Impacts of the Marine Hatchery Built Environment on Mucosal Microbiome Colonization Across Ontogeny in Yellowtail Kingfish, Seriola Lalandi

Minich, Jeremiah J.; Nowak, Barbara; Elizur, Abigail; Knight, Rob; Fielder, D. Stewart; Allen, Eric E.

doi:10.21203/rs.3.rs-75521/v1

Cited by 3 publications

(2 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to the low microbial overlap between the intestine and water, we found a high microbial overlap between the intestine and feed. Microbial overlaps between the fish intestine and formulated feeds have been reported to be high [50,51] and low [52][53][54][55] in the literature. As discussed earlier, the feed microbiota detected by amplicon sequencing may have primarily originated from inactive microbes.…”

Section: Microbial Overlap Was Low Between the Intestine And Water Bu...mentioning

confidence: 99%

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Gajardo

Jaramillo-Torres

et al. 2022

anim microbiome

View full text Add to dashboard Cite

Background Being part of fish's natural diets, insects have become a practical alternative feed ingredient for aquaculture. While nutritional values of insects have been extensively studied in various fish species, their impact on the fish microbiota remains to be fully explored. In an 8-week freshwater feeding trial, Atlantic salmon (Salmo salar) were fed either a commercially relevant reference diet or an insect meal diet wherein black soldier fly (Hermetia illucens) larvae meal comprised 60% of total ingredients. Microbiota of digesta and mucosa origin from the proximal and distal intestine were collected and profiled along with feed and water samples. Results The insect meal diet markedly modulated the salmon intestinal microbiota. Salmon fed the insect meal diet showed similar or lower alpha-diversity indices in the digesta but higher alpha-diversity indices in the mucosa. A group of bacterial genera, dominated by members of the Bacillaceae family, was enriched in salmon fed the insect meal diet, which confirms our previous findings in a seawater feeding trial. We also found that microbiota in the intestine closely resembled that of the feeds but was distinct from the water microbiota. Notably, bacterial genera associated with the diet effects were also present in the feeds. Conclusions We conclude that salmon fed the insect meal diets show consistent changes in the intestinal microbiota. The next challenge is to evaluate the extent to which these alterations are attributable to feed microbiota and dietary nutrients, and what these changes mean for fish physiology and health.

show abstract

Section: Microbial Overlap Was Low Between the Intestine And Water Bu...mentioning

confidence: 99%

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Gajardo

Jaramillo-Torres

et al. 2022

anim microbiome

View full text Add to dashboard Cite

show abstract

“…In line with previous studies in salmon (46)(47)(48) and other fish species (49-51), we found that microbial overlap between the intestine and water was low in the present study of salmon in freshwater. This may be explained by the fact that dur- (52)(53)(54)(55). As discussed earlier, the feed 346 microbiota detected by amplicon sequencing may have primarily originated from inactive microbes.…”

Section: Microbial Overlap Was Low Between the Intestine And Water But High Between The Intestine And Feedsmentioning

confidence: 94%

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Gajardo

Jaramillo-Torres

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Being part of fish's natural diets, insects have become a realistic, sustainable feed ingredient for aquaculture. While nutritional values of insects have been extensively studied in various fish species, their impact on the fish microbiota remains to be fully explored. In an 8-week freshwater feeding trial, Atlantic salmon ( Salmo salar ) were fed either a commercially relevant reference diet or an insect meal diet wherein black soldier fly ( Hermetia illucens ) larvae meal comprised 60% of total ingredients. Microbiota of digesta and mucosa origin from the proximal and distal intestine were collected and profiled along with feed and water samples. Results: The insect meal diet markedly modulated the salmon intestinal microbiota. Overall, the microbial diversity was lower in the digesta of salmon fed the insect meal diet but higher in the mucosa. A group of bacterial genera, dominated by members of the Bacillaceae family, was enriched in salmon fed the insect meal diet, which confirms our previous findings in a seawater feeding trial. We also found that microbiota in the intestine closely resembled that of the feeds but was distinct from the water microbiota. Notably, bacterial genera associated with the diet effects were also present in the feeds. Conclusions: We conclude that salmon fed the insect meal diets show consistent changes in the intestinal microbiota. The next challenge is to evaluate the extent to which these alterations are attributable to feed microbiota and dietary nutrients and what these changes mean for fish physiology and health.

show abstract

Fish microbiomes 101: disentangling the rules governing marine fish mucosal microbiomes across 101 species

Minich

Härer

Vechinski

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Fish are the most diverse and widely distributed vertebrates, yet little is known about the microbial ecology of fishes nor the biological and environmental factors that influence the fish microbiome. The microbiota from 101 species of Southern California marine fishes, spanning 22 orders, 55 families, and 83 genera representing ~25% of local marine fish diversity, was analyzed to identify patterns that explain microbial diversity patterns in a geographical subset of marine fish biodiversity. We compared fish microbiomes (gill, skin, midgut, and hindgut) using alpha, beta, and gamma diversity along with establishing a novel method to estimate microbial biomass (Qiime2 plugin katharoseq). For oceanic fishes from the neritic zone, host size and distance from shore were negatively associated with microbial biomass densities and diversity in the gills. Body site was the strongest driver for beta diversity with strong evidence of phylosymbiosis observed across the gill, skin, and hindgut, but not midgut. The majority of microbes from all fish body sites were of unknown origin but overall sea water generally contributes more microbes to fish mucus compared to marine sediment. In a meta-analysis of vertebrate hindguts (569 species), mammals had the highest gamma diversity when controlling for host species number while fishes had the highest percent of unique microbial taxa (92%). In fishes, the midgut, gill, and skin contains the majority of microbial diversity which collectively can be 5.5 times higher than the hindgut. The composite dataset will be useful to vertebrate microbiome researchers and fish biologists interested in microbial ecology with applications in aquaculture and fisheries management.

show abstract

Impacts of the Marine Hatchery Built Environment on Mucosal Microbiome Colonization Across Ontogeny in Yellowtail Kingfish, Seriola Lalandi

Cited by 3 publications

References 55 publications

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Consistent changes in the intestinal microbiota of Atlantic salmon fed insect meal diets

Fish microbiomes 101: disentangling the rules governing marine fish mucosal microbiomes across 101 species

Contact Info

Product

Resources

About