Impact of the Z potential technique on reducing the sperm DNA fragmentation index,
            fertilization rate and embryo development

Duarte, Carlos M.; Nunez,; Wong, Y. F.; Vivar, Carlos Alberto; Benites, Elder Omar; U, Rodriguez; Vergara, Carlos Alfredo; Ponce, Jordi

doi:10.5935/1518-0557.20170055

Cited by 10 publications

(10 citation statements)

References 25 publications

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“…However, in patients of group III where the zeta method was used for sperm selection, despite a high level of sperm DFI, the implantation rate improved. These results may be compared with other research studies in this field [17,31].…”

Section: Discussionmentioning

confidence: 69%

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Role of the Zeta Method in Intracytoplasmic Sperm Injection Outcomes in High Sperm DNA Fragmentation in Oligoasthenozoospermic Men

SefidgarTehrani

Amirian

Jalali

et al. 2018

GMJ

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Background: New methods are needed to optimize intracytoplasmic sperm injection (ICSI) outcomes in oligoasthenozoospermic (OAS) men. We evaluated the level of DNA fragment index (DFI) in OAS men and its impact on ICSI outcomes. In addition, we used the zeta potential method for sperm selection to investigate the efficacy of this technique in improving ICSI outcomes. Materials and Methods: This cross-sectional study was performed on 95 couples. Sperm parameters and sperm DNA fragmentation (SDF) were measured. The couples were divided into the following 3 groups: group I (n=30) where SDF was between 15% and 30%, and routine sperm was selected on the basis of motility and morphology; group II (n=34) where SDF was more than 30%, and the routine sperm selection method was applied on the basis of motility and morphology; and group III (n=31) where SDF was more than 30%, and the sperm selection was performed on the basis of the zeta method. The fertilization rate, embryo development, embryo quality, and implantation rate were evaluated in these 3 groups. Results: The fertilization rate was significantly higher in group I compared with group II (P<0.05). The embryo development rate in group I was significantly higher than that in group II (P<0.001) and group III (P<0.05), and it was significantly lower in group II compared with group III (P<0.05). The embryo quality was higher in group III compared with group II (P<0.01). The implantation rate in group I was significantly higher than that in group II (P<0.05) and group III (P<0.05). Conclusion: The present study indicated that a higher level of SDF has an adverse effect on the ICSI outcome. Furthermore, the zeta potential technique can be a useful method for sperm selection in OAS men. [GMJ.2018;7:e1107]

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Section: Discussionmentioning

confidence: 69%

“…Studies have shown that the selected sperm based on zeta capacity indicates a low level of DNA damage [16,17,31]. Esfahani et al [17] showed that the DGC/zeta method ameliorates the percentage of good-quality embryos and pregnancy rates compared with the DGC technique.…”

Section: Discussionmentioning

confidence: 99%

Role of the Zeta Method in Intracytoplasmic Sperm Injection Outcomes in High Sperm DNA Fragmentation in Oligoasthenozoospermic Men

SefidgarTehrani

Amirian

Jalali

et al. 2018

GMJ

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“…In our experience, by monitoring the presence of reacted sperm throughout the selection process, we were able to find a high percentage of unreacted sperm (as shown in the results section). The 95.35±1.18% of the spermatozoa not bound to the PNIPAM co-15% APTA surface showed an intact membrane, and, in the released spermatozoa, this evaluated parameter was 98.5±0.65% [39,40]. The results indicate that sperm selected by PNIPAM co-15%APTA surfaces are suitable for use in assisted reproduction techniques.…”

Section: Discussionmentioning

confidence: 88%

Development of Poly-NIsopropylacrylamide Surfaces for the Selection of Swine Sperm

Rodriguez¹

2021

Corpus J Vet Dairy Sci

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The reproductive efficiency of pig farms is directly correlated with the fertility of the boars. The aim of this work was to develop polymeric materials that can be used as a platform to select a subpopulation of sperm with better cell physiological parameters. Polymeric hydrogels composed of Poly-N-isopropylacrylamide with different positive charges given by copolymerization with (3-acrylamidopropyl) trimethylammonium chloride (APTA, 5-10-15%), were synthesized. Subsequently, the interaction between the sperm cells and the polymeric surfaces was analyzed in TALP medium. Release of the spermatozoa from the polymeric surfaces was induced by changing to Ca2+ free media. Sperm motility, cell viability, plasma membrane and acrosome integrity were evaluated. The results indicated that a higher percentage of swine sperm attached to PNIPAM co-15% APTA hydrogels (62.86±3.33%). Ninety seven percent (97.19±1.45 %) of the sperm released from the PNIPAM co-15%APTA surfaces were viable (p<0.05 vs unbound population and raw semen), with acceptable motility (58.89±1.28%) and with intact plasma and acrosomal membranes (69±1.2% and 98.5±0.65% respectively). These results indicate that hydrogels can be used to select boar sperm with high viability and mobility for use in assisted reproductive techniques.

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“…Other studies evaluated the efficiency of methods based on sperm electric charge in selecting DNA fragmentation-free spermatozoa. A significant reduction of SDF through a Zeta potential selection on spermatozoa recovered by DGC compared to DGC alone has been recently reported [145]. An electrophoresis method developed to select highquality human spermatozoa on the basis of their high negative charge [146] was shown to efficiently select spermatozoa without oxidative DNA damage in contrast to Percoll DGC that increase such damage compared to neat semen [147].…”

Section: Art Procedures That Generate Sperm Oxidative Stressmentioning

confidence: 99%

Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development

et al. 2021

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Reactive oxygen species (ROS) generated at low levels during mitochondrial respiration have key roles in several signaling pathways. Oxidative stress (OS) arises when the generation of ROS exceeds the cell’s antioxidant scavenging ability and leads to cell damage. Physiological ROS production in spermatozoa regulates essential functional characteristics such as motility, capacitation, acrosome reaction, hyperactivation, and sperm-oocyte fusion. OS can have detrimental effects on sperm function through lipid peroxidation, protein damage, and DNA strand breakage, which can eventually affect the fertility of an individual. Substantial evidence in the literature indicates that spermatozoa experiencing OS during in vitro manipulation procedures in human- and animal-assisted reproduction are increasingly associated with iatrogenic ROS production and eventual impairment of sperm function. Although a direct association between sperm OS and human assisted reproductive techniques (ART) outcomes after in vitro fertilization (IVF) and/or intracytoplasmic sperm injection (ICSI) is still a matter of debate, studies in animal models provide enough evidence on the adverse effects of sperm OS in vitro and defective fertilization and embryo development. This review summarized the literature on sperm OS in vitro, its effects on functional ability and embryo development, and the approaches that have been proposed to reduce iatrogenic sperm damage and altered embryonic development.

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Impact of the Z potential technique on reducing the sperm DNA fragmentation index, fertilization rate and embryo development

Cited by 10 publications

References 25 publications

Role of the Zeta Method in Intracytoplasmic Sperm Injection Outcomes in High Sperm DNA Fragmentation in Oligoasthenozoospermic Men

Role of the Zeta Method in Intracytoplasmic Sperm Injection Outcomes in High Sperm DNA Fragmentation in Oligoasthenozoospermic Men

Development of Poly-NIsopropylacrylamide Surfaces for the Selection of Swine Sperm

Sperm Oxidative Stress during In Vitro Manipulation and Its Effects on Sperm Function and Embryo Development

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