Impacts of pile temperature on antibiotic resistance, metal resistance and microbial community during swine manure composting

Wu, Nan; Xie, Shouqi; Zeng, Ming; Xu, Xiao; Li, Yan; Liu, Xinyuan; Wang, Xiaobo

doi:10.1016/j.scitotenv.2020.140920

Cited by 69 publications

(16 citation statements)

References 37 publications

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“…In support of this finding, early studies have demonstrated that bacteria carrying tetracycline ARGs were less adaptive than those carrying other ARGs in the curing phase when the temperature and antibiotic concentrations reduced, probably because the former primarily consisted of enteric bacteria that appeared to diminish during composting. 12,77 By contrast, the enrichment of multidrug and beta-lactam ARGs might be primarily attributed to a wide range of host bacteria and their proliferation 23,78,79 during composting. Although the current literature pertaining to composting efficiency at ARG elimination is contradictory, 8 our study collectively demonstrates a selective role of composting in influencing ARGs and points to an enrichment of multidrug and beta-lactam ARGs in the curing phase of multiple manure composting processes.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Disentangling the Effects of Physicochemical, Genetic, and Microbial Properties on Phase-Driven Resistome Dynamics during Multiple Manure Composting Processes

Cheng

Zhu

Strong

et al. 2021

Environ. Sci. Technol.

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Composting alters manure-derived antibiotic resistance genes (ARGs) to a certain extent, which is largely dependent upon the composting phase, manure type, microbial phylogeny, and physicochemical properties. However, little is known about how these determinants influence the fate and dynamics of ARGs as well as the mechanisms underlying the ecological process of ARGs during composting. Here, we investigated the temporal patterns of ARGs and their correlations with a series of physicochemical, genetic, and microbial properties during pilotscale composting of chicken, maggot, bovine, and swine manure. We detected 237 ARGs, 71 of which were co-occurring across all four composting processes and accounted for >80% of the sum of resistome abundance. In support of this ARG co-occurrence, variance partition analyses demonstrated that the manure type explained less resistome variations (5.6%) than the composting phase (21.6%). During the phase-driven resistome dynamics, ARGs showed divergent variations in abundance, and certain beta-lactams and multidrug ARGs were consistently enriched across multiple manure composting processes. Correlation analyses all led to the conclusion that the divergent ARG variations during composting were attributable to the unequal effects of physicochemical properties, mobile elements, and succession of indigenous microbiota, whereas antibiotic residues' effects were marginal. Ultimately, this study determines the relative importance of various key determinants in the phase-driven divergence of ARGs during multiple manure composting processes and demonstrates a clear need to evaluate risks posed by enriched ARGs toward their receiving environments.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Disentangling the Effects of Physicochemical, Genetic, and Microbial Properties on Phase-Driven Resistome Dynamics during Multiple Manure Composting Processes

Cheng

Zhu

Strong

et al. 2021

Environ. Sci. Technol.

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“…Employing metagenomics for the analysis of swine manure composting, it has been reported that the host microbiota influences the depletion of these genes more than the environmental factors of the process itself (Zhang et al, 2018). Furthermore, in the disappearance of some genera such as Acinetobacter or Pseudomonas could also be related (Wu et al, 2020). Therefore, the possibility of corroborating these promising results on industrial composting of sewage sludge is of significant relevance.…”

Section: Introductionmentioning

confidence: 99%

Industrial Composting of Sewage Sludge: Study of the Bacteriome, Sanitation, and Antibiotic-Resistant Strains

et al. 2021

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Wastewater treatment generates a huge amount of sewage sludge, which is a source of environmental pollution. Among the alternatives for the management of this waste, industrial composting stands out as one of the most relevant. The objective of this study was to analyze the bacterial population linked to this process and to determine its effectiveness for the reduction, and even elimination, of microorganisms and pathogens present in these organic wastes. For this purpose, the bacteriome and the fecal bacteria contamination of samples from different sewage sludge industrial composting facilities were evaluated. In addition, fecal bacteria indicators and pathogens, such as Salmonella, were isolated from samples collected at key stages of the process and characterized for antibiotic resistance to macrolide, β-lactam, quinolone, and aminoglycoside families. 16S rRNA phylogeny data revealed that the process clearly evolved toward a prevalence of Firmicutes and Actinobacteria phyla, removing the fecal load. Moreover, antibiotic-resistant microorganisms present in the raw materials were reduced, since these were isolated only in the bio-oxidative phase. Therefore, industrial composting of sewage sludge results in a bio-safe final product suitable for use in a variety of applications.

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“…Some studies have reported that continuous high temperature could promote the removal of ARGs during composting ( Wang et al, 2016 ; Xie et al, 2016 ; Youngquist et al, 2016 ), and facilitate antibiotics removal through degradation and adsorption ( Arikan et al, 2007 ). However, some studies have indicated that the effect of high temperature on ARGs removal are not ideal ( Huang et al, 2019 ; Wu et al, 2020 ). It can be seen that whether high temperature can remove ARGs is controversial.…”

Section: Introductionmentioning

confidence: 99%

Effect of biochar on antibiotics and antibiotic resistance genes variations during co-composting of pig manure and corn straw

Tong

Liu

Tian

et al. 2022

Front. Bioeng. Biotechnol.

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Pig manure is a reservoir of antibiotics and antibiotic resistance genes (ARGs). The effect of biochar on the variations in physicochemical properties, bacterial communities, antibiotics, ARGs, and mobile genetic elements (MGEs) of compost product during co-composting of pig manure and corn straw have been investigated in this study. Compared with the control treatment (CK), biochar addition accelerated the increase in pile temperature and prolonged the high temperature period (>55°C) for 2 days. Under biochar influence, organic matter degradation, NH4+-N conversion and NO3−-N production was accelerated, and dissolved total organic carbon (DOC) and dissolved total nitrogen (DTN) utilization by microorganisms were enhanced. Biochar addition altered the microbial community and promoted the vital activity of Actinobacteria in the later composting stage. The antibiotics removal efficiency (except danofloxacin and enrofloxacin) was accelerated in the early composting stage (1–14 days) by biochar addition, the pile temperature had a positive effect on antibiotics removal, and the total antibiotics removal efficiency in CK and CK+Biochar treatments was 69.58% and 78.67% at the end of the composting process, respectively. The absolute abundance of most of the ARGs in the CK+Biochar treatment was lower than that in the CK treatment during composting, and the ARGs removal mainly occurred in the early (1–14 days) and later (28–50 days) stages. Biochar addition reduced the absolute abundance of MGEs (intI1, intI2) in the compost product, and most of the ARGs had a significant positive correlation with MGEs. Network analysis and redundancy analysis showed that ARGs and MGEs occurred in various host bacteria (Firmicutes, Actinobacteria, Bacteroidetes, Proteobacteria, and Halanaerobiaeota), and that DTN and NH4+-N are the main factors regulating the changes in bacterial communities, antibiotics, ARGs, and MGEs during composting. Moreover, MGEs contributed the most to the variation in ARGs. In summary, biochar addition during composting accelerated antibiotics removal and inhibited accumulation and transmission of ARGs. The results of this study could provide theoretical and technical support for biochar application for antibiotics and ARGs removal during livestock and poultry manure composting.

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Impacts of pile temperature on antibiotic resistance, metal resistance and microbial community during swine manure composting

Cited by 69 publications

References 37 publications

Disentangling the Effects of Physicochemical, Genetic, and Microbial Properties on Phase-Driven Resistome Dynamics during Multiple Manure Composting Processes

Disentangling the Effects of Physicochemical, Genetic, and Microbial Properties on Phase-Driven Resistome Dynamics during Multiple Manure Composting Processes

Industrial Composting of Sewage Sludge: Study of the Bacteriome, Sanitation, and Antibiotic-Resistant Strains

Effect of biochar on antibiotics and antibiotic resistance genes variations during co-composting of pig manure and corn straw

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