Impacto del ozono troposférico sobre la anatomía foliar de Abies pinsapo Boiss. I.: Estudio de la distribución de daños

Abstract

RESUMEN. Impacto del ozono troposférico sobre la anatomía foliar de Abies pinsapo Boiss. I: Estudio de la distribución de daños. Con el fin de elaborar unos criterios unificados para la evaluación del impacto del ozono troposférico y compararlos con la respuesta de las poblaciones naturales de Sierra Bermeja y Sierra de las Nieves, se han reproducido en cámaras Open Top, ambientes filtrados y enriquecidos con ozono para realizar una caracterización microscópica del daño que produce el ozono troposférico sobre Abies pinsapo Boiss. Los resultados demuestran que pese a que la morfología foliar permanece inalterada tras someter durante un mes en condiciones de 30 ppb durante 8 horas al día, a nivel tisular se han producido daños de consideración que afectan de este endemismo.Palabras clave. Anatomía, Abies pinsapo, ozono, cámaras Open Top.ABSTRACT. Tropospheric ozone injury on the foliar anatomy of Abies pinsapo Boiss. I: Study of damage distribution. With the aim of develop unified criteria to evaluate the tropospheric ozone injury and compare with responses in Abies pinsapo Boiss. in natural populations in Sierra Bermeja and Sierra de las Nieves, it has been reproduced in Open Top Chambers conditions of filtered air and addition of controlled amounts of ozone. Results show that despite leaf morphology remains unaltered after a treatment with 30 ppb 8 hours per day, at tissue level there have been injuries that affect this endemism.Key words. Anatomy, Abies pinsapo, ozone, Open Top chambers.

Histologic, metabolomic, and transcriptomic differences in fir trees from a peri‐urban forest under chronic ozone exposure

“…Cell collapse was particularly important within the palisade parenchyma (Figure 2b , Appendix S2 ; Alvarez et al., 1998 ; Evans & Fitzgerald, 1993 ; Terrazas & Bernal‐Salazar, 2002 ), which has been attributed to oxidizing agents that act on the middle lamella of the cell wall and promote its degradation (Gimeno & Ibars, 2009 ). Such degradation increases intercellular spaces and leads to cell death (Alvarez et al., 1998 ), and it is often accompanied by the accumulation of phenolic and tannin compounds that produce the characteristic reddish coloration of O 3 damage (Figure 2b , Appendix S2 ; Gostin, 2010 ).…”

“…Afterward, they were mounted on slides and dried for 15 days in an oven at 56°C. We looked for cell structures previously reported as symptoms of O 3 damage (Appendix S2 ; Gimeno & Ibars, 2009 ). Samples were photographed in an Axioskope Car Zeiss photomicroscope for examining tissue‐level damage, compared to a reference description of A. religiosa (Alvarez et al., 1998 ).…”

Histological, metabolomic, and transcriptomic differences in fir trees from a peri-urban forest under chronic ozone exposure

“…Afterwards, they were mounted on slides and dried for 15 days in an oven at 56° C. We looked for cell structures previously reported as symptoms of O3 damage (Fig. S2; Gimeno & Ibars, 2009). Samples were photographed in an Axioskope Car Zeiss photomicroscope for examining tissue-level damage, compared to a reference description of A. religiosa (Alvarez et al, 1998).…”

“…Cell collapse was particularly important within the palisade parenchyma (Fig. 2b, S2; (Alvarez et al, 1998;Evans & Fitzgerald, 1993;Terrazas & Bernal-Salazar, 2002), which has been attributed to oxidizing agents that act on the middle lamella of the cell wall and promote its degradation (Gimeno & Ibars, 2009). Such degradation increases intercellular spaces and leads to cell death (Alvarez et al, 1998), and it is often accompanied by the accumulation of phenolic and tannin compounds that produce the characteristic reddish coloration of O3 damage (Fig.…”