Impacts of Oxidative Stress and Antioxidants on Semen Functions

Bansal, Ashish; Bilaspuri, G. S.

doi:10.4061/2011/686137

Cited by 506 publications

(476 citation statements)

References 61 publications

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“…Our sperm samples were fresh ejaculates, so the conditions causing the defect must be related to the physiology of the male. Several factors are known to induce premature acrosome reaction in birds and mammals, including mutations [19], extracellular calcium and lead [20,21], and oxidative stress [22]. Interestingly, a link between oxidative stress and poor sperm performance has already been indicated in barn swallows from Chernobyl [11,23], so rsbl.royalsocietypublishing.org Biol Lett 9: 20130530 we speculate that premature acrosome reaction is caused by oxidative stress and accelerated sperm senescence [24].…”

Section: Discussionmentioning

confidence: 99%

Patterns of sperm damage in Chernobyl passerine birds suggest a trade-off between sperm length and integrity

et al. 2013

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Interspecific variation in sperm size is enigmatic, but generally assumed to reflect species-specific trade-offs in selection pressures. Among passerine birds, sperm length varies sevenfold, and sperm competition risk seems to drive the evolution of longer sperm. However, little is known about factors favouring short sperm or constraining the evolution of longer sperm. Here, we report a comparative analysis of sperm head abnormalities among 11 species of passerine bird in Chernobyl, presumably resulting from chronic irradiation following the 1986 accident. Frequencies of sperm abnormalities varied between 15.7 and 77.3% among species, more than fourfold higher than in uncontaminated areas. Nonetheless, species ranked similarly in sperm abnormalities in unpolluted areas as in Chernobyl, pointing to intrinsic factors underlying variation in sperm damage among species. Scanning electron microscopy of abnormal spermatozoa revealed patterns of acrosome damage consistent with premature acrosome reaction. Sperm length, but not sperm competition risk explained variation in sperm damage among species. This suggests that longer spermatozoa are more susceptible to premature acrosome reaction. Therefore, we hypothesize a trade-off between sperm length and sperm integrity affecting sperm evolution in passerine birds.

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Section: Discussionmentioning

confidence: 99%

Patterns of sperm damage in Chernobyl passerine birds suggest a trade-off between sperm length and integrity

et al. 2013

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“…The toxicity of ROS is responsible for several cellular alterations (Soares et al, 2014) also, productions of ROS decrease sperm motility (Ogbuewu et al, 2010). Because of lipid peroxidation, the plasma membrane decrease fluidity and integrity (Bansal and Bilaspuri, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…However, oxidative stress has been associated with male infertility (Bansal and Bilaspuri, 2010;El-Tohamy, 2012). The toxicity of ROS is responsible for several cellular alterations (Soares et al, 2014) also, productions of ROS decrease sperm motility (Ogbuewu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Oxidative Status of Boar Semen during Storage

Zanella¹,

Zanella²,

Poetini³

et al. 2016

American Journal of Biochemistry and Biotechnology

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Abstract:The intensification of the use of artificial insemination in the swine industry has increased the interest to develop proper conditions to store semen better for longer periods of time without affecting its fertility. Trolox is a vitamin E analog with antioxidant property to prevent oxidative stress. Therefore, the objective of this study was to evaluate the Trolox antioxidant capacity on boar semen during storage period (86 h) at 15°C. For that, semen samples from 5 different boars were collected and diluted with a Long-Term (LT) extender with and without the addition of Trolox in a concentration of 50 µM. The sperm motility, oxidative stress and antioxidant protection were measured in those samples during an 84 h storage time. Our results indicated that Trolox was able to minimize the loss of sperm motility after 24 h of storage. The storage time was positively correlated with TBARS production. However in the presence of Trolox, after 12 h, TBARS content were significantly lower than those of sperm cells stored with LT diluent only. The concentrations of Non-Protein SH groups (NPSH), after 36 h of storage, were lower in sperm cells stored only with LT diluent when compared to the group supplemented with Trolox. Catalase and Superoxide Dismutase activities were decreased after 48 h storage at 15°C in both groups (with or without Trolox). Spontaneous spermatozoa ROS formation was high in viable swine sperm during the LT storage period in samples without Trolox. In conclusion, LT diluent with the addition of antioxidant as Trolox can reduce the oxidative stress in sperm cells, which could be used to protect spermatozoa against oxidative damage, preventing motility losses in swine semen.

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“…However, the high performance of GPx activity depends on several factors, including its biodisponibility, levels of reduced glutathione (GSH) and presence of selenium, an important component of GPx active site (Jurkovic et al, 2008). If the GPx system is exhausted and becomes unable to purge the H 2 O 2 produced, the excess of this ROS may react with polyunsaturated fatty acids present in the sperm membrane, inducing lipid peroxidation (Morliere & Santus, 1998;Bansal & Bilaspuri, 2010). Furthermore, the remaining H 2 O 2 in the presence of transition metals, can be converted by the Fenton reaction in hydroxyl radical (Gutteridge, 1986;Denisov & Afanas'ev, 2005), considered an unstable ROS and thus mainly responsible for oxidative damage to biomolecules (i.e., proteins, lipids, DNA) (Aprioku, 2013;Meyerstein, 2013).…”

Section: (A) (B) (C)mentioning

confidence: 99%

Epididymal contraction and sperm parameters are affected by clonidine

et al. 2014

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SUMMARYThe use of clonidine, a selective agonist of a 2 -adrenoceptors, is related to the fertility impairment. Thus, it has been described that changes in the epididymal function are related to the loss of fertility. Therefore, this study was sought to further evaluate the effects of clonidine in the rat distal cauda epididymis contractions and its consequence in the sperm parameters. The in vitro effects of clonidine in the isolated distal cauda epididymis were evaluated by pharmacological experiments. The consecutive contractile responses for clonidine in distal cauda epididymis showed desensitization. The noradrenaline-induced contractions were desensitized after in vitro clonidine pre-treatment (10 À5 M for 10 min). Clonidine was unable to alter the noradrenaline contractions if the in vitro pre-treatment was made in the presence of idazoxan (a 2 -adrenoceptor antagonist), whereas prazosin (a 1 -adrenoceptor antagonist) was ineffective. Moreover, the in vitro clonidine pre-treatment increased frequency and amplitude of spontaneous contraction of distal cauda epididymis. In addition, to induce in vivo desensitization of a 2 -adrenoceptors, male Wistar rats were treated with crescent doses of clonidine and distal cauda of epididymis contraction and sperm parameters were analyzed. The in vivo treatment with clonidine diminished the potency of the contractions induced by adrenergic agonists and augmented the frequency and amplitude of spontaneous contraction of distal cauda epididymis. This treatment also altered the sperm transit time in epididymis, epididymal sperm reserves, sperm lipid peroxidation, and antioxidant enzymes activity. The results suggest that clonidine was able to affect the sperm quantity and quality by decreasing the transit time related to the increase in the frequency and amplitude of spontaneous contractions in epididymis, although the contractions induced by adrenergic agonists were desensitized.

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Impacts of Oxidative Stress and Antioxidants on Semen Functions

Cited by 506 publications

References 61 publications

Patterns of sperm damage in Chernobyl passerine birds suggest a trade-off between sperm length and integrity

Patterns of sperm damage in Chernobyl passerine birds suggest a trade-off between sperm length and integrity

Oxidative Status of Boar Semen during Storage

Epididymal contraction and sperm parameters are affected by clonidine

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