Impaired angiogenic differentiation of dental pulp stem cells during exposure to the resinous monomer triethylene glycol dimethacrylate

Schertl, Peter; Volk, Joachim; Perduns, Renke; Adam, Knut; Leyhausen, Gabriele; Bakopoulou, Athina; Geurtsen, Werner

doi:10.1016/j.dental.2018.11.006

Cited by 15 publications

(10 citation statements)

References 56 publications

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“…Meanwhile, the natural-fibrin-like structure of CGF could also enable stem cells to grow better. VEGF plays an important role in angiogenesis by promoting endothelial cell proliferation, increasing vascular permeability, and altering the biological effects of extracellular matrix [50]. Nestin is associated with the pluripotency of neural stem cells and is expressed in both precursors of neurons and glial cells [51].…”

Section: Discussionmentioning

confidence: 99%

The potential application of concentrated growth factor in pulp regeneration: an in vitro and in vivo study

Zhao

et al. 2019

Stem Cell Res Ther

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Background: Concentrated growth factor (CGF), as a natural biomaterial, is known to contain platelets, cytokines, and growth factors to facilitate the healing process, but there has been little information acquired in regenerative endodontics. The purpose of this study was to investigate the effects of CGF on proliferation, migration, and differentiation in human dental stem pulp cells (hDPSCs) exposed to lipopolysaccharide (LPS) in vitro and its potential role in pulp regeneration of the immature teeth in vivo. Methods: In vitro experiments: CGF-conditioned medium were extracted by freeze-dried method. hDPSCs were isolated and identified. The proliferative potential of hDPSCs with different concentration of CGF and LPS was evaluated by Cell Counting Kit-8. Migration capacity was analyzed by Transwell assays, odonto/osteoblastic differentiation was determined by measuring alkaline phosphatase (ALP) activity using ALP staining, and the extent of mineralization was evaluated by using Alizarin red S staining. The mRNA expression level of DMP-1, DSPP, OPN, Runx2, and OCN were determined by quantitative polymerase chain reaction (qPCR). In vivo experiments: CGF were used as root canal filling agent of the immature single-rooted teeth in the beagle dogs. The teeth were then radiographed, extracted, fixed, demineralized, and subjected to histologic analyses at 8 weeks. The newly formed dentine-pulp complex and the development of apical foramen were evaluated by the hematoxylin-eosin (HE) and Masson trichrome technique. Soft tissues were analyzed by immunohistochemical staining of vascular endothelial growth factor (VEGF) and Nestin. Results: In vitro experiments: The cultured cells exhibited the characteristics of mesenchymal stem cell. The treatment of LPS significantly increased the expression of TNF-α, IL-1β, IL-6, and IL-8 in hDPSCs, and CGF inhibited the mRNA expression of IL-8 in LPS-stimulated hDPSCs. The proliferation values of the CGF group in LPS-stimulated hDPSCs were significantly higher than that of the control group from day 3 to day 7 (P < 0.05). In addition, the number of migratory cells of the CGF group was greater than that of the control group at 24 h with or without LPS treatment. ALP activities increased gradually in both groups from day 4 to day 7. The mineralized nodules and the expression of odontogenesis-related genes DMP-1 and DSPP, osteogenesis-related genes OPN, Runx2, and OCN were dramatically enhanced by CGF in LPS-stimulated hDPSCs at days 21 and 28. In vivo experiments: In CGF treated group, the results of radiograph, HE, and Masson trichrome staining showed a continuing developed tooth of the immature teeth in the beagle dogs (i.e., the ingrowth of soft tissues into the root

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Section: Discussionmentioning

confidence: 99%

The potential application of concentrated growth factor in pulp regeneration: an in vitro and in vivo study

Zhao

et al. 2019

Stem Cell Res Ther

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“…Substances that reach the pulp trans-dentally can come into contact with the cells there. It is known that some elutable substances such as TEGDMA may have toxic effects on the dental pulp cells or its dental pulp stem cells [21,22]. For this reason, determination of substances diffusing through dentin tubuli and the elution kinetics from these substances are of toxicological interest.…”

Section: The Potential Adverse Consequences Of Monomer Elutionmentioning

confidence: 99%

An alternate methodology for studying diffusion and elution kinetics of dimethacrylate monomers through dentinal tubules

et al. 2020

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Objectives. Ethoxylated bisphenol A dimethacrylate (bisEMA) is a base monomer in several dental resin composites. It was the main aim of the present study to determine if bisEMA can reach the dental pulp by generally passive diffusion through the coronal dentinal tubules stimulated via eluent liquids surrounding the root structures only. Methods. In 20 human third molar teeth, standard Class-I occlusal cavities were prepared and provided either with an adhesive system alone or additionally with a composite restoration, according to the instructions of the manufacturer. The teeth were placed in an elution chamber such that the elution media only came into contact with the tooth root/tooth base where they were incubated at 37 °C for up to 7 d. Samples were taken after 1, 2, 4 and 7 d. Gas chromatography/mass spectrometry was used to identify bisEMA and other monomers in ethanol/water (3:1) and aqueous eluates. Results. bisEMA was only found in ethanol/water eluates, where the teeth had received a composite restoration. Traces of bisEMA with up to three ethylene oxide units could be detected in these eluates. Depending on the dentin thickness, different elution kinetics of bisEMA were determined. Regardless of the treatment of teeth, triethylene glycol dimethacrylate (TEGDMA) and tetraethylene glycol dimethacrylate (TEEGDMA) were found in ethanolic/aqueous eluates in equal amounts. Most TEGDMA and TEEGDMA diffused through the dentin within the first 24 h. Significance. Depending on the dentin layer thickness, bisEMA was released for varied time periods, resulting in varied concentrations and exposure times for the different cells of the dental pulp. The concentrations of TEGDMA and TEEGDMA were greatest for cells of the dental pulp within the first 24 h.

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“…In our study, we chose DSPP as a marker of odontogenic differentiation. VEGF plays an important role in angiogenesis by promoting endothelial cell proliferation, increasing vascular permeability, and changing the biological effects of the extracellular matrix [19] . In this study, PGP9.5 protein was selected to re ect the nervous system, because it is a speci c marker of neurons and nerve bers, which re ects the degree of damage and repair of neurons and nerve bers [20] .…”

Section: Discussionmentioning

confidence: 99%

The combined application of rat bone marrow mesenchymal stem cells and bioceramic materials in the regeneration of dental pulp-like tissues

Wen

Wang

et al. 2020

Preprint

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Background: To observe the effects of combined application of rat bone marrow mesenchymal stem cells (rBMSCs) and a bioceramic material on pulp-like tissue formation. Methods: Rat incisor root fragments without pulp tissues were prepared and filled with a collagen scaffold seeded with rBMSCs, while one side of the root segment was covered by a bioceramic material (iRoot BP). After culture for 12 h, the root fragments were implanted subcutaneously for 3 months. Hematoxylin and eosin (HE) staining was applied to observe biocompatibility and the formation of pulp-like tissues. Incisor root fragments were divided into three parts (BP1/3 , M1/3, and D1/3) to analyze the area and number of new vessels. Immunohistochemical staining of neuroendocrine marker PGP9.5, dentin sialophosphoprotein ( DSPP ), and vascular endothelial growth factor (VEGF) was applied to observe the formation of pulp-like tissues. Root fragments filled with the collagen scaffold only were used as a control. Results: Three months after implantation, root fragments were collected, which were surrounded by a transparent tissue membrane with a good blood supply. The root fragment cavity was filled with pink vascularized pulp-like tissue. According to HE results, iRoot BP had good biocompatibility with new pulp-like tissues and few infiltrating inflammatory cells. Increases in the number and area of new blood vessels were observed in BP1/3 compared with the other two parts. Expression of PGP9.5 and DSPP showed that the newly formed tissues were similar to normal pulp tissues. Conclusion: iRoot BP has good biocompatibility and increases the number and area of new blood vessels. The combined application of stem cells and bioceramic materials may be a better method for pulp revascularization .

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Impaired angiogenic differentiation of dental pulp stem cells during exposure to the resinous monomer triethylene glycol dimethacrylate

Cited by 15 publications

References 56 publications

The potential application of concentrated growth factor in pulp regeneration: an in vitro and in vivo study

The potential application of concentrated growth factor in pulp regeneration: an in vitro and in vivo study

An alternate methodology for studying diffusion and elution kinetics of dimethacrylate monomers through dentinal tubules

The combined application of rat bone marrow mesenchymal stem cells and bioceramic materials in the regeneration of dental pulp-like tissues

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