Impact of Therapeutic Treatment with β-Lactam on Transfer of the
 bla
 CTX-M-9
 Resistance Gene from
 Salmonella enterica
 Serovar Virchow to
 Escherichia coli
 in Gnotobiotic Rats

Faure, Stéphanie; Perrin-Guyomard, Agnès; Delmas, Jean-Michel; Laurentie, Michel

doi:10.1128/aem.00020-09

Cited by 11 publications

(12 citation statements)

References 39 publications

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“…In comparison to bla TEM and bla CMY genes, the prevalence of bla CTX-M gene in both E. coli and Salmonella from Meghalaya and Assam was found to be lower. Our result is also in corroboration with the report of other workers [ 13 - 15 ].…”

Section: Discussionsupporting

confidence: 94%

Extended-spectrum β-lactamases producing multidrug resistance Escherichia coli, Salmonella and Klebsiella pneumoniae in pig population of Assam and Meghalaya, India

Lalruatdiki¹,

Dutta²,

Roychoudhury³

et al. 2018

Vet World

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Aim:The present study was conducted to record the prevalence of extended spectrum β-lactamases (ESBLs) producing Escherichia coli, Salmonella spp., and Klebsiella pneumoniae from pig population of Assam and Meghalaya and to record the ability of the resistant bacteria to transfer the resistance genes horizontally.Materials and Methods:Fecal samples (n=228), collected from pigs of Assam (n=99) and Meghalaya (n=129), were processed for isolation and identification of E. coli and Salmonella spp. All the isolates were tested for ESBLs production by double disc synergy test (DDST) followed by screening for ESBLs producing genes (blaTEM, blaSHV, blaCTX-M, and blaCMY) by polymerase chain reaction (PCR). Possible transfer of resistance encoding genes between enteric bacterial species was carried out by in vitro and in vivo horizontal gene transfer (HGT) method.Results:A total of 897 enteric bacteria (867 E. coli and 30 Salmonella) were isolated and identified. Altogether 25.41% isolates were confirmed as ESBL producers by DDST method. Majority of the isolates were E. coli followed by Salmonella. By PCR, 9.03% isolates were found positive for at least one of the target resistance genes. blaSHV was absent in all the isolates. blaCMY was the most prevalent gene. All the E. coli isolates from Assam were negative for blaTEM. A total of 2.76% isolates were positive for blaTEM + blaCMY. On the other hand, 0.67% isolates were positive for blaCTX-M + blaCMY genes. Only 0.33% isolates carried all the three genes. Altogether, 4.68% bacteria carried the resistance encoding genes in their plasmids. blaTEM gene could be successfully transferred from Salmonella (donor) to E. coli (recipient) by in vitro (5.5-5.7×10−5) and in vivo (6.5×10−5 to 8.8×10−4) methods. In vivo method was more effective than in vitro in the transfer of resistance genes.Conclusion:The pig population of Assam and Meghalaya are carrying multidrug resistance and ESBLs producing E. coli and Salmonella. The isolates are also capable to transfer their resistance trait to other bacterial species by HGT. The present finding could be considered as a serious public health concern as similar trait can also be transmitted to the human commensal bacteria as well as pathogens.

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Section: Discussionsupporting

confidence: 94%

Extended-spectrum β-lactamases producing multidrug resistance Escherichia coli, Salmonella and Klebsiella pneumoniae in pig population of Assam and Meghalaya, India

Lalruatdiki¹,

Dutta²,

Roychoudhury³

et al. 2018

Vet World

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“…In other animal models, the intestinal microbiome can be a formidable barrier to the transfer of antimicrobial resistance (Schjørring et al, 2008;Faure et al, 2009;Stecher et al, 2012). However, this study showed that antimicrobial-resistant Salmonella occurred in the absence of administration of a resistant microbiome.…”

Section: Discussionmentioning

confidence: 66%

“…These plasmids often contain other resistances including chloramphenicol, florfenicol, streptomycin, and tetracycline (Winokur et al, 2000;Doublet et al, 2004;Fernández-Alarcón et al, 2011). In nature, Salmonella acquires MDR plasmids from commensals that inhabit the same environment (Winokur et al, 2001;Fricke et al, 2009;McCollister et al, 2016) and its acquisition of antimicrobial resistance is dependent on an abundant, antimicrobial-resistant donor population (Smith, 1975;Schjørring et al, 2008;Faure et al, 2009;Card et al, 2017). How quickly Salmonella acquires plasmids and associated antimicrobial resistances are dependent on the presence and abundance of the plasmid in the intestinal microbiome, rate of plasmid transmission, plasmid stability and fitness cost (Stewart and Levin, 1977;Ponciano et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistant Salmonella enterica Typhimurium Colonizing Chickens: The Impact of Plasmids, Genotype, Bacterial Communities, and Antibiotic Administration on Resistance

Bythwood

Soni

Lyons

et al. 2019

Front. Sustain. Food Syst.

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The rise in Salmonella resistance to cephalosporins and fluoroquinolones has become a significant threat to public health. At issue, is whether agricultural use of antimicrobials is selecting antibiotic resistance in Salmonella and the degree to which large antimicrobial resistance gene reservoirs, present in animal manures, contribute to this resistance. Two in vivo studies were performed to address these questions. In the first study, chickens were administered Salmonella and commensals, including an Escherichia coli strain with a mobile, ceftiofur-resistance plasmid, in order to determine how antibiotic administration impacted resistance in E. coli and Salmonella. All antibiotics administered to chickens increased streptomycin resistance in E. coli. However, only ceftiofur administration increased resistance in Salmonella and specifically to extended-spectrum β-lactams and cephalosporins (ESBL). There was no significant increase in ESBL-resistant Salmonella in chickens administered a ceftiofur-resistance plasmid donor. In the second study, chickens were administered two different isolates of S. enterica Typhimurium and a chicken resistome to serve as a gene donor. Birds were subsequently administered chlortetracycline or streptomycin. Antimicrobial administration significantly altered aminoglycoside and tetracycline resistance in the Enterobacteriaceae population. However, there was no significant increase in antimicrobial resistant Salmonella. Administration of a chicken resistome had no significant impact on prevalence of resistance in Enterobacteriaceae populations, including Salmonella. Evident, from both studies, was that these treatments had minimal effect on increasing the prevalence of resistance in Salmonella, suggesting that other factors may be more important in dissemination of antimicrobial resistant Salmonella in chickens.

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“…Therefore, the transfer of bacterial resistance genes of an animal origin to those of a human origin, and vice versa could be biologically plausible. At present, the evaluation and quantification of these genes flow between human and animal bacterial populations are not conducted [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Serogrouping and antibiotic resistance of Escherichia coli isolated from broiler chicken with colibacillosis in center of Algeria

Halfaoui¹,

Menoueri²,

Bendali³

2017

Vet World

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Aim:Colibacillosis is considered as one of the major bacterial infections in avian pathology. The excessive use of antibiotics reduced their effectiveness, which eventually led to the risk of emergence of antibiotic resistance. The aim of this study was to isolate, identify and serotype the pathogenic Escherichia coli strains and to determine their antibiotic susceptibility.Materials and Methods:A total of 180 samples from different organs of broilers with colibacillosis lesions were collected (liver, spleen, lung, and heart) in center of Algeria. The isolation and identification of E. coli were carried out using conventional techniques. Then, these strains were serotyped and tested over 13 antibiotics.Results:A total of 156 strains of E. coli were isolated. Serotyping results showed that 50 strains belong to 3 serotypes (23 for O1, 11 for O2, 16 for O78) which represent 32% of isolates. The antimicrobial susceptibility test, presented high level of resistance to tetracyclines (94.12%), flumequine (91.5%), sulfamethoxazole-trimethoprim (88.89%), enrofloxacin (86.27%), nalidixic acid (85.62%), ampicillin (83.01%) and doxycycline (75.81%), medium level resistance to chloramphenicol (39.22%), and amoxicillin-clavulanic acid (43.13%). All the strains were susceptible to cefotaxime, excepting three, which presented an extended spectrum β-lactamase (ESBL). In addition, the results of multi-resistance showed that all the strains were resistant at the minimum to two antibiotics and 66.66% of strains were resistant to at least seven antibiotics.Conclusion:The antibiotic resistance continues to rise at an alarming rate, and the emergence of ESBL is considered as a threat for public health.

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Impact of Therapeutic Treatment with β-Lactam on Transfer of the bla _CTX-M-9 Resistance Gene from Salmonella enterica Serovar Virchow to Escherichia coli in Gnotobiotic Rats

Cited by 11 publications

References 39 publications

Extended-spectrum β-lactamases producing multidrug resistance Escherichia coli, Salmonella and Klebsiella pneumoniae in pig population of Assam and Meghalaya, India

Extended-spectrum β-lactamases producing multidrug resistance Escherichia coli, Salmonella and Klebsiella pneumoniae in pig population of Assam and Meghalaya, India

Antimicrobial Resistant Salmonella enterica Typhimurium Colonizing Chickens: The Impact of Plasmids, Genotype, Bacterial Communities, and Antibiotic Administration on Resistance

Serogrouping and antibiotic resistance of Escherichia coli isolated from broiler chicken with colibacillosis in center of Algeria

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Impact of Therapeutic Treatment with β-Lactam on Transfer of the bla CTX-M-9 Resistance Gene from Salmonella enterica Serovar Virchow to Escherichia coli in Gnotobiotic Rats

Cited by 11 publications

References 39 publications

Extended-spectrum β-lactamases producing multidrug resistance Escherichia coli, Salmonella and Klebsiella pneumoniae in pig population of Assam and Meghalaya, India

Extended-spectrum β-lactamases producing multidrug resistance Escherichia coli, Salmonella and Klebsiella pneumoniae in pig population of Assam and Meghalaya, India

Antimicrobial Resistant Salmonella enterica Typhimurium Colonizing Chickens: The Impact of Plasmids, Genotype, Bacterial Communities, and Antibiotic Administration on Resistance

Serogrouping and antibiotic resistance of Escherichia coli isolated from broiler chicken with colibacillosis in center of Algeria

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Impact of Therapeutic Treatment with β-Lactam on Transfer of the bla _CTX-M-9 Resistance Gene from Salmonella enterica Serovar Virchow to Escherichia coli in Gnotobiotic Rats