2021
DOI: 10.1038/s41419-021-03906-2
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Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels

Abstract: Embryonic stem cell (ESC) differentiation and somatic cell reprogramming are biological processes governed by antagonistic expression or repression of a largely common set of genes. Accurate regulation of gene expression is thus essential for both processes, and alterations in RNA processing are predicted to negatively affect both. We show that truncation of the DIDO gene alters RNA splicing and transcription termination in ESC and mouse embryo fibroblasts (MEF), which affects genes involved in both differenti… Show more

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Cited by 9 publications
(28 citation statements)
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“…We previously reported a role for the DIDO3 protein in mRNA metabolism in in vitro - cultured cells, as well as the early embryonic lethality of mutant mice lacking Dido1 exon 16 (E16) [ 23 , 24 ]. E16 encodes the carboxy-terminal half of DIDO3, to which its interactions with various components of the mRNA splicing and polyadenylation machinery have been mapped.…”
Section: Discussionmentioning
confidence: 99%
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“…We previously reported a role for the DIDO3 protein in mRNA metabolism in in vitro - cultured cells, as well as the early embryonic lethality of mutant mice lacking Dido1 exon 16 (E16) [ 23 , 24 ]. E16 encodes the carboxy-terminal half of DIDO3, to which its interactions with various components of the mRNA splicing and polyadenylation machinery have been mapped.…”
Section: Discussionmentioning
confidence: 99%
“…Here we show that tamoxifen treatment efficiently converted Dido1 floxE16 to ΔE16 deletion in adult mice expressing Cre-ERT2. This deletion was previously found to produce replication stress, chromosome segregation defects, and DNA damage in stem cells as well as in differentiated cultured cells, all of which presumably contribute to developmental arrest in mutant mouse embryos [ 18 , 24 ]. In contrast, adult E16 mice survive the deletion with relatively mild disease signs, the most visible of which is delayed growth; concomitantly, transaminase activities increase in serum.…”
Section: Discussionmentioning
confidence: 99%
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“…In brief, a mouse strain bearing an exon 16 anked by loxP sites was generated and interbred with Cre VAV1 -producing mice heterozygous for exon 16 deletion 55 . Hematopoietic populations were isolated from the offspring.…”
Section: Micementioning
confidence: 99%