Impaired Osteoclastic Function and Linear Bone Erosion Rate in Secondary Hyperparathyroidism Associated with Chronic Renal Failure

Jaworski, Z; Lok, E.; Wellington, J L

doi:10.1097/00003086-197503000-00036

Cited by 32 publications

(9 citation statements)

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“…Our present time-lapse study allowed us to quantify even more characteristics indicating higher aggressiveness: OCs in trench mode can resorb for days (at least 69 h) while OCs in pit mode stop after a median of 13 h; trenches constantly get longer with time and reached a median length of 76 µm, while pits only reached a median diameter of 19 µm during our recording; the linear rate of erosion was on average 2.2 µm/h in trench mode but only 0.8 µm/h in pit mode. Note that these values are of the same order of magnitude as the 1.8 µm/h reported for the elongation rate of Haversian canals in canine ribs ( Jaworski et al, 1975 ), but well below the rates of pure migration (median of 97 µm/h) reported for rabbit OCs on bone slices ( Kanehisa and Heersche, 1988 ). We also show that the higher erosion rate in trench mode is a specific property of this resorption mode and not only related to a possible subtype of OC, since the same OC roughly doubles its erosion speed when switching from pit mode into trench mode ( Fig.…”

Section: Discussionmentioning

confidence: 45%

Time-lapse reveals that osteoclasts can move across the bone surface while resorbing

Søe

Delaissé

2017

Journal of Cell Science

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Bone erosion both demands that the osteoclast resorbs bone matrix and moves over the bone surface. It is widely accepted that these two activities alternate, because they are considered mutually exclusive since resorption is believed to involve an immobilizing seal to the bone surface. However, clear real-time observations are still lacking. Herein, we used specific markers and time-lapse to monitor live the spatiotemporal generation of resorption events by osteoclasts cultured on bone slices. In accordance with the current view, we found alternating episodes of resorption and migration resulting in the formation of clusters of round pits. However, very importantly, we also demonstrate that more than half of the osteoclasts moved laterally, displacing their extracellular bone-resorbing compartment over the bone surface without disassembling and reconstructing it, thereby generating long trenches. Compared to pit events, trench events show properties enabling higher aggressiveness: long duration (days), high erosion speed (two times faster) and long-distance erosion (several 100 µm). Simultaneous resorption and migration reflect a unique situation where epithelial/secretory and mesenchymal/migratory characteristics are integrated into just one cell phenotype, and deserves attention in future research.

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Section: Discussionmentioning

confidence: 45%

Time-lapse reveals that osteoclasts can move across the bone surface while resorbing

Søe

Delaissé

2017

Journal of Cell Science

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“…Bears resume many physiological processes at the onset of remobilization (e.g., waste excretion); bone turnover is probably increased soon after physical activity is resumed following hibernation (McGee et al, 2008). The radial rate of bone resorption by osteoclasts in cortical bone is approximately 9 μm/day (Jaworski et al, 1975), and active bears demonstrate an activation frequency of intracortical remodeling of approximately 0.5 sites/mm 2 /week (McGee et al, 2008); a bear with a 300 mm 2 femoral cross-sectional area could activate approximately 140 new remodeling sites each week, and each site could become a 100–200 μm diameter remodeling cavity in 1–2 weeks. Therefore, even if porosity decreased in the black bears during hibernation (as occurs in hibernating grizzly bears), it is possible that the spring bears experienced some degree of bone resorption during their 1–4 weeks of remobilization which began to elevate porosity.…”

Section: Discussionmentioning

confidence: 99%

Six months of disuse during hibernation does not increase intracortical porosity or decrease cortical bone geometry, strength, or mineralization in black bear (Ursus americanus) femurs

McGee‐Lawrence

Wojda

Barlow

et al. 2009

Journal of Biomechanics

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Disuse typically uncouples bone formation from resorption, leading to bone loss which compromises bone mechanical properties and increases the risk of bone fracture. Previous studies suggest that bears can prevent bone loss during long periods of disuse (hibernation), but small sample sizes have limited the conclusions that can be drawn regarding the effects of hibernation on bone structure and strength in bears. Here we quantified the effects of hibernation on structural, mineral, and mechanical properties of black bear (Ursus americanus) cortical bone by studying femurs from large groups of male and female bears (with wide age ranges) killed during pre-hibernation (fall) and posthibernation (spring) periods. Bone properties that are affected by body mass (e.g. bone geometrical properties) tended to be larger in male compared to female bears. There were no differences (p > 0.226) in bone structure, mineral content, or mechanical properties between fall and spring bears. Bone geometrical properties differed by less than 5% and bone mechanical properties differed by less than 10% between fall and spring bears. Porosity (fall: 5.5 ± 2.2%, spring: 4.8 ± 1.6%) and ash fraction (fall: 0.694 ± 0.011, spring: 0.696 ± 0.010) also showed no change (p > 0.304) between seasons. Statistical power was high (>72%) for these analyses. Furthermore, bone geometrical properties and ash fraction (a measure of mineral content) increased with age and porosity decreased with age. These results support the idea that bears possess a biological mechanism to prevent disuse and age-related osteoporoses.

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“…The mean depth of erosion is about 60 m in trabecular bone and about 100 m in cortical bone. 160 This phase takes about 1 to 3 weeks.…”

Section: Resorptionmentioning

confidence: 99%

Integrated Bone Tissue Physiology

Jee¹

2001

Bone Mechanics Handbook, Second Edition

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Impaired Osteoclastic Function and Linear Bone Erosion Rate in Secondary Hyperparathyroidism Associated with Chronic Renal Failure

Cited by 32 publications

References 0 publications

Time-lapse reveals that osteoclasts can move across the bone surface while resorbing

Time-lapse reveals that osteoclasts can move across the bone surface while resorbing

Six months of disuse during hibernation does not increase intracortical porosity or decrease cortical bone geometry, strength, or mineralization in black bear (Ursus americanus) femurs

Integrated Bone Tissue Physiology

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