Impaired survival of T helper cells in the absence of CD4

Strong, Julie; Wang, Qi; Killeen, Nigel

doi:10.1073/pnas.051329698

Cited by 20 publications

(18 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increased apoptosis of CD4 lineage cells has been observed previously in Cd4 Ϫ/Ϫ mice (26) and in mice with an MHC class II mutation that blocks CD4 binding (27). In Cd4 Ϫ/Ϫ mice, this apoptosis is partially counteracted by a marked increase in homeostatic T helper cell proliferation, with the net result of both effects being a reduced but nonetheless stable complement of CD4 lineage cells (26). To test whether late loss of CD4 (in lines 3778 and 3779) was also associated with increased apoptosis and homeostatic proliferation of CD4 Ϫ CD8 Ϫ T cells, the Cd4 lox/Ϫ dLck-hcre transgenic mice were fed BrdUrd continuously in their drinking water.…”

Section: Resultsmentioning

confidence: 99%

Homeostatic Competition Among T Cells Revealed by Conditional Inactivation of the Mouse Cd4 Gene

Wang

Strong

Killeen

2001

The Journal of Experimental Medicine

Self Cite

View full text Add to dashboard Cite

Absence of CD4 impairs the efficiency of T cell receptor (TCR) signaling in response to major histocompatibility complex (MHC) class II–presented peptides. Here we use mice carrying a conditional Cd4 allele to study the consequences of impaired TCR signaling after the completion of thymocyte development. We show that loss of CD4 decreases the steady-state proliferation of T cells as monitored by in vivo labeling with bromo-deoxyuridine. Moreover, T cells lacking CD4 compete poorly with CD4-expressing T cells during proliferative expansion after transfer into lymphopenic recipients. The data suggest that T cells compete with one another during homeostatic proliferation, and indicate that the basis of this competition is TCR signaling.

show abstract

Section: Resultsmentioning

confidence: 99%

Homeostatic Competition Among T Cells Revealed by Conditional Inactivation of the Mouse Cd4 Gene

Wang

Strong

Killeen

2001

The Journal of Experimental Medicine

Self Cite

View full text Add to dashboard Cite

show abstract

“…Inefficient recognition of low-affinity ligands by TCRs in the absence of CD4 provides a potential explanation for the observation that the survival and homeostatic expansion of CD4 lineage cells is impaired in Cd4 Ϫ/Ϫ mice (37,67). Several studies have pointed to a necessary role for ligand-dependent TCR signaling in the proliferation of T cells that occurs when they are transferred into T lymphopenic environments (68 -73).…”

Section: Despite Abnormal Selection Of Tcrs In Cd4mentioning

confidence: 99%

CD4 Promotes Breadth in the TCR Repertoire

Wang

Malherbe

Zhang

et al. 2001

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

A diverse population of MHC class II-restricted CD4 lineage T cells develops in mice that lack expression of the CD4 molecule. In this study, we show that the TCR repertoire selected in the absence of CD4 is distinct, but still overlapping in its properties with that selected in the presence of CD4. Immunization of mice lacking CD4 caused the clonal expansion of T cells that showed less breadth in the range of Ag-binding properties exhibited by their TCRs. Specifically, the CD4-deficient Ag-specific TCR repertoire was depleted of TCRs that demonstrated low-affinity binding to their ligands. The data thus suggest a key role for CD4 in broadening the TCR repertoire by potentiating productive TCR signaling and clonal expansion in response to the engagement of low-affinity antigenic ligands.

show abstract

“…The immune function will be disordered and thereafter cause diseases. If the amount and function of CD 4 + or CD 8 + T cells are abnormal, then the immune function of chickens will have close relations with the amount of CD 4 + and CD 8 + T cells and their proportion (Strong et al, 2001;Kamalavenkatesh et al, 2005). Many studies have shown that Radix Astragalus or Astragalus polysaccharide (APS) can increase the concentrations of CD 4 + lymphocytes and the CD 4 + /CD 8 + ratio, and decrease the concentrations of CD 8 + lymphocytes Yuan et al, 2005;Cai et al, 2006).…”

Section: Discussionmentioning

confidence: 97%

Effect of a Chinese herbal formula Astragalus immunomodulator on immune function of chickens

Zhang

Jiang

et al. 2009

Front. Agric. China

View full text Add to dashboard Cite

In order to investigate the immunomodulatory effects and the mechanism of a Chinese herbal medicine, Zengmiansan (Astragalus immunomodulator), on immune function of chickens, three hundred 1-day-old chickens were assigned randomly into 5 groups, i.e., the blank control group, the Chinese herbal medicine Zengmiansan (ZMS)-treated group, the cyclophosphamide group, the cyclophosphamide plus levamisole group, the cyclophosphamide plus ZMS group and the control group. All chickens were immunized with Lasota vaccine by nosedrip or eye-drop at the age of 6 days. Newcastle Disease (ND) antibody titers, growth indexes of the spleen, thymus and bursa of Fabricius, the concentrations of CD 4 + lymphocytes and CD 8 + lymphocytes in spleen, thymus and peripheral blood, and the apoptosis of splenocytes, thymocytes and bursa of Fabricius cells were observed at the ages of 14, 21, 28 and 35 days, respectively. Our results indicated that the NDV antibody titers of chickens in the Chinese herbal medicine ZMS-treated group at the ages of 14 and 21 days were significantly higher than that of the other groups (P < 0.01). The growth indexes of immune organs, the concentrations of CD 4 + lymphocytes and the ratio of CD 4 + /CD 8 + T lymphocytes of chickens in the Chinese herbal medicine ZMS-treated group at the ages of 14, 21 and 28 d were significantly higher than those of the other groups (P < 0.01). The apoptotic splenocytes, thymocytes and bursa of Fabricius cells of chickens in the ZMS-treated group were significantly lower than the other groups (P < 0.01) at the ages of 14, 21 and 28 days.

show abstract

Impaired survival of T helper cells in the absence of CD4

Cited by 20 publications

References 52 publications

Homeostatic Competition Among T Cells Revealed by Conditional Inactivation of the Mouse Cd4 Gene

Homeostatic Competition Among T Cells Revealed by Conditional Inactivation of the Mouse Cd4 Gene

CD4 Promotes Breadth in the TCR Repertoire

Effect of a Chinese herbal formula Astragalus immunomodulator on immune function of chickens

Contact Info

Product

Resources

About