2016
DOI: 10.1159/000445632
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Impaired VEGF Signaling in Lungs with Hypoplastic Esophageal Atresia and Effects on Branching Morphogenesis

Abstract: Background/Aims: Patients with esophageal atresia (EA) and tracheoesophageal fistula (TEF) often suffer chronic respiratory tract disease. We previously reported that primary lung maldevelopment caused by deficient branching of embryonal airways in experimental EA-TEF was induced by Adriamycin. In this study, we investigated the Vascular endothelial growth factor (VEGF) pathway in the developing lung in an EA-TEF rat model. We further analyzed the effect of recombinant VEGF treatment in vitro on branching morp… Show more

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Cited by 4 publications
(1 citation statement)
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“…Total RNA was extracted from snap-frozen lungs by Trizol reagent (Invitrogen, Carlsbad, CA) following the manufacture's protocol, and the concentration was determined spectrophotometrically in duplicate (Nano Vue; GE Healthcare, Buckinghamshire, England). Reverse transcription and amplification were performed as previously described [ 16 ]. Amplification was performed with a LightCycler 480 SYBR Green I Master (Roche, Mannheim, Germany) in 20 μL of reaction solution with specific primers for target genes.…”
Section: Methodsmentioning
confidence: 99%
“…Total RNA was extracted from snap-frozen lungs by Trizol reagent (Invitrogen, Carlsbad, CA) following the manufacture's protocol, and the concentration was determined spectrophotometrically in duplicate (Nano Vue; GE Healthcare, Buckinghamshire, England). Reverse transcription and amplification were performed as previously described [ 16 ]. Amplification was performed with a LightCycler 480 SYBR Green I Master (Roche, Mannheim, Germany) in 20 μL of reaction solution with specific primers for target genes.…”
Section: Methodsmentioning
confidence: 99%