Agroecosystem crop residues are considered to be a primary resource of organic substances, and are subject to the different transformation processes in the soil environment. The decomposition processes of organic substance can be also regulated by the application of the different biopreparations. The decomposition of organic substances in soil also causes changes in nitrogen content and its forms. It is essential for farmers to know about these processes in order flexibly to regulate and affect the transformation processes of the applied crop residues via the biopreparations directly in the production conditions. The changes of content and forms of nitrogen and its calculated parameters in Haplic Luvisol influenced by the applied crop residues (wheat – WR, rape – RR) and biopreparations (Betaliq, Trichomil) were monitored in the small-pot experiment carried out at the Department of Soil Science (FAFR, SUA-Nitra). The laboratory experiments were established for the incubation period 4, 7, 14, 28, 60, 90 and 180 days. The applied crop residues had statistically significant impact on the contents of total nitrogen (Nt) and potentially mineralizable nitrogen (Npot) and inorganic forms N, similarly also the values of labile nitrogen (LN) and nitrogen pool index (NPI). The biopreparations affected statistically significantly the average values Npot, LN, nitrogen lability index (NLI) and nitrogen management index (NMI). During the incubation period, the contents Npot and values LN and other calculated indexes of nitrogen fluctuated significantly.