Impacts of Matrix Metalloproteinase 9 Genotypes on Renal Cell Carcinoma

Abstract

Background/Aim: The expression of matrix metalloproteinase 9 (MMP9) is elevated in various renal diseases, including renal cell carcinoma. However, the role of MMP9 genotype in this context remains unclear. This study aimed to investigate the association between MMP9 promoter rs3918242 genotypes and the risk of renal cell carcinoma. Materials and Methods: The MMP9 rs3918242 genotypes of 118 patients with renal cell carcinoma and 590 healthy subjects were determined using the polymerase chain reaction-restriction fragment length polymorphism method. Results: The results indicated that individuals carrying the CT or TT genotype of MMP9 rs3918242 did not exhibit an increased risk of renal cell carcinoma compared to wild-type CC carriers (odds ratio=1.20 and 2.68, 95% confidence interval=0.75-1.92 and 0.89-8.03; p=0.5270 and 0.1420, respectively). However, individuals with the CT and TT genotypes had a higher prevalence of renal cell carcinoma than those with the CC genotype when they also had hypertension (p=0.0010), diabetes (p=0.0010), or a family history of cancer (p<0.00001). No correlation was observed between MMP9 rs3918242 genotypic distribution and age (60 years or younger vs. older than 60 years) or sex (both p>0. 05). Additionally, no correlation was found between MMP9 rs3918242 genotype and the risk of renal cell carcinoma in individuals with smoking or alcohol consumption habits. Conclusion: Carrying the T allele for MMP9 rs3918242 may predict a higher risk of renal cell carcinoma among individuals diagnosed with hypertension, diabetes, or with a family history of cancer.Renal cell carcinoma, responsible for approximately 80% of all renal tumors (1), holds the distinction of being worldwide the most predominant malignant solid tumor affecting the kidneys (2, 3). Evidence from epidemiological studies suggests that several factors may contribute to the development of renal cell carcinoma. These factors include diabetes (4, 5), alcoholism (6-8), cigarette consumption (9), hypertension (4, 10) and aging (11-13), as supported by scientific literature. The identification of reliable genomic markers for renal cell carcinoma has been slow and unsatisfactory, despite the existence of several protein markers used to assess the risk of this condition. This delay can be attributed to various factors, including the relatively low prevalence of renal cell carcinoma compared to other 2452

The Contribution of Tissue Inhibitor of Metalloproteinase-2 Genotypes to Breast Cancer Risk in Taiwan

“…Peripheral blood was collected from all participants, and their DNA was extracted within 24 h and stored according to our routine protocol [32,33]. Genotyping for TIMP-2 rs8179090, rs4789936, rs2009196, and rs7342880 was performed using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) methodology as we previously published [34].…”