Impact of Vitamin D on Expression of SIRT7 and CYP24A1 in Human Breast Cancer Cells

Abstract

Objectives: Impact of vitamin D (1, 25 dihydroxy vitamin D3) (calcitriol) in the regulation of different genes has been investigated in different cancers including breast cancer (BC). Material and Methods: In the current study, we analyzed the expression levels of the CYP24A1 and SIRT7 genes and their relationship with patients’ clinical data in BC using polymerase chain reaction (PCR). Afterward, we tend to analyze the effect of vitamin D on the expression of these genes in cell lines (MCF7 and MDAMB231) to find the regulatory role of vitamin D in BC. Results: Our results showed that the CYP24A1 and SIRT7 were increased by vitamin D treatment and CYP24A1 levels were related to tumors stages (p = 0.03) and up-regulation of CYP24A1, SIRT7 had the distinguish potential for breast cancer detection based on their receiver operative characteristic (ROC) curve results (0.77, 0.84, respectively). Conclusion: In summary, CYP24A1, SIRT7 may be used as a possible diagnostic biomarker in BC treatment.

Interleukin‐6 inhibitory effect of natural product naringenin compared to a synthesised monoclonal antibody against life‐threatening COVID‐19

“…However, there is still no specific treatment with any side effects available for COVID‐19 patients. Therefore, based on the increasing morbidity and mortality associated with COVID‐19, there is an urgent need to develop an efficient and safe therapeutic agent for the management of infected patients 15 . Naringenin (NAR) a new natural drug has been approved by clinical trials with its antiviral and anti‐inflammatory effects, availability, low cost, and long history.…”

“…Therefore, based on the increasing morbidity and mortality associated with COVID-19, there is an urgent need to develop an efficient and safe therapeutic agent for the management of infected patients. 15 Naringenin (NAR) a new natural drug has been approved by clinical trials with its antiviral and anti-inflammatory effects, availability, low cost, and long history. NAR has been pointed up in several in vivo studies with its anti-inflammatory potential, reducing viral replication and the production of cytokines.…”

The inhibitory role of stigmasterol on tumor growth by inducing apoptosis in Balb/c mouse with spontaneous breast tumor (SMMT)

“…Breast cancer (BC) has become one of the leading causes of death among women in the world; BC is considered the second deadliest cancer after lung cancer [ 1 , 2 ]. Currently, several treatments exist for BC patients, including surgery, radiation therapy, chemotherapy, gene therapy, and combination therapy [ 3 ].…”