Impacts of parturition and body condition score on glucose uptake capacity of bovine monocyte subsets

Eger, Melanie; Hussen, Jamal; Drong, C.; Meyer, Ulrich; Soosten, Dirk von; Frahm, Jana; Daenicke, S.; Breves, Gerhard; Schuberth, Hans‐Joachim

doi:10.1016/j.vetimm.2015.04.007

Cited by 30 publications

(37 citation statements)

References 38 publications

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“…The identification of camel monocyte subsets and the analysis of the subset-specific expression pattern of monocytic markers and adhesion molecules were performed after direct and indirect labeling of cells with surface molecule-specific antibodies and flow cytometrical analysis [18]. Separated camel leukocytes (5 x 10 6 cells / ml) were incubated in 96 well round-bottom microtiter plates (1 x 10 6 / well; 20 min; 4°C), in a three-step staining process, with monoclonal antibodies specific for CD172a, MHCII and CD163 (in the following two combinations: CD172a; MHCII; CD14 and CD163; MHCII; CD14 ) or with isotype control antibodies in PBS containing bovine serum albumin (5 g / L) and NaN 3 (0.1 g / L).…”

Section: Flow Cytometric Analysis Of Camel Blood Monocytesmentioning

confidence: 99%

Dromedary camel CD14high MHCIIhigh monocytes display inflammatory properties and are reduced in newborn camel calves

Hussen

Shawaf

Al-Mubarak

et al. 2019

Preprint

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Background in human and different animal species, blood monocytes are classified based on their expression pattern of different monocytic markers into phenotypically and functionally different subsets. In the current study, we used monoclonal antibodies to CD172a, CD14, CD163 and MHCII to identify monocyte subsets in peripheral blood of dromedary camels and to evaluate their phenotype and function.Results similar to bovine and swine monocytes, camel CD172a can be used as a pan-monocyte marker. Based on CD14, CD163 and MHCII expression, camel CD172a+ monocytes were divided into three subsets: The major subpopulation of camel monocytes (mo-I) showed high expression of CD14 and CD163, but low expression of MHCII. A second subset of monocytes (mo-II) expressed highly all three markers, CD14, CD163 and MHCII. A third monocyte subset (mo-III) displayed low expression of CD14 and CD163 with high MHCII expression. The three monocyte subsets also showed different expression patterns of cell adhesion molecules. While the two MHCII high subsets (mo-II and mo-III) showed higher expression of CD11a in comparison to the MHCIIlow subset mo-I, CD18 and CD11b were highest expressed on the two CD14high subsets (mo-I and mo-II). Bacterial stimulation of camel leukocytes identified mo-II cells as an antimicrobial monocyte subset with the highest phagocytic and ROS production capacity. The comparison of monocyte counts and phenotype between newborn camel calves and adult camels revealed significantly reduced numbers of mo-II cells in newborn animals. Monocytes of newborns expressed significantly more CD172a and CD163 molecules but less CD14 and MHCII molecules than monocytes of adult camels.Conclusions The analysis of phenotypic and functional properties suggests that camel monocyte subsets mo-I, mo-II and mo-III are counterparts of bovine classical, intermediate and non-classical monocytes respectively. Age related changes in camel monocyte numbers and phenotype were identified.

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Section: Flow Cytometric Analysis Of Camel Blood Monocytesmentioning

confidence: 99%

Dromedary camel CD14high MHCIIhigh monocytes display inflammatory properties and are reduced in newborn camel calves

Hussen

Shawaf

Al-Mubarak

et al. 2019

Preprint

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“… k Based on gene expression analysis in bovine monocytes ( 19 ) . l Based on gene expression analysis in bovine monocytes ( 30 ) . …”

Section: Phenotypic Heterogeneity Of Bovine Monocyte Subsetsmentioning

confidence: 99%

Heterogeneity of Bovine Peripheral Blood Monocytes

Hussen

Schuberth

2017

Front. Immunol.

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Peripheral blood monocytes of several species can be divided into different subpopulations with distinct phenotypic and functional properties. Herein, we aim at reviewing published work regarding the heterogeneity of the recently characterized bovine monocyte subsets. As the heterogeneity of human blood monocytes was widely studied and reviewed, this work focuses on comparing bovine monocyte subsets with their human counterparts regarding their phenotype, adhesion and migration properties, inflammatory and antimicrobial functions, and their ability to interact with neutrophilic granulocytes. In addition, the differentiation of monocyte subsets into functionally polarized macrophages is discussed. Regarding phenotype and distribution in blood, bovine monocyte subsets share similarities with their human counterparts. However, many functional differences exist between monocyte subsets from the two species. In contrast to their pro-inflammatory functions in human, bovine non-classical monocytes show the lowest phagocytosis and reactive oxygen species generation capacity, an absent ability to produce the pro-inflammatory cytokine IL-1β after inflammasome activation, and do not have a role in the early recruitment of neutrophils into inflamed tissues. Classical and intermediate monocytes of both species also differ in their response toward major monocyte-attracting chemokines (CCL2 and CCL5) and neutrophil degranulation products (DGP) in vitro. Such differences between homologous monocyte subsets also extend to the development of monocyte-derived macrophages under the influence of chemokines like CCL5 and neutrophil DGP. Whereas the latter induce the differentiation of M1-polarized macrophages in human, bovine monocyte-derived macrophages develop a mixed M1/M2 macrophage phenotype. Although only a few bovine clinical trials analyzed the correlation between changes in monocyte composition and disease, they suggest that functional differences between human and bovine monocyte subsets are also reflected in their different clinical relevance for distinct diseases. In opposite to the human system, where higher blood cell number of non-classical monocytes was widely correlated with several human infectious and non-infectious diseases, higher counts of bovine intermediate monocytes are suggested as a potential biomarker for inflammatory responses postpartum.

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“…Under different pathological (e.g. infection) (Duvel et al, 2014) and physiological conditions (pregnancy) (Eger et al, 2015), distribution of T cell subpopulations in blood can change significantly. Age-related changes in the composition of peripheral blood T cells were reported for different species (Romanyukha and Yashin, 2003;Elghetany and Lacombe, 2004).…”

Section: Discussionmentioning

confidence: 99%

Expression Patterns of Cell Adhesion Molecules on CD4+ T Cells and WC1+ T Cells in the Peripheral Blood of Dromedary Camels

Hussen¹

2018

PVJ

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In human, mice and different veterinary species, T cells are subdivided into phenotypically different subpopulations based on their expressed T cell receptor and cell specific surface markers. As no data were available regarding the existence of T cell subpopulations in the peripheral blood of dromedary camels, the current study aimed at the identification of camel blood T cell subpopulations and the analysis of their adhesion molecules expression patterns. Using monoclonal antibodies crossreactive with camel CD antigens, camel blood WC1-positive T cells and CD4positive  T cells and their expression pattern of adhesion molecules were identified by flow cytometry. Under camel CD4-positive T cells, a major fraction (83%) with naïve phenotype (CD4 + CD11a lo CD44 lo) and minor fraction (13%) with effector phenotype (CD4 + CD11a hi CD44 hi) were identified. In addition, the analysis of age related effects on the proportion of lymphocyte subsets indicates that camels belong to the T cell high species.

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Impacts of parturition and body condition score on glucose uptake capacity of bovine monocyte subsets

Cited by 30 publications

References 38 publications

Dromedary camel CD14high MHCIIhigh monocytes display inflammatory properties and are reduced in newborn camel calves

Dromedary camel CD14high MHCIIhigh monocytes display inflammatory properties and are reduced in newborn camel calves

Heterogeneity of Bovine Peripheral Blood Monocytes

Expression Patterns of Cell Adhesion Molecules on CD4+ T Cells and WC1+ T Cells in the Peripheral Blood of Dromedary Camels

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