Impacts of combining Limosilactobacillus reuteri KUB‐AC5 and Limosilactobacillus fermentum KUB‐D18 on overweight gut microbiota using a simulated human colon model

Abstract

SummaryHigh‐fat diets lead to fat accumulation and contribute to the incidence of obesity, which has now become a global public health problem. High‐fat diets directly impact the microbial composition of the gastrointestinal tract, causing an imbalance in the gut microbiota. This study investigated the effect of probiotics Limosilactobacillus reuteri KUB‐AC5 and Limosilactobacillus fermentum KUB‐D18 in reducing the negative effects associated with the consumption of a high‐fat diet. Results of metagenomics analysis demonstrated that the probiotics regulated the abundance of Enterobacteriaceae, a recognised biomarker of gut dysbiosis. The other observation was the induction of beneficial microbes in the family Bifidobacteriaceae. Supplementation with probiotics in a high‐fat diet significantly reduced phenol, the toxic compound produced by gut microbes (P = 0.032), and promoted the production of beneficial metabolites, including trans‐cinnamic acid and L‐(−)‐3‐phenyllactic acid (P = 0.002 and 0.007, respectively). Results suggested the potential benefits of L. reuteri KUB‐AC5 and L. fermentum KUB‐D18 to alleviate the negative health risks of obesity associated with the consumption of a high‐fat diet. These probiotics offer potential benefits as future health management alternatives.