Impaired phloem loading in <i>zmsweet13a,b,c</i> sucrose transporter triple knock‐out mutants in <i>Zea mays</i>

Bezrutczyk, Margaret; Hartwig, Thomas; Horschman, Marc; Char, Si Nian; Yang, Jinliang; Yang, Bing; Frommer, Wolf B.; Sosso, Davide

doi:10.1111/nph.15021

Cited by 146 publications

(147 citation statements)

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“…Sucrose allocation and transport in plants have been intensively studied for many years (Li et al ., , ). The seed yields of maize (Bezrutczyk et al ., ) and rapeseed (Luo et al ., ) depend on the efficient allocation of sucrose from the leaves to the seeds. Sucrose also functions as a signal in the regulation of strawberry fruit development and ripening (Jia et al ., ).…”

Section: Resultsmentioning

confidence: 99%

The pomegranate (Punica granatum L.) draft genome dissects genetic divergence between soft‐ and hard‐seeded cultivars

Luo

et al. 2019

Plant Biotechnology Journal

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Summary Complete and highly accurate reference genomes and gene annotations are indispensable for basic biological research and trait improvement of woody tree species. In this study, we integrated single‐molecule sequencing and high‐throughput chromosome conformation capture techniques to produce a high‐quality and long‐range contiguity chromosome‐scale genome assembly of the soft‐seeded pomegranate cultivar ‘Tunisia’. The genome covers 320.31 Mb (scaffold N50 = 39.96 Mb; contig N50 = 4.49 Mb) and includes 33 594 protein‐coding genes. We also resequenced 26 pomegranate varieties that varied regarding seed hardness. Comparative genomic analyses revealed many genetic differences between soft‐ and hard‐seeded pomegranate varieties. A set of selective loci containing SUC8‐like, SUC6, FoxO and MAPK were identified by the selective sweep analysis between hard‐ and soft‐seeded populations. An exceptionally large selective region (26.2 Mb) was identified on chromosome 1. Our assembled pomegranate genome is more complete than other currently available genome assemblies. Our results indicate that genomic variations and selective genes may have contributed to the genetic divergence between soft‐ and hard‐seeded pomegranate varieties.

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Section: Resultsmentioning

confidence: 99%

The pomegranate (Punica granatum L.) draft genome dissects genetic divergence between soft‐ and hard‐seeded cultivars

Luo

et al. 2019

Plant Biotechnology Journal

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“…Two of the key processes for long-distance translocation are phloem loading and seed filling. In maize, SUT1 and SWEET13a,b,c are essential for efficient phloem loading (Slewinski et al, 2009;Bezrutczyk et al, 2018). The transport processes that ultimately lead to cell wall synthesis and storage product accumulation in seeds, in particular in cereal caryopses are not fully understood.…”

Section: Introductionmentioning

confidence: 99%

SWEET11 and 15 as key players in seed filling in rice

et al. 2018

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SummaryDespite the relevance of seed-filling mechanisms for crop yield, we still have only a rudimentary understanding of the transport processes that supply the caryopsis with sugars. We hypothesized that SWEET sucrose transporters may play important roles in nutrient import pathways in the rice caryopsis.We used a combination of mRNA quantification, histochemical analyses, translational promoter-reporter fusions and analysis of knockout mutants created by genomic editing to evaluate the contribution of SWEET transporters to seed filling.In rice caryopses, SWEET11 and 15 had the highest mRNA levels and proteins localized to four key sites: all regions of the nucellus at early stages; the nucellar projection close to the dorsal vein; the nucellar epidermis that surrounds the endosperm; and the aleurone. ossweet11;15 double knockout lines accumulated starch in the pericarp, whereas caryopses did not contain a functional endosperm.Jointly, SWEET11 and 15 show all the hallmarks of being necessary for seed filling with sucrose efflux functions at the nucellar projection and a role in transfer across the nucellar epidermis/aleurone interface, delineating two major steps for apoplasmic seed filling, observations that are discussed in relation to observations made in rice and barley regarding the relative prevalence of these two potential import routes.

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“…In Arabidopsis, SWEET transporters are essential members in nectar secretion (Lin et al, 2014), phloem sugar loading (Chen et al, 2012), seed nutrient filling (Chen et al, 2015a), and pollen feeding and vacuolar fructose storage (Chardon et al, 2013;Klemens et al, 2013;Guo et al, 2014). In maize, ZmSWEET13a, b, and c are key components in apoplasmic phloem loading (Bezrutczyk et al, 2018); in rice, OzSWEET11 and 15 are crucial components in seed filling . Also, in Arabidopsis, AtSWEET13 and 14 are capable of transporting multiple forms of gibberellins, revealing an exciting plasticity of these transporters (Kanno et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

VvSWEET7 Is a Mono- and Disaccharide Transporter Up-Regulated in Response to Botrytis cinerea Infection in Grape Berries

et al. 2020

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The newly-identified SWEETs are high-capacity, low-affinity sugar transporters with important roles in numerous physiological mechanisms where sugar efflux is critical. SWEETs are desirable targets for manipulation by pathogens and their expression may be transcriptionally reprogrammed during infection. So far, few plant SWEET transporters have been functionally characterized, especially in grapevine. In this study, in the Botrytissusceptible variety "Trincadeira," we thoroughly analyzed modifications in the gene expression profile of key SWEET genes in Botrytis cinerea-infected grape berries. VvSWEET7 and VvSWEET15 are likely to play an important role during fruit development and Botrytis infection as they are strongly expressed at the green and mature stage, respectively, and were clearly up-regulated in response to infection. Also, B. cinerea infection down-regulated VvSWEET17a expression at the green stage, VvSWEET10 and VvSWEET17d expression at the veraison stage, and VvSWEET11 expression at the mature stage. VvSWEET7 was functionally characterized by heterologous expression in Saccharomyces cerevisiae as a low-affinity, high-capacity glucose and sucrose transporter with a K m of 15.42 mM for glucose and a K m of 40.08 mM for sucrose. VvSWEET7-GFP and VvSWEET15-GFP fusion proteins were transiently expressed in Nicotiana benthamiana epidermal cells and confocal microscopy allowed to observe that both proteins clearly localize to the plasma membrane. In sum, VvSWEETs transporters are important players in sugar mobilization during grape berry development and their expression is transcriptionally reprogrammed in response to Botrytis infection.

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Impaired phloem loading in zmsweet13a,b,c sucrose transporter triple knock‐out mutants in Zea mays

Cited by 146 publications

References 55 publications

The pomegranate (Punica granatum L.) draft genome dissects genetic divergence between soft‐ and hard‐seeded cultivars

The pomegranate (Punica granatum L.) draft genome dissects genetic divergence between soft‐ and hard‐seeded cultivars

SWEET11 and 15 as key players in seed filling in rice

VvSWEET7 Is a Mono- and Disaccharide Transporter Up-Regulated in Response to Botrytis cinerea Infection in Grape Berries

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