Impact of the surface modifications and cell culture techniques on the biomechanical properties of PDMS in relation to cell growth behavior

Evaluation of the Cell Behavior and Growth Characteristics of the Porcine Dermal Xenograft Patch in Relation to the Surface Properties

“…After 48 h of incubation within the cell media, the porous surface of the patch was flattened compared to the untreated patch. Additionally, potassium chloride (KCl) and sodium chloride (NaCl) salts have been observed on the surface as bright regions ( Figure 1C ) as we described previously as well ( Aydemir et al, 2021 ) . The patch was incubated with 293T cells for 48 h; however, we did not observe cell clusters attached to the patch; controversially, a few cells could attach and grow on the patch after 48 h ( Figure 1D ).…”

“…Therefore, the dermal patch was incubated within cell media and cell media with cells for 48 h, respectively. SEM analysis showed that the surface of the dermal patch was flattened when set within cell media, and salt particles were formed on the patch ( Figures 1B–D ), as we reported previously ( Aydemir et al, 2021 ). On the other hand, cells could not attach and grow as clusters on the dermal patch, proven by the SEM pictures ( Figure 1D ).…”

“…Our data showed that indicated mineral levels in the cell media in which cells expanded did not significantly change compared to the cell media within cells growing on the dermal patch ( Figure 2 ). There was only a slight decrease in the cells growing on the patch, but that may have resulted from the formation of mineral salts on the patch observed via SEM ( Figure 2B ), as we have reported previously on PDMS biomaterials as well ( Aydemir et al, 2021 ) . Moreover, we investigated trace element levels in the cells.…”

“…On the other hand, orthobiologics should enable cells to distribute homogeneously on the patch and supply effective nutrient transport ( Spolarics and Navarro, 1994 ; Nauta et al, 2011 ). Mineral levels including Na, Ca, Mg, and K in the cell media of each group were measured to evaluate the mineral metabolism of the cells ( Aydemir et al, 2019a ; 2020b ; 2020a , 2021 ). Our data showed that indicated mineral levels in the cell media in which cells expanded did not significantly change compared to the cell media within cells growing on the dermal patch ( Figure 2 ).…”

“…Fe is required for cell cycle progression and cell proliferation; our data showed cells growing on the patch consumed less Fe compared to the control cells ( Figure 2 ). ( Aydemir et al, 2019a ; 2020b ; 2020a , 2021 ).…”