Trinitrotoluene (TNT), a commonly used explosive for military and industrial applications, can cause serious environmental pollution. 28-day laboratory pot experiment was carried out applying bioaugmentation using laboratory selected bacterial strains as inoculum, biostimulation with molasses and cabbage leaf extract, and phytoremediation using rye and blue fenugreek to study the effect of these treatments on TNT removal and changes in soil microbial community responsible for contaminant degradation. Chemical analyses revealed significant decreases in TNT concentrations, including reduction of some of the TNT to its amino derivates during the 28-day tests. The combination of bioaugmentation-biostimulation approach coupled with rye cultivation had the most profound effect on TNT degradation. Although plants enhanced the total microbial community abundance, blue fenugreek cultivation did not significantly affect the TNT degradation rate. The results from molecular analyses suggested the survival and elevation of the introduced bacterial strains throughout the experiment. Reference to this paper should be made as follows: Nõ lvak, H.; Truu, J.; Limane, B.; Truu, M.; Cepurnieks, G.; Bartkevičs, V.; Juhanson, J.; Muter, O. 2013. Microbial community changes in TNT spiked soil bioremediation trial using biostimulation, phytoremediation and bioaugmentation, Journal of Environmental Engineering and Landscape Management 21(3): 153Á162. http://dx.
abstract.Clay geofiltration studies became important in Lithuania when the clays began to be used as geofiltration barriers for waste disposal sites/repositories. The clay is planned to be used also in construction of the future radioactive waste repository for the Ignalina Nuclear Power Plant. The results of investigation on Stabatiškė subformation till clay occurring at the base of the repository are given in the paper. The investigation has been performed with the purpose to determine potential dependence of clay filtration coefficient values on the number of freeze-thaw cycles and duration of filtration. The filtration coefficient for the repository till clay has been found to be greatly varying at the start of the filtration and during the experiment that lasted several days. This confirms that, during the filtration, structural changeover of clay-forming aggregates takes place with pore space volumes changing and affecting the filtration coefficient values. The filtration parameters are even more affected by freeze-thaw cycles and their number. When the clay thaw is finished the colmatation of fissure system is very slow and incomplete.