Impact of UV-C irradiation on the quality, safety, and cytotoxicity of cranberry-flavored water using a novel continuous flow UV system

Gopisetty, Vybhav Vipul Sudhir; Patras, Ankit; Kilonzo‐Nthenge, Agnes; Yannam, Sudheer Kumar; Bansode, Rishipal R.; Sasges, Michael; Burns, Mallory; Pan, Che; Xiao, Hang

doi:10.1016/j.lwt.2018.04.042

Cited by 39 publications

(24 citation statements)

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“…Even though the effectiveness of UV-C light for the inactivation of microorganisms has been demonstrated, the treatment efficacy is dependent on the physical, optical and chemical properties of the liquid (Koutchma, 2009), impacting that efficacy of the treatment. Therefore, these factors must be optimized to establish adequate conditions for the process (Koutchma, 2009;Gopisetty et al, 2018). As A. vera is used in the formulation of food and pharmaceutical products (Domínguez-Fernández et al, 2012), it is important to preserve it without causing physicochemical changes that alter the quality and sensory characteristics.…”

Section: Introductionmentioning

confidence: 99%

MICROBIAL AND PHYSICOCHEMICAL PROPERTIES OF UV-C PROCESSED Aloe vera GEL BLENDS AT DIFFERENT pHs USING A CONTINUOUS FLOW UV SYSTEM

Rodríguez‐Rodríguez¹,

Quintero‐Ramos²,

Meléndez‐Pizarro³

et al. 2019

Rev.Mex.Ing.Quim.

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Section: Introductionmentioning

confidence: 99%

MICROBIAL AND PHYSICOCHEMICAL PROPERTIES OF UV-C PROCESSED Aloe vera GEL BLENDS AT DIFFERENT pHs USING A CONTINUOUS FLOW UV SYSTEM

Rodríguez‐Rodríguez¹,

Quintero‐Ramos²,

Meléndez‐Pizarro³

et al. 2019

Rev.Mex.Ing.Quim.

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“…UV irradiation has been demonstrated in literature as an effective physical method to inactivate chemical contaminants, and micro-organisms through photolysis and DNA damage, respectively [16][17][18][19][20] . This non-thermal technology is efficient in degrading aflatoxins because of their photosensitivity 21 .…”

mentioning

confidence: 99%

Performance of a UV-A LED system for degradation of aflatoxins B1 and M1 in pure water: kinetics and cytotoxicity study

Stanley

Patras

Pendyala

et al. 2020

Sci Rep

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The efficacy of a UV-A light emitting diode system (LED) to reduce the concentrations of aflatoxin B 1 , aflatoxin M 1 (AFB 1 , AfM 1) in pure water was studied. This work investigates and reveals the kinetics and main mechanism(s) responsible for the destruction of aflatoxins in pure water and assesses the cytotoxicity in liver hepatocellular cells. Irradiation experiments were conducted using an LED system operating at 365 nm (monochromatic wavelength). Known concentrations of aflatoxins were spiked in water and irradiated at UV-A doses ranging from 0 to 1,200 mJ/cm 2. The concentration of AFB 1 and AfM 1 was determined by HPLC with fluorescence detection. LC-MS/MS product ion scans were used to identify and semi-quantify degraded products of AFB 1 and AfM 1. It was observed that UV-A irradiation significantly reduced aflatoxins in pure water. In comparison to control, at dose of 1,200 mJ/cm 2 UV-A irradiation reduced AFB 1 and AfM 1 concentrations by 70 ± 0.27 and 84 ± 1.95%, respectively. We hypothesize that the formation of reactive species initiated by UV-A light may have caused photolysis of AFB 1 and AfM 1 molecules in water. In cell culture studies, our results demonstrated that the increase of UV-A dosage decreased the aflatoxins-induced cytotoxicity in HepG2 cells, and no significant aflatoxin-induced cytotoxicity was observed at UV-A dose of 1,200 mJ/cm 2. Further results from this study will be used to compare aflatoxins detoxification kinetics and mechanisms involved in liquid foods such as milk and vegetable oils. Filamentous fungi invading various feed crops produces toxic secondary metabolites called mycotoxins which possess a serious threat to consumer health 1. Aflatoxins are highly cytotoxic and carcinogenic secondary metabolites, produced predominantly by Aspergillus flavus and Aspergillus paraciticus, especially in the tropical and subtropical regions as hot and humid climatic conditions are optimal for mold growth and toxin production 2,3. Aflatoxins are difuranocoumarin derivatives formed from the polyketide pathway and include a group of 17 aflatoxins, among which AFB 1 is the predominant and is highly toxic 4. Aflatoxin contamination in animal feed results in their carry-over in foods of animal origin such as eggs and milk. The major AFB 1 metabolite in milk, AFM 1, is formed due to the action of hepatic cytochrome P450-dependent polysubstrate monoxygenase enzyme superfamily as a result of the hydroxylation of the fourth carbon of the terminal furan ring. In dairy cows, the biotransformation rate of AFB 1 to AFM 1 ranges from 0.3 to 6.2% 5,6. Aflatoxins are detrimental upon ingestion, inhalation, and skin contact; and the consequences of aflatoxin infection are collectively called aflatoxicosis. The biotransformation of AFB 1 in liver results in the production of AFB 1-8,9-epoxide which is highly related to the incidence of hepatocellular carcinoma 6,7. The carcinogenicity of AFM 1 is approximately one-tenth of that of AFB 1 The International Agency for Research on Cancer in 1993 classif...

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“…With regard to optical properties, it had an absorption coefficient of 0.483 ± 0.12 cm −1 , a penetration depth of 2.07 ± 0.03 cm, and a turbidity of 37.82 ± 0.41 NTU, indicating a relatively low content of suspended solids. These optical properties qualify the agave extract for its various applications via UV-C continuous flow technology [17]. The TP content was 6.17 ± 0.4 mg GAE/g d.w., which agreed with the data reported by Ahumada-Santos et al [8] for six different agave species, including Agave tequilana, and with the data reported by Santos-Zea et al [27] for concentrated agave sap.…”

Section: Physicochemical Characterization Of the Agave Extractmentioning

confidence: 84%

Effects of UV-C Irradiation and Thermal Processing on the Microbial and Physicochemical Properties of Agave tequilana Weber var. azul Extracts at Various pH Values

et al. 2020

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The effects of UV-C irradiation (at doses of 8.16, 10.93, 16.17, and 33.29 mJ/cm2) on the physicochemical and microbiological properties of Agave tequilana Weber extracts at various pH values (4.5, 5.5, and 6.5) were evaluated. Thermal treatment (TT) was used as a control (85 °C for 30 s). Both processed (UV-C or TT) and unprocessed (UP) extracts were investigated. The UV-C dose and the pH significantly (p < 0.05) affected the inactivation of total coliforms (TC), total aerobic mesophiles (TAM), and yeasts and molds (YM). UV-C doses of 10.93 mJ/cm2 at pH 4.5 and 33.29 mJ/cm2 at any agave extract pH completely inactivated the native microbial load compared to TT. The total polyphenols (TP), antioxidant activity (AA), and sugar content did not change in the agave extracts at any dose, but the total flavonoid (TF) content decreased at doses > 16.17 mJ/cm2 at the evaluated pH values. Although the color of the agave extracts (L*, a*, and b*) was significantly affected, the total color difference (∆E) did not change after processing compared to the ∆E in the UP extracts. TT further reduced all the physicochemical properties of the agave extracts compared to UV-C processing. The results suggest that UV-C continuous flow technology can be used to stabilize agave extracts at doses of 10.93 mJ/cm2 and pH 4.5, while preserving their functional properties.

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Impact of UV-C irradiation on the quality, safety, and cytotoxicity of cranberry-flavored water using a novel continuous flow UV system

Cited by 39 publications

References 39 publications

MICROBIAL AND PHYSICOCHEMICAL PROPERTIES OF UV-C PROCESSED Aloe vera GEL BLENDS AT DIFFERENT pHs USING A CONTINUOUS FLOW UV SYSTEM

MICROBIAL AND PHYSICOCHEMICAL PROPERTIES OF UV-C PROCESSED Aloe vera GEL BLENDS AT DIFFERENT pHs USING A CONTINUOUS FLOW UV SYSTEM

Performance of a UV-A LED system for degradation of aflatoxins B1 and M1 in pure water: kinetics and cytotoxicity study

Effects of UV-C Irradiation and Thermal Processing on the Microbial and Physicochemical Properties of Agave tequilana Weber var. azul Extracts at Various pH Values

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