Impacts of the toxic benthic dinoflagellate Prorocentrum lima on the brown mussel Perna perna: Shell-valve closure response, immunology, and histopathology

Neves, Raquel A. F.; Santiago, Tainá Cristina; Carvalho, Wanderson Fernandes de; Silva, Edson dos Santos; Silva, Patrícia Mirella da; Nascimento, Silvia M.

doi:10.1016/j.marenvres.2019.03.006

Cited by 31 publications

(23 citation statements)

References 105 publications

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“…Similar effects would be caused by a primary inhibition of endocytosis with consequent alterations to lysosomes that would contribute to the impairment of immune and gastrointestinal tissues [41]. The immunomodulatory action of several harmful microalgae or biotoxins was previously highlighted in bivalves, either as immunostimulation (e.g., immune cell recruitment, activation of innate immunity, stimulation of phagocytosis) or as immunosuppression (damage to immune organs, apoptosis after toxin uptake, reduced haemocyte viability, inhibition of phagocytosis), depending on the algal species [45,46,51,52,53,54,55,56,57,58].…”

Section: Discussionmentioning

confidence: 99%

Biological Effects of the Azaspiracid-Producing Dinoflagellate Azadinium dexteroporum in Mytilus galloprovincialis from the Mediterranean Sea

et al. 2019

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Azaspiracids (AZAs) are marine biotoxins including a variety of analogues. Recently, novel AZAs produced by the Mediterranean dinoflagellate Azadinium dexteroporum were discovered (AZA-54, AZA-55, 3-epi-AZA-7, AZA-56, AZA-57 and AZA-58) and their biological effects have not been investigated yet. This study aimed to identify the biological responses (biomarkers) induced in mussels Mytilus galloprovincialis after the bioaccumulation of AZAs from A. dexteroporum. Organisms were fed with A. dexteroporum for 21 days and subsequently subjected to a recovery period (normal diet) of 21 days. Exposed organisms accumulated AZA-54, 3-epi-AZA-7 and AZA-55, predominantly in the digestive gland. Mussels’ haemocytes showed inhibition of phagocytosis activity, modulation of the composition of haemocytic subpopulation and damage to lysosomal membranes; the digestive tissue displayed thinned tubule walls, consumption of storage lipids and accumulation of lipofuscin. Slight genotoxic damage was also observed. No clear occurrence of oxidative stress and alteration of nervous activity was detected in AZA-accumulating mussels. Most of the altered parameters returned to control levels after the recovery phase. The toxic effects detected in M. galloprovincialis demonstrate a clear biological impact of the AZAs produced by A. dexteroporum, and could be used as early indicators of contamination associated with the ingestion of seafood.

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Section: Discussionmentioning

confidence: 99%

Biological Effects of the Azaspiracid-Producing Dinoflagellate Azadinium dexteroporum in Mytilus galloprovincialis from the Mediterranean Sea

et al. 2019

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“…Effects of a simulated bloom of P. lima upon hemocyte functions were tested recently in the mussel Perna perna, and impaired phagocytosis, induced ROS production, and decreased THC were observed (Neves et al, 2019).…”

Section: Effects Of Habs On Bivalve Hemocyte Variables Involved In Immentioning

confidence: 99%

“…The most common tissues affected are epithelia from mantle, gills, digestive gland or foot, and the most common alterations found are: vacuolation, oedema, melanization of gills and mantle (putatively associated with oxidative stress), signs of irritation (mucus production), and digestive tubules sloughing and atrophy (Haberkorn et al, 2010b;Hégaret et al, 2012Hégaret et al, , 2010Hégaret et al, , 2009Lassudrie et al, 2014;Medhioub et al, 2012;Neves et al, 2019). Depending upon the organ, effects upon epithelia can be attributed to: (a) the extracellular compounds released by HABs, and (b) the intracellular toxins released after cell lysis at the end of the bloom or after direct contact with gills, or as a consequence of digestion.…”

Section: Tissue Degeneration and Inflammatory Response Caused By Habsmentioning

confidence: 99%

Effects of marine harmful algal blooms on bivalve cellular immunity and infectious diseases: A review

Lassudrie

Hégaret

Wikfors

et al. 2020

Developmental & Comparative Immunology

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Bivalves were long thought to be "symptomless carriers" of marine microalgal toxins to human seafood consumers. In the past three decades, science has come to recognize that harmful algae and their toxins can be harmful to grazers, including bivalves. Indeed, studies have shown conclusively that some microalgal toxins function as active grazing deterrents. When responding to marine Harmful Algal Bloom (HAB) events, bivalves can reject toxic cells to minimize toxin and bioactive extracellular compound (BEC) exposure, or ingest and digest cells, incorporating nutritional components and toxins. Several studies have reported modulation of bivalve hemocyte variables in response to HAB exposure. Hemocytes are specialized cells involved in many functions in bivalves, particularly in immunological defense mechanisms. Hemocytes protect tissues by engulfing or encapsulating living pathogens and repair tissue damage caused by injury, poisoning, and infections through inflammatory processes. The effects of HAB exposure observed on bivalve cellular immune variables have raised the question of possible effects on susceptibility to infectious disease. As science has described a previously unrecognized diversity in microalgal bioactive substances, and also found a growing list of infectious diseases in bivalves, episodic reports of interactions between harmful algae and disease in bivalves have been published. Only recently, studies directed to understand the physiological and metabolic bases of these interactions have been undertaken. This review compiles evidence from studies of harmful algal Highlights  Review of the main studies on effects of marine harmful algae on bivalve immune cells (hemocytes) and on infectious disease  Harmful algae can modulate host-pathogen interactions in bivalves on various way (increase or decrease the infection)  Some experimental studies report the involvement of cellular immunity and global physiological weakness in the modulations  Other mechanisms possibly involved: HAB effects on bivalve filtration; direct

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“…Only a few studies on the direct effects of DST and PTX on bivalve feeding exist [16][17][18]. The majority of studies on bivalves and DST and PTX are focused on depuration processes [6,17,[19][20][21][22][23][24] or physiological effects [25,26]. The physiological effects on bivalves of other harmful algal toxins have received far more attention [27][28][29][30][31][32][33][34][35][36].…”

mentioning

confidence: 99%

Effects of the DSP-toxic dinoflagellate Dinophysis acuta on clearance and respiration rate of the blue mussel, Mytilus edulis

et al. 2020

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Diarrheic Shellfish Poisoning toxins (DST) are a severe health risk to shellfish consumers and can be a major problem for the shellfish industry. Bivalve molluscs can accumulate DST via ingestion of toxic dinoflagellates like Dinophysis spp., which are the most prominent producers of DST. The effects of DST-containing dinoflagellate Dinophysis acuta on bivalve clearance and respiration rate were investigated in the blue mussel (Mytilus edulis) exposed to different algal densities in a controlled laboratory study. Results showed that M. edulis exposed to D. acuta displayed a reduced clearance rate compared to M. edulis exposed to equivalent bio-volumes of the non-toxic cryptophyte Rhodomonas salina. Furthermore, M. edulis ceased to feed on D. acuta after 1 to 4 h, depending on D. acuta densities. The quickest response was observed at the highest densities of D. acuta. The estimated total amount of DST accumulated in the M. edulis exceeded the regulatory limit for human consumption and furthermore, intoxication of the M. edulis seemed to occur faster at high cell toxicity rather than at high cell density. However, respiration rates were, similar, irrespective of whether M. edulis were fed single diets of R. salina, D. acuta or a mixed diet of both algal species. In conclusion, the DST-containing D. acuta had a severe negative effect on the clearance of M. edulis, which can affect the conditions of the M. edulis negatively. Hence, DST may cause low quality M. edulis, due to reduced feeding when exposed to DST-containing D. acuta. OPEN ACCESSCitation: Nielsen P, Krock B, Hansen PJ, Vismann B (2020) Effects of the DSP-toxic dinoflagellate Dinophysis acuta on clearance and respiration rate of the blue mussel, Mytilus edulis. PLoS ONE 15 (3): e0230176. https://doi.org/10.and project no. 10-078561 (https://ufm.dk/en/researchand-innovation/councils-and-commissions/formercouncils-and-commissions/the-danish-council-forstrategic-research?set_language=en&cl=en) to PN, PJH and BV. The funder had no role in study result in long-time closure of shellfish production areas, which can lead to severe economic consequences for the shellfish industry [2].Known DST-producers include several planktonic dinoflagellate species of the genus Dinophysis and a few epibenthic Prorocentrum species [1,3]. Pelagic Dinophysis spp. have been associated with DSP events in most cases. Okadaic acid (OA) together with its variants dinophysistoxins (DTX) and pectenotoxins (PTX) have all been identified in species of Dinophysis, and to accumulate in filter-feeding bivalves e.g. reviews by [4][5][6]. Mussels accumulate DST and PTX primarily in the digestive gland [7] and a number of studies have shown that DST undergo molecular transformations when ingested by bivalves. OA and DTX are esterified to a range of different fatty acid ester derivatives [6,[8][9][10]. PTX are also esterified but can be further transformed to PTX seco acids and seco acid esters [6,10,11]. The molecular transformation into fatty acid esters and seco acids has been suggested to be...

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Impacts of the toxic benthic dinoflagellate Prorocentrum lima on the brown mussel Perna perna: Shell-valve closure response, immunology, and histopathology

Cited by 31 publications

References 105 publications

Biological Effects of the Azaspiracid-Producing Dinoflagellate Azadinium dexteroporum in Mytilus galloprovincialis from the Mediterranean Sea

Biological Effects of the Azaspiracid-Producing Dinoflagellate Azadinium dexteroporum in Mytilus galloprovincialis from the Mediterranean Sea

Effects of marine harmful algal blooms on bivalve cellular immunity and infectious diseases: A review

Effects of the DSP-toxic dinoflagellate Dinophysis acuta on clearance and respiration rate of the blue mussel, Mytilus edulis

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