Impact of toxicant exposure on the proteomic response to intertidal condition in Mytilus edulis

Impact of heat stress on Mytilus edulis: contaminants affect survival and HSP 70 proteoform expression

“…4 We confirmed this identification by mass spectrometry analysis (data not shown). Four spots were discriminated and for each one and each replicate, normalized volumes were calculated.…”

“…Protocol was adapted from Letendre et al 4 Briefly, gill proteins were extracted by Precellys grinding in 50 mM Tris buffer pH 7.8 containing urea 9M, CHAPS 2%, DTE 65 mM, 0.8% pharmalytes pH 3-10. The homogenate were sonicated 30 s in 3'' Cup-Horn (Maximum power: 100%, Qsonica) and centrifuged at 10,000 g, 20°C for 20 min.…”

The influence of zinc chloride and zinc oxide nanoparticles on air-time survival in freshwater mussels

“…In marine organisms, exposure to air for long periods leads to anoxic conditions and restricted food intake. Moreover, long emersion times of blue mussels leads to increased gene expression involved in energetic metabolism, protein chaperoning and maintenance of the cytoskeleton in cells (Letendre et al, 2011). They also showed that the presence of some contaminants could impact the same genes, which could have cumulative deleterious effects on mussels.…”

Proteomic changes in Corbicula fluminea exposed to wastewater from a psychiatric hospital

“…Briefly, peptides were enriched and desalted on a 40-nL RP-C18 trap column and separated on a Zorbax (30-nm pore size, 5-μm particle size) C18 column (43 mm long × 75 μm inner diameter; Agilent Technologies). A 9-min linear gradient (3-80 % acetonitrile in 0.1 % formic acid) at a flow rate of 400 nL/min was used, and the eluent was analysed with an Ion Trap mass spectrometer by using parameters previously described by Letendre et al (2011). Proteins were identified from the peptide mass fingerprint obtained by NanoLC-ESI-MS/MS using MASCOT (Matrix Science Inc, Boston, USA) or SpectrumMill softwares (Agilent Technologies) and 'PEAKS de novo Sequencing' method (Bioinformatics Solutions, Inc, Ontario, Canada).…”

“…Cell response to stress mainly consists in regulation of protein expression and metabolism (Letendre et al 2011). Therefore, proteomics constitute a powerful tool to understand the effects and modes of action (MoA) of contaminants in the aquatic environment (Manduzio et al, 2005, Tomanek and Zuzow 2010, Maria et al 2013.…”