Evapotranspiration (ET) is a key factor to further our understanding of climate change processes, especially on the Tibetan Plateau, which is sensitive to global change. Herein, the spatial patterns of ET are examined, and the effects of environmental factors on ET at different scales are explored from the years 2000 to 2012. The results indicated that a steady trend in ET was detected over the past decade. Meanwhile, the spatial distribution shows an increase of ET from the northwest to the southeast, and the rate of change in ET is lower in the middle part of the Tibetan Plateau. Besides, the positive effect of radiation on ET existed mainly in the southwest. Based on the environment gradient transects, the ET had positive correlations with temperature ( > 0.85, < 0.0001), precipitation (R > 0.89, p < 0.0001), and NDVI (R > 0.75, p < 0.0001), but a negative correlation between ET and radiation (R = 0.76, p < 0.0001) was observed. We also found that the relationships between environmental factors and ET differed in the different grassland ecosystems, which indicated that vegetation type is one factor that can affect ET. Generally, the results indicate that ET can serve as a valuable ecological indicator.