Impacts of rumen fluid, refrigerated or reconstituted from a refrigerated pellet, on gas production measured at 24h of fermentation

Fabro, Carla; Sarnataro, Chiara; Spanghero, M.

doi:10.1016/j.anifeedsci.2020.114585

Cited by 7 publications

(14 citation statements)

References 17 publications

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“…The influence of storage time and temperature on the ability of rumen microorganisms to degrade NDF has been examined by other authors [15]. They reported that, withinday delays up to 6.5 h between the time of collection of the rumen inoculum and the beginning of the incubation, no effects were observed on the in vitro digestion of NDF when RF was maintained under anaerobic conditions at 39 • C. Some authors [7,15,19,20] have demonstrated that RF, or mixed ruminal microorganisms, can also be stored at low temperature without negative effects on gas production and some fermentation parameters.…”

Section: Resultsmentioning

confidence: 99%

Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials

Fortina

Patrucco

Barbera

et al. 2022

MPs

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Digestibility trials need a viable rumen fluid as inoculum to degrade feeds. The variability of rumen fluid depends on the animal’s diet, while its viability is greatly influenced by the sampling and handling procedures. In this article, we present a replicable protocol for sampling the rumen fluid from slaughtered animals for in vitro digestibility trials. A detailed list of the tools and a step-by-step standardized procedure for the collection, storage and the transportation of the rumen fluid from the slaughterhouse to the laboratory is presented. We also describe a digestibility trial for establishing the maximum storage time of rumen fluid from sampling to its use. The results show that the rumen fluid, collected and maintained according to the proposed protocol, can be stored and used from 30 to 300 min from sampling without significantly compromising the fermentative activity of the microbial population.

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Section: Resultsmentioning

confidence: 99%

Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials

Fortina

Patrucco

Barbera

et al. 2022

MPs

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“…This low accumulation of VFA may be caused by the decreasing ruminal microbes activity. Fabro et al (2020) stated that rumen liquid has an impact on gas production. S. guianensis produced the highest average total gas production which was in line with the total concentration of VFA produced.…”

Section: Discussionmentioning

confidence: 99%

Sugar, Acid Soluble Polysaccharide, and Total Phenolic Contents in Tropical Legumes and Their Relationships with In Vitro Nutrient Fermentability

Ikhwanti

Jayanegara

Permana

et al. 2020

Trop. Anim. Sci. J.

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Tropical legume is a type of C4 plant that has been adaptive to hot environments. Therefore, tropical legumes require energy reserves in the form of sugar and starch. This study aimed to explain the relationship between sugar, starch, and tannin contents of tropical legumes and their in vitro fermentation profiles. Samples of Bauhinia purpurea, Pterocarpus indicus, Tamarindus indica, Calopogonium mucunoides, Macroptilium atropurpureum, and Stylosanthes guianensis were tested by proximate analysis, Van Soest, and in vitro nutrient fermentability. The in vitro fermentability consisted of dry matter degradability (DMD), organic matter degradability (OMD), volatile fatty acid (VFA), methane and ammonia concentrations, and gas production. The gas production kinetics were determined as gas production from soluble nutrient (a), maximum gas production (a+b), and rate of gas production (c). All samples were tested using the orthogonal contrast test to compare in vitro fermentability characteristics between the different types of legumes and their tannin contents. Results revealed that the average content of the legumes dry matter examined was 22% with CP content of 15% -28%. The legumes in this study had average content of 3.74% sugar, 21.86% ASP, and 0.3% total phenolics (dry matter basis). Acid soluble polysaccharides content in legumes had a positive correlation with degradability, ammonia concentration, and total gas production (p<0.05). However, sugar content did not have a correlation with the other in vitro fermentation characteristics. The exploration of sugar and acid soluble polysaccharides content in tropical legumes might be profitable as these nutrients could increase feeding efficiency. Sugar did not have a significant relationship to the characteristics of fermentation, on the contrary, acid soluble polysaccharides had a very close relationship to the characteristics of fermentation. The total phenolics had a close relationship with the production of VFA and ammonia. Tree legumes have better digestibility than shrub legumes.

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“…It is more convenient reckoning the availability considered limited for live cattle extraction than the slaughtered ones that are procurable guaranteed. Freezing RF prevalent as preservation within 72 hours at 4°C; did not affecting gas production statistically, whilst decreasing at 96 hours [1]. Frozen RF crystallizes that made microbe cells dehydrated decreasing the viability [2].…”

Section: Introductionmentioning

confidence: 94%

“…Immediately transferred into the laboratory, the RF are divided into two freezing methods, gradual (from rumen to room to refrigerator to chiller and to freezer temperature every 4 hours) and immediate, with each freezing method differentiated into 4, 11, and 18 days. The preservation executed in -22°C freezer, and when it is due simulation, thawing at 39°C waterbath is correct according to Fabro et al [1].…”

Section: Preparationmentioning

confidence: 99%

“…However, the gradual freezing GP rate shows a different trend, peaking at 4 days (192.5%), then decreasing at 11 (110%) days and 18 days (92.5%). The different trend happened because of the cold shock which affected the metabolism process and made the immediate freezing score lower at 4 days of preservation [1].…”

Section: Fig 1 Visual Interpretations Of Non-cryoprotected Treatmentsmentioning

confidence: 99%

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The Potential of Frozen Rumen Fluid with Dimethyl Sulfoxide And Glucose As Cryoprotectant on In Vitro Gas Production And Digestibility

Muhammad Djafar,

Sekar Ayu Putri Darmawan,

Suryapratama

et al. 2024

BIO Web Conf.

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As the pressure from the animal welfare community increases, there is an urgent need to find the alternative rumen fluid (RF) source as an essential inoculum for in vitro digestibility studies. The objective of this study is to support the previous studies utilizing fresh RF from abattoir with and without cryoprotectants during the freezing process. A 2x3x3-factorial design was used to ascertain the effectiveness of dimethyl sulfoxide (DMSO) and glucose as cryoprotectants to frozen RF varying from 4, 11, and 18 days to measure the in vitro gas production (IVGP) and digestibility (IVD) of a complete feed consisted of elephant grass and commercial concentrate at 1:1 ratio. factor A being the preservation methods, factor B being the cryoprotectants, and factor C being the preservation time. IVGP shows that the use of DMSO and glucose as cryoprotectants (P1A, P1B, P2A, & P2B) only held back quality performance and made the noncryoprotected frozen RF (P0A) is the most applicable method for preservation that lasts until 18 days. IVD however resulted in the most similar frozen RF treatment with gradual freezing that uses DMSO as cryoprotectant (P1B). Thus, making DMSO and glucose influenced nothing significant to the frozen RF. Maintaining the reduction of animal welfare while simultaneously keeping the data variation low is achievable and efficient.

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Impacts of rumen fluid, refrigerated or reconstituted from a refrigerated pellet, on gas production measured at 24h of fermentation

Cited by 7 publications

References 17 publications

Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials

Rumen Fluid from Slaughtered Animals: A Standardized Procedure for Sampling, Storage and Use in Digestibility Trials

Sugar, Acid Soluble Polysaccharide, and Total Phenolic Contents in Tropical Legumes and Their Relationships with In Vitro Nutrient Fermentability

The Potential of Frozen Rumen Fluid with Dimethyl Sulfoxide And Glucose As Cryoprotectant on In Vitro Gas Production And Digestibility

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