring wybranych genów zjadliwości u izolatów Campylobacter spp. pozyskanych od koni

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