Impaired light detection of the circadian clock in a zebrafish melanoma model

Hamilton, Noémie; Diaz-de-Cerio, Natalia; Whitmore, David

doi:10.1080/15384101.2015.1014146

Cited by 14 publications

(10 citation statements)

References 54 publications

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“…While the animal-type CRY2 (zCRY2) and zCRY4 have only weak or no transcriptional repression ability for the CLOCK:BMAL complex, the four animal-type CRY1s have strong transcriptional repression abilities 14 , so may not only be involved in the circadian clock but also in clock-associated functions, such as the photoperiodic response. Although daily zCry expression patterns have been investigated both in vivo [2][3][4][5][6]8,11 and in vitro 7,9,10 , we comparatively reinvestigated the spatiotemporal expression profiles and light-responsiveness of zCrys to test the above hypothesis. We found that zCry1ab expression in the eye changes depending on the day length.…”

Section: Continuedmentioning

confidence: 99%

A photoperiodic time measurement served by the biphasic expression of Cryptochrome1ab in the zebrafish eye

Okano

Saratani

Tamasawa

et al. 2020

Sci Rep

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The zebrafish (Danio rerio) is a model species that is used to study the circadian clock. it possesses light-entrainable circadian clocks in both central and peripheral tissues, and its core circadian factor cryptochromes (CRYs) have diverged significantly during evolution. In order to elucidate the functional diversity and involvement of cRYs in photoperiodic mechanisms, we investigated the daily expression profiles of six Cry transcripts in central (brain and eye) and peripheral (fin, skin and muscle) tissues. the zCry genes exhibited gene-specific diurnal conserved variations, and were divided into morning and evening groups. notably, zCry1ab exhibited biphasic expression profiles in the eye, with peaks in the morning and evening. comparing ocular zCry1ab expression in different photoperiods (18L:6D, 14L:10D, 10L:14D and 6L:18D) revealed that zCry1ab expression duration changed depending on the photoperiod: it increased at midnight and peaked before lights off. zCry1ab expression in constant light or dark after entrainment under long-or short-day conditions suggested that the evening clock and photic input pathway are involved in photoperiod-dependent zCry1ab expression. Laser microdissection followed by qRt-pcR analysis showed that the evening peak of zCry1ab was likely ascribed to visual photoreceptors. These results suggest the presence of an eye-specific photoperiodic time measurement served by zCry1ab.The circadian clock consists of three parts: the input pathway(s), the oscillation system and the output pathway(s). Light is the most important signal that activates the input pathway to synchronize the oscillator with daily external cycles. The oscillator generates time signals to control physiological circadian functions through the output pathway. Internal time signals are integrated with the environmental light signal to measure day length, which triggers seasonal photoperiodic responses in many organisms living in temperate zones 1 .The zebrafish (Danio rerio) circadian clock is one of the most studied among fish species, and is reported to exist in not only the central tissues but also the embryo, larva, peripheral tissues and cultured cells (refs. 2-11 in Table 1 , refs. 12-15 ). These zebrafish circadian clocks are directly light-entrainable and this feature is also seen in the other fish cells 16 in contrast to the mammalian circadian clocks that are not light-entrainable except for those in the eye.Among vertebrate core clock components such as period (PER), CLOCK, BMAL and cryptochrome (CRY) proteins, CRYs have both a wide evolutionary background and divergent molecular functions 6 . CRYs are structurally classified into several groups, and together form a large protein family with the photo repair enzymes, photolyases (PHRs). Among CRY/PHR family proteins, animal-type CRYs (CRY1 and CRY2) play a central role in the circadian clock oscillator. Animal-type CRY1 and CRY2 function as transcriptional repressors in the core loop of the circadian clock oscillation system 17,18 , while fruit fly CRY (...

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Section: Continuedmentioning

confidence: 99%

A photoperiodic time measurement served by the biphasic expression of Cryptochrome1ab in the zebrafish eye

Okano

Saratani

Tamasawa

et al. 2020

Sci Rep

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“…Skin is a complex organ consisted of different cell types. [34] Skin is a complex organ consisted of different cell types. It has to be clarified whether expression levels for clock genes are different among the variety of cell types of the skin.…”

Section: Introductionmentioning

confidence: 99%

Daytime changes of skin biophysical characteristics: A study of hydration, transepidermal water loss, ph, sebum, elasticity, erythema, and color index on middle eastern skin

et al. 2016

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Background:The exposure of skin to ultraviolet radiation and temperature differs significantly during the day. It is reasonable that biophysical parameters of human skin have periodic daily fluctuation. The objective of this study was to study the fluctuations of various biophysical characteristics of Middle Eastern skin in standardized experimental conditions.Materials and Methods:Seven biophysical parameters of skin including stratum corneum hydration, transepidermal water loss, pH, sebum, elasticity, skin color, and erythema index were measured at three time points (8 a.m., 12 p.m. and 4 p.m.) on the forearm of 12 healthy participants (mean age of 28.4 years) without any ongoing skin disease using the CK MPA 580 device in standard temperature and humidity conditions.Results:A significant difference was observed between means of skin color index at 8 a.m. (175.42 ± 13.92) and 4 p.m. (164.44 ± 13.72, P = 0.025), between the pH at 8 a.m. (5.72 ± 0.48) and 4 p.m. (5.33 ± 0.55, P = 0.001) and pH at 12 p.m. (5.60 ± 0.48) and 4 p.m. (5.33 ± 0.55, P = 0.001). Other comparisons between the means of these parameters at different time points resulted in nonsignificant P values.Conclusion:There are daytime changes in skin color index and pH. Skin color index might be higher and cutaneous pH more basic in the early morning compared to later of the day.

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“…There is also an abundance of epidemiological data showing a link between shift work and cancer incidence in humans [76]. Zebrafish have also added to this area of investigation [77]. Using luminescent report animals, containing the per3-luciferase gene, it is possible to monitor clock function in a developing melanoma, versus that in neighbouring healthy tissue.…”

Section: The Outputsmentioning

confidence: 99%

Circadian Clocks in Fish—What Have We Learned so far?

Steindal

Whitmore

2019

Biology

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Zebrafish represent the one alternative vertebrate, genetic model system to mice that can be easily manipulated in a laboratory setting. With the teleost Medaka (Oryzias latipes), which now has a significant following, and over 30,000 other fish species worldwide, there is great potential to study the biology of environmental adaptation using teleosts. Zebrafish are primarily used for research on developmental biology, for obvious reasons. However, fish in general have also contributed to our understanding of circadian clock biology in the broadest sense. In this review, we will discuss selected areas where this contribution seems most unique. This will include a discussion of the issue of central versus peripheral clocks, in which zebrafish played an early role; the global nature of light sensitivity; and the critical role played by light in regulating cell biology. In addition, we also discuss the importance of the clock in controlling the timing of fundamental aspects of cell biology, such as the temporal control of the cell cycle. Many of these findings are applicable to the majority of vertebrate species. However, some reflect the unique manner in which “fish” can solve biological problems, in an evolutionary context. Genome duplication events simply mean that many fish species have more gene copies to “throw at a problem”, and evolution seems to have taken advantage of this “gene abundance”. How this relates to their poor cousins, the mammals, remains to be seen.

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Impaired light detection of the circadian clock in a zebrafish melanoma model

Cited by 14 publications

References 54 publications

A photoperiodic time measurement served by the biphasic expression of Cryptochrome1ab in the zebrafish eye

A photoperiodic time measurement served by the biphasic expression of Cryptochrome1ab in the zebrafish eye

Daytime changes of skin biophysical characteristics: A study of hydration, transepidermal water loss, ph, sebum, elasticity, erythema, and color index on middle eastern skin

Circadian Clocks in Fish—What Have We Learned so far?

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