Impacts of broth chemistry on silver ion release, surface chemistry composition, and bacterial cytotoxicity of silver nanoparticles

Johnston, Kathryn A.; Stabryla, Lisa M.; Smith, Ashley M.; Gan, Xing Yee; Gilbertson, Leanne M.; Millstone, Jill E.

doi:10.1039/c7en00974g

Cited by 22 publications

(38 citation statements)

References 45 publications

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Section: E Coli Develops Resistance To Agnps But Not To Ag(i) Ionsmentioning

confidence: 99%

“…Pseudospherical citrate-capped AgNPs were synthesized according to a previously reported procedure [20][21][22] and functionalized with poly(ethylene glycol) methyl ether thiol (PEGSH, average molecular weight 1,000 g mol −1 ) using mass action ligand exchange (Scheme S1 in ref. 20 ). Poly(ethylene glycol) methyl ether thiol is a widely used biocompatible and charge-neutral ligand 20,23 which mitigates electrostatic attractive forces with negatively charged bacteria.…”

Section: E Coli Develops Resistance To Agnps But Not To Ag(i) Ionsmentioning

confidence: 99%

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Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions

et al. 2021

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Unlike conventional antimicrobials, the study of bacterial resistance to silver nanoparticles (AgNPs) remains in its infancy and the mechanism(s) through which it evolves are limited and inconclusive. The central question remains whether bacterial resistance is driven by the AgNPs, released Ag(I) ions or a combination of these and other factors. Here, we show a specific resistance in an Escherichia coli K-12 MG1655 strain to subinhibitory concentrations of AgNPs, and not Ag(I) ions, as indicated by a statistically significant greater-than-twofold increase in the minimum inhibitory concentration occurring after eight repeated passages that was maintained after the AgNPs were removed and reintroduced. Whole-population genome sequencing identified a cusS mutation associated with the heritable resistance that possibly increased silver ion efflux. Finally, we rule out the effect of particle aggregation on resistance and suggest that the mechanism of resistance may be enhanced or mediated by flagellum-based motility.

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Section: E Coli Develops Resistance To Agnps But Not To Ag(i) Ionsmentioning

confidence: 99%

Section: E Coli Develops Resistance To Agnps But Not To Ag(i) Ionsmentioning

confidence: 99%

Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions

et al. 2021

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“…5). As a background, antibacterial tests with AgNPs and silver-releasing materials have previously been performed in different bacterial culture media 50,51 , phosphate buffer 3,16 , PBS buffer 18,33 , simple aquatic media (for example containing only NaCl and KCl 52 ), dechlorinated tap water 53 or deionized water 32 . However, in these cases, [Cl − ] is typically either very high or very low compared to freshwaters.…”

Section: Resultsmentioning

confidence: 99%

Effects of Chloride Concentration on the Water Disinfection Performance of Silver Containing Nanocellulose-based Composites

Lehtonen

Hassinen

Honkanen³

et al. 2019

Sci Rep

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The availability of microbially-safe drinking water is a challenge in many developing regions. Due to the well-known antibacterial effect of silver ions, materials used for their controlled release have been widely studied for point-of-use water disinfection. However, even if it is in principle known that chloride anions can suppress the antibacterial efficiency of silver, the majority of previous studies, surprisingly, have not focused on chloride concentrations relevant for freshwaters and thus for practical applications. Here, we prepared low-cost nanocellulose-aluminium oxyhydroxide nanocomposites functionalized with silver nanoparticles. Field samples obtained from Chennai, India were used as a guideline for choosing relevant chloride concentrations for the antibacterial studies, i.e., 10, 90, and 290 ppm. The antibacterial performance of the material against Escherichia coli and Bacillus subtilis was demonstrated and the influence of chloride concentration on the antibacterial effect was studied with E. coli. A 1 h contact time led to bacterial reductions of 5.6 log10, 2.9 log10, and 2.2 log10, respectively. This indicates that an increase of chloride concentration leads to a substantial reduction of antibacterial efficiency, even within chloride concentrations found in freshwaters. This work enables further insights for designing freshwater purification systems that utilize silver-releasing materials.

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“…Studies have reported that bacterial growth media could impact on ions bioavailability and therefore on cell's response and shape under stress condition [41][42][43] . We can suspect that, since culture media contains different chemical compounds as phosphates and chlorates, silver precipitation may occur distinctly depending on the medium composition.…”

Section: Effect Of Culture Media (Malate Medium Versus Lb Medium) On Ag + Toxicity In R Gelatinosusmentioning

confidence: 99%

“…More details analysis, trying to distinguish between several chemical compounds (phosphates, malic acid, carboxylates, chlorates, …) in broth composition 42,43,53,54 and also light effect, is actually carried in our group. Furthermore, these preliminary results are sufficient to establish a link between observed aggregates and silver precipitation process due to medium solution.…”

Section: Formation Of Ag + Aggregates In Malate/lb or Citratementioning

confidence: 99%

Revealing Silver Effects on Bacterial Cell Structure by Atomic Force and Scanning Electron Microscopies

Tambosi¹,

Djafari²,

Liotenberg³

et al. 2021

Preprint

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The use of silver (Ag+) as an antimicrobial under different forms and at different scales, appears in numerous applications such as in health care, food industry, clothing, fabrics and disinfectants. Yet, there is still important gaps regarding the complete comprehension of the mechanisms of its actions on bacteria. In a previous work, we demonstrated that, silver and copper severly damage membrane proteins involved in photosynthesis and respiration in bacteria exposed to metal excess. Here, we are presenting complementary data using AFM and SEM microscopies, that reveals (i) the drastic effects of Ag+ ions on the morphology and structure of cell membrane and (ii) the formation of Ag+ aggregates that adhere to the bacterial cell surface in Rubrivivax (R.) gelatinosus . Impacts of Ag+ ions on R. gelatinosus are compared to those on the most commonly studied bacteria (Escherichia (E.) coli and Bacillus (B.) subtilis ), while considering the effect of culture grown media on the modification of silver ions. Altogether, these results reveal other levels and subtle aspects of Ag+ toxicity to be taken into account in understanding the general mechanisms of metal toxicity in bacteria.

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Impacts of broth chemistry on silver ion release, surface chemistry composition, and bacterial cytotoxicity of silver nanoparticles

Abstract: We determine the impact of bacterial growth media on silver nanoparticle surface chemistry, this surface chemistry on silver ion release from the nanoparticles, and ultimately the antimicrobial implications of those parameters.

Cited by 22 publications

References 45 publications

Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions

Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions

Effects of Chloride Concentration on the Water Disinfection Performance of Silver Containing Nanocellulose-based Composites

Revealing Silver Effects on Bacterial Cell Structure by Atomic Force and Scanning Electron Microscopies

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