Impact on Porphyromonas gingivalis of antimicrobial photodynamic therapy with blue light and Rose Bengal in plaque-disclosing solution

Yoshida, Ayaka; Inaba, Keitaro; Sasaki, Haruka; Hamada, Naoki; Yoshino, Fumihiko

doi:10.1016/j.pdpdt.2021.102576

Cited by 5 publications

(4 citation statements)

References 64 publications

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“…Numerous in vitro studies have shown that PDT can inhibit the growth of P. gingivalis and kill bacteria in infected cell models. 15,32,33 Some studies have indicated that PDT also significantly inhibits F. nucleatum or Aggregatibacter actinomycetemcomitans. 34,35 An in vivo study has found that PDT combined with supragingival scaling can reduce the amount of T. denticola and F. nucleatum in supragingival plaque at 30 and 60 days after treatment in orthodontic patients.…”

Section: Discussionmentioning

confidence: 99%

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Efficacy of photodynamic therapy as an adjunct to scaling and root planing on clinical parameters and microbial composition in subgingival plaque of periodontitis patients: A split‐mouth randomized clinical trial

Nie,

Huang,

Peng

et al. 2024

Journal of Periodontology

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BackgroundThe aim of this study was to assess the efficacy of photodynamic therapy (PDT) as an adjunct to scaling and root planing (SRP) on clinical parameters and microbial composition in subgingival plaque of periodontitis patients.MethodsSeventeen patients were included in this split‐mouth randomized clinical trial. Sites with probing pocket depth (PPD) ≥5 mm in combination with bleeding on probing in different quadrants were randomized into the control group, the group with a single PDT application right after SRP, and the group with three repeated PDT applications 1 week after SRP. The subgingival plaque was collected for 16S rRNA gene sequencing at baseline, Week 2, and Week 8.ResultsSeventeen patients with 60 sites completed this 8‐week follow‐up, and 157 subgingival plaques were successfully analyzed by sequencing. Significant improvements were observed in two primary outcomes: PPD at Week 8 and subgingival microbial composition. Compared to the control group, the repeated‐PDT group showed a notable improvement in PPD, substantial alterations in the microbial profile, including a reduction in α‐diversity and anaerobic bacteria, and an increase in aerobic bacteria at Week 2. Secondary outcomes, such as clinical attachment level and sulcus bleeding index, also showed improvement at Week 8. Furthermore, both the single‐ and repeated‐PDT groups exhibited a decrease in periodontopathogens and an increase in beneficial bacteria compared with baseline.ConclusionPDT promotes changes in the microbial composition of periodontitis patients’ subgingival plaque in a direction favorable to periodontal health, and repeated PDT is a promising adjunctive therapy for periodontal treatment.

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Section: Discussionmentioning

confidence: 99%

“…Numerous in vitro studies have shown that PDT can inhibit the growth of P. gingivalis and kill bacteria in infected cell models 15,32,33 . Some studies have indicated that PDT also significantly inhibits F. nucleatum or Aggregatibacter actinomycetemcomitans 34,35 .…”

Section: Discussionmentioning

confidence: 99%

Efficacy of photodynamic therapy as an adjunct to scaling and root planing on clinical parameters and microbial composition in subgingival plaque of periodontitis patients: A split‐mouth randomized clinical trial

Nie,

Huang,

Peng

et al. 2024

Journal of Periodontology

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“…Curcumin (maximum absorption wavelength: 425 nm), riboflavin (266 nm, 373 nm, 445 nm), rose bengal (550 nm), erythrosine (530 nm), and sinoporphyrin sodium (366 nm) have been used as photosensitizers in a-PDT using third-generation blue LED light [55]. The antibacterial effect of a-PDT in combination with blue LEDs and the aforementioned photosensitizers has been demonstrated in vitro on periodontal bacteria commonly found in patients with periodontitis, such as A. actinomycetemcomitans, Campylobacter rectus, Fusobacterium nucleatum, Parvimonas micra, P. gingivalis, Tannerella forsythia, Prevotella intermedia, and Streptococcus gordonii [56][57][58][59][60][61][62][63][64][65]. In addition, the antibacterial activity of blue light irradiation alone has been demonstrated against some oral bacteria (Figure 3) [36,[66][67][68][69][70][71], further MRSA, and novel coronavirus [72,73].…”

Section: Blue Lightmentioning

confidence: 99%

Application of Different Wavelengths of LED Lights in Antimicrobial Photodynamic Therapy for the Treatment of Periodontal Disease

Takeuchi,

Aoki,

Hiratsuka

et al. 2023

Antibiotics

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Therapeutic light has been increasingly used in clinical dentistry for surgical ablation, disinfection, bio-stimulation, reduction in inflammation, and promotion of wound healing. Photodynamic therapy (PDT), a type of phototherapy, has been used to selectively destroy tumor cells. Antimicrobial PDT (a-PDT) is used to inactivate causative bacteria in infectious oral diseases, such as periodontitis. Several studies have reported that this minimally invasive technique has favorable therapeutic outcomes with a low probability of adverse effects. PDT is based on the photochemical reaction between light, a photosensitizer, and oxygen, which affects its efficacy. Low-power lasers have been predominantly used in phototherapy for periodontal treatments, while light-emitting diodes (LEDs) have received considerable attention as a novel light source in recent years. LEDs can emit broad wavelengths of light, from infrared to ultraviolet, and the lower directivity of LED light appears to be suitable for plaque control over large and complex surfaces. In addition, LED devices are small, lightweight, and less expensive than lasers. Although limited evidence exists on LED-based a-PDT for periodontitis, a-PDT using red or blue LED light could be effective in attenuating bacteria associated with periodontal diseases. LEDs have the potential to provide a new direction for light therapy in periodontics.

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“…A considerable number of studies have been conducted on the effects of PDT on microorganisms involved with oral diseases, including Porphyromonas gingivalis ( P. gingivalis ; Ding et al, 2021 ; Ghazi et al, 2021 ; Oruba et al, 2021 ; Yoshida et al, 2021 ) as a gram-negative obligate anaerobe bacterium ( Hajishengallis et al, 2012 ) which is the main representative of pathogenic bacteria involved in periodontal diseases ( Ding et al, 2021 ), Streptococcus mutans ( S. mutans ; Benine-Warlet et al, 2022 ; Dos Santos et al, 2022 ; Fernandes et al, 2022 ) which is the main bacteria involved in caries development, and Candida albicans which is the main opportunistic fungus in the oral microbial flora ( de Lapena et al, 2022 ; Dos Santos et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Clinical applications of antimicrobial photodynamic therapy in dentistry

et al. 2023

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Given the emergence of resistant bacterial strains and novel microorganisms that globally threaten human life, moving toward new treatment modalities for microbial infections has become a priority more than ever. Antimicrobial photodynamic therapy (aPDT) has been introduced as a promising and non-invasive local and adjuvant treatment in several oral infectious diseases. Its efficacy for elimination of bacterial, fungal, and viral infections and key pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Candida albicans, and Enterococcus faecalis have been investigated by many invitro and clinical studies. Researchers have also investigated methods of increasing the efficacy of such treatment modalities by amazing developments in the production of natural, nano based, and targeted photosensitizers. As clinical studies have an important role in paving the way towards evidence-based applications in oral infection treatment by this method, the current review aimed to provide an overall view of potential clinical applications in this field and summarize the data of available randomized controlled clinical studies conducted on the applications of aPDT in dentistry and investigate its future horizons in the dental practice. Four databases including PubMed (Medline), Web of Science, Scopus and Embase were searched up to September 2022 to retrieve related clinical studies. There are several clinical studies reporting aPDT as an effective adjunctive treatment modality capable of reducing pathogenic bacterial loads in periodontal and peri-implant, and persistent endodontic infections. Clinical evidence also reveals a therapeutic potential for aPDT in prevention and reduction of cariogenic organisms and treatment of infections with fungal or viral origins, however, the number of randomized clinical studies in these groups are much less. Altogether, various photosensitizers have been used and it is still not possible to recommend specific irradiation parameters due to heterogenicity among studies. Reaching effective clinical protocols and parameters of this treatment is difficult and requires further high quality randomized controlled trials focusing on specific PS and irradiation parameters that have shown to have clinical efficacy and are able to reduce pathogenic bacterial loads with sufficient follow-up periods.

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Impact on Porphyromonas gingivalis of antimicrobial photodynamic therapy with blue light and Rose Bengal in plaque-disclosing solution

Cited by 5 publications

References 64 publications

Efficacy of photodynamic therapy as an adjunct to scaling and root planing on clinical parameters and microbial composition in subgingival plaque of periodontitis patients: A split‐mouth randomized clinical trial

Efficacy of photodynamic therapy as an adjunct to scaling and root planing on clinical parameters and microbial composition in subgingival plaque of periodontitis patients: A split‐mouth randomized clinical trial

Application of Different Wavelengths of LED Lights in Antimicrobial Photodynamic Therapy for the Treatment of Periodontal Disease

Clinical applications of antimicrobial photodynamic therapy in dentistry

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