Urban area expansion and the increase in the built-up area are major threats to agriculture, the natural environment, and ecology throughout the world. The population of the city of Larkana is rapidly increasing due to natural growth and uncontrolled migration from the surrounding areas, which have resulted in a haphazard increase in the built-up area over the fertile agricultural land and affected the city’s thermal environment. This research aims to evaluate the impacts of urban area expansion on agricultural land and the thermal environment of the study area by assessing the change in land use/ land cover (LULC) from 1990 to 2020 and land surface temperature (LST). For this purpose, the Random Forest (RF) algorithm was applied, and LST was calculated in Google Earth Engine (GEE) using Landsat imageries for the years 1990, 2000, 2010, and 2020. For the RF classifier algorithm, 3500 reference random points were generated for each year, which were then randomly divided into two datasets, i.e., a training sample consisting of 70% of the points and a validation dataset (30% of the points). After LULC classification, the results were validated for 1990, 2000, 2010, and 2020, and the accuracy was 88.3%, 89%, 90.01%, and 90.8%, respectively. The study results showed that the built-up area increased from 12.31 to 43.83 km2 while the barren land in the study region decreased from 56.51 km2 to 11.62 km2. The agricultural land was 66.66 km2 in 1990, expanded to 101.38 km2, and then decreased to 79.49 km2 in 2020. The results also revealed that most of the urban expansion in the last decade (2010-2020) took place on agricultural land. The urban thermal environment also showed a gradual increase in surface temperatur,e as recorded by the LST results. The LST results revealed that the maximum LST was 33.4°C in 1990, which increased to 36.1°C in 2020, and similarly, the minimum LST was 25.1°C, which also increased to 26.6°C in 2020. This study provides useful results for stakeholders to devise better policies and plans to control further haphazard urban expansion on fertile agricultural land in the study area.