Impacts of Bt Rice Expressing Cry1C or Cry2A Protein on the Performance of Nontarget Leafhopper,<i>Nephotettix cincticeps</i>(Hemiptera: Cicadellidae), Under Laboratory and Field Conditions

Lu, Zengbin; Tian, Jun-Ce; Wang, W.; Xu, H. X.; Hu, Cui; Guo, Yong; Peng, Yu; Ye, G. Y.

doi:10.1603/en13160

Cited by 16 publications

(16 citation statements)

References 51 publications

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“…A series of investigations has been conducted to assess potential risks of transgenic Bt rice to non-target herbivores and their natural enemies, including parasitoids of pests in rice agroecosystems at population levels. For example, no adverse effects of Bt rice on fitness parameters (developmental duration, survival rate and fecundity) of non-target herbivorous insects, such as brown planthoppers (BPHs), leafhoppers, and soil collembolans, have been identified [9][10][11]. Similar results also were obtained in studies of fitness parameters of predatory Pardosa pseudoannulata, Ummeliata insecticeps, Cyrtorhinus lividipennis, Propylea japonoca and frogs [12][13][14][15][16].…”

Section: Introductionsupporting

confidence: 61%

Cry1Ab-expressing rice did not influence expression of fecundity-related genes in the wolf spider Pardosa pseudoannulata

Wang

Peng

et al. 2016

Gene

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The impact of Bacillus thuringiensis (Bt) toxin proteins on non-target predatory arthropods is not well understood at the cellular and molecular levels. Here, we investigated the potential effects of Cry1Ab expressing rice on fecundity of the wolf spider, Pardosa pseudoannulata, and some of the underlying molecular mechanisms. The results indicated that brown planthoppers (BPHs) reared on Cry1Ab-expressing rice accumulated the Cry toxin and that reproductive parameters (pre-oviposition period, post-oviposition stage, number of eggs, and egg hatching rate) of the spiders that consumed BPHs reared on Bt rice were not different from those that consumed BPHs reared on the non-Bt control rice. The accumulated Cry1Ab did not influence several vitellin (Vt) parameters, including stored energy and amino acid composition, during one generation. We considered the possibility that the Cry toxins exert their influence on beneficial predators via more subtle effects detectable at the molecular level in terms of gene expression. This led us to transcriptome analysis to detect differentially expressed genes in the ovaries of spiders exposed to dietary Cry1Ab and their counterpart control spiders. Eight genes, associated with vitellogenesis, vitellogenin receptor activity, and vitellin membrane formation were not differentially expressed between ovaries from the treated and control spiders, confirmed by qPCR analysis. We infer that dietary Cry1Ab expressing rice does not influence fecundity, nor expression levels of Vt-associated genes in P. pseudoannulata.

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Section: Introductionsupporting

confidence: 61%

Cry1Ab-expressing rice did not influence expression of fecundity-related genes in the wolf spider Pardosa pseudoannulata

Wang

Peng

et al. 2016

Gene

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“…In laboratory assessments, several GRLH ecological fitness parameters, specifically nymphal duration, adult longevity, and fecundity were influenced by Bt rice, which varied according to the expressed insecticidal proteins, Cry1Ab [ 21 ], Cry2A or Cry1C [ 22 ]. Similarly, field investigations indicated that two types of Bt rice expressing a fused protein, Cry1Ab/Cy1Ac [ 23 , 24 ], or a single protein, Cry1Ab [ 21 ] resulted in a higher GRLH population density compared to the controls.…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, field investigations indicated that two types of Bt rice expressing a fused protein, Cry1Ab/Cy1Ac [ 23 , 24 ], or a single protein, Cry1Ab [ 21 ] resulted in a higher GRLH population density compared to the controls. Other Bt rice lines expressing a fused Cry1Ab/Vip3H protein [ 25 ], or single Cry2A, or Cry1C proteins [ 22 ] showed no significant effects on population density.…”

Section: Introductionmentioning

confidence: 99%

Combined influence of Bt rice and rice dwarf virus on biological parameters of a non-target herbivore, Nephotettix cincticeps (Uhler) (Hemiptera: Cicadellidae)

et al. 2017

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The advent of genetically modified (GM) Bt rice creates the possibility of interactions among Bt crops, crop pathogens and non-target herbivores. In particular, information on how pathogen-infected Bt-expressing plants will influence non-target herbivores is necessary to predict the sustainability of GM cropping systems. Laboratory bioassays were conducted to evaluate the potential combined impacts of rice dwarf virus (RDV) and two Bt rice lines, T1C-19 (Cry1C) and T2A-1 (Cry2A), on non-target green rice leafhopper (GRLH), Nephotettix cincticeps (Uhler) (Hemiptera: Cicadellidae). In the first experiment, GRLHs feeding preference tests on Bt rice lines compared to a parental control rice line, MH63, were conducted. As rice plants were uninfected with RDV, GRLHs generally preferred the control MH63 line over the two Bt lines during the initial 8 h, with no significant preference during the following 64 h. As rice plants were infected with RDV, there were no clear preferences between the Bt rice lines and the control MH63 line. In the second experiment, we assessed the combined influence of RDV-infection status and Bt rice lines on GRLH biological parameters. Egg duration, adult weights, and male adult longevity were significantly affected on RDV-infected Bt rice. Other parameters, egg hatching rate, nymph survival and fecundity were not significantly influenced. We infer that interaction effect among two testing Bt rice lines and RDV will not lead to enlarged pest populations, thus demonstrating that growing these two Bt rice lines will poses negligible risk to GRLH in sustainable rice agroecosystems. Long-term field experiments to monitor the population dynamics of GRLHs at large scale need to be carried out to confirm the current results.

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“…In addition to pest natural enemies, other potential effects of Bt rice on nontarget herbivores also have been tested, focusing on planthoppers, leafhoppers and thrips. Laboratory studies showed that feeding on Bt rice producing Cry1Ac, Cry1Ab, Cry1C, Cry2A and vip3H proteins had no negative effect on survival and development of the planthoppers Nilaparvata lugens and Sogatella fucifera (Chen et al ., ; Fu et al ., ; Lu et al ., ,c, ; Mannakkara et al ., ; Zhang et al ., ). In accord with the results of laboratory studies, field trials did not find consistent differences in the population dynamics of planthoppers and thrips between Bt rice and nonrice fields (Akhtar et al ., ,b; Chen et al ., , ; Jiao et al ., ; Li et al ., ; Liu et al ., ,b; Romeis et al ., ;)…”

Section: Issues Facing Adoption Of Bt Rice In Chinamentioning

confidence: 99%

The development and status of Bt rice in China

Hallerman

Liu

et al. 2015

Plant Biotechnology Journal

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SummaryMultiple lines of transgenic rice expressing insecticidal genes from the bacterium Bacillus thuringiensis (Bt) have been developed in China, posing the prospect of increases in production with decreased application of pesticides. We explore the issues facing adoption of Bt rice for commercial production in China. A body of safety assessment work on Bt rice has shown that Bt rice poses a negligible risk to the environment and that Bt rice products are as safe as non-Bt control rice products as food. China has a relatively well-developed regulatory system for risk assessment and management of genetically modified (GM) plants; however, decision-making regarding approval of commercial production has become politicized, and two Bt rice lines that otherwise were ready have not been allowed to enter the Chinese agricultural system. We predict that Chinese farmers would value the prospect of increased yield with decreased use of pesticide and would readily adopt production of Bt rice. That Bt rice lines may not be commercialized in the near future we attribute to social pressures, largely due to the low level of understanding and acceptance of GM crops by Chinese consumers. Hence, enhancing communication of GM crop science-related issues to the public is an important, unmet need. While the dynamics of each issue are particular to China, they typify those in many countries where adoption of GM crops has been not been rapid; hence, the assessment of these dynamics might inform resolution of these issues in other countries.

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Impacts of Bt Rice Expressing Cry1C or Cry2A Protein on the Performance of Nontarget Leafhopper,Nephotettix cincticeps(Hemiptera: Cicadellidae), Under Laboratory and Field Conditions

Cited by 16 publications

References 51 publications

Cry1Ab-expressing rice did not influence expression of fecundity-related genes in the wolf spider Pardosa pseudoannulata

Cry1Ab-expressing rice did not influence expression of fecundity-related genes in the wolf spider Pardosa pseudoannulata

Combined influence of Bt rice and rice dwarf virus on biological parameters of a non-target herbivore, Nephotettix cincticeps (Uhler) (Hemiptera: Cicadellidae)

The development and status of Bt rice in China

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