Impairment of Alveolar Macrophage Phagocytosis by Ultrafine Particles

Renwick, Louise; Donaldson, Kenneth; Clouter, Anna

doi:10.1006/taap.2001.9128

Cited by 265 publications

(128 citation statements)

References 48 publications

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“…However, activation of alveolar macrophages is strongly associated with inflammatory reactions and ROS production (Renwick et al, 2001;Bhatt et al, 2002;Wang et al, 2007). Also, MIP1a, secreted from rnTiO 2 burden alveolar macrophages, is possibly involved in the promotion of lung carcinogenesis (Xu et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of Toxic Effects of Anatase and Rutile Type Nanosized Titanium Dioxide Particles in vivo and in vitro

Numano

Xu²,

Futakuchi³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Asian Pac J Cancer Prev, 15 (2), 929-935 IntroductionThere are three mineral forms of natural titanium dioxide particles: rutile, anatase and brookite. Engineered anatase and rutile nanosized titanium dioxide particles (anTiO 2 and rnTiO 2 ) are being manufactured in large quantities worldwide and applied in many fields including material industry, electronic industry, commercial products and biosystems. Due to differences in crystal structure, anTiO 2 has better photocatalytic activity than rnTiO 2 (Kakinoki et al., 2004). Accordingly, anTiO 2 is mainly used in paints, such as surface painting of the walls and windows of buildings and vehicles, and photocatalytic systems, while rnTiO 2 is preferentially used in cosmetics, sunscreen and food additives.Large quantity production and widespread application of nTiO 2 have given rise to concern about its health and AbstractTwo types of nanosized titanium dioxide, anatase (anTiO 2 ) and rutile (rnTiO 2 ), are widely used in industry, commercial products and biosystems. TiO 2 has been evaluated as a Group 2B carcinogen. Previous reports indicated that anTiO 2 is less toxic than rnTiO 2 , however, under ultraviolet irradiation anTiO 2 is more toxic than rnTiO 2 in vitro because of differences in their crystal structures. In the present study, we compared the in vivo and in vitro toxic effects induced by anTiO 2 and rnTiO 2 . Female SD rats were treated with 500 mg/ml of anTiO 2 or rnTiO 2 suspensions by intra-pulmonary spraying 8 times over a two week period. In the lung, treatment with anTiO 2 or rnTiO 2 increased alveolar macrophage numbers and levels of 8-hydroxydeoxyguanosine (8-OHdG); these increases tended to be lower in the anTiO 2 treated group compared to the rnTiO 2 treated group. Expression of MIP1a mRNA and protein in lung tissues treated with anTiO 2 and rnTiO 2 was also significantly up-regulated, with MIP1a mRNA and protein expression significantly lower in the anTiO 2 group than in the rnTiO 2 group. In cell culture of primary alveolar macrophages (PAM) treated with anTiO 2 and rnTiO 2 , expression of MIP1a mRNA in the PAM and protein in the culture media was significantly higher than in control cultures. Similarly to the in vivo results, MIP1a mRNA and protein expression was significantly lower in the anTiO 2 treated cultures compared to the rnTiO 2 treated cultures. Furthermore, conditioned cell culture media from PAM cultures treated with anTiO 2 had less effect on A549 cell proliferation compared to conditioned media from cultures treated with rnTiO 2 . However, no significant difference was found in the toxicological effects on cell viability of ultra violet irradiated anTiO 2 and rnTiO 2 . In conclusion, our results indicate that anTiO 2 is less potent in induction of alveolar macrophage infiltration, 8-OHdG and MIP1a expression in the lung, and growth stimulation of A549 cells in vitro than rnTiO 2 .

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Section: Discussionmentioning

confidence: 99%

Comparative Study of Toxic Effects of Anatase and Rutile Type Nanosized Titanium Dioxide Particles in vivo and in vitro

Numano

Xu²,

Futakuchi³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…The size relationship observed in this study has been reported by several other researchers. 8,20,21 The Ag ion is a type of metal ion; metal ions, such as Mg 2+ , act as cofactors to enzymes that utilize nucleotides as substrates and synthesize or cleave DNA. 22,23 Also, Ag + is known to undergo strong covalent binding with DNA.…”

Section: Discussionmentioning

confidence: 99%

Effects of Nanometer Sized Silver Materials on Biological Toxicity During Zebrafish Embryogenesis

Yeo¹,

Kang

2008

Bulletin of the Korean Chemical Society

106

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Commercial nanometer sized silver is widely used for its antibacterial effect; however, nanoparticles may also have ecotoxicological effects after being discharged into water. Nanometer sized silver can flow into aquatic environments, where it can exert a variety of physiologically effects in living organisms, including fish. The present study aimed to investigate the effect of nanometer sized silver on the development of zebrafish embryos, analyze the properties of commercial nanometer sized silver and define the toxicity relationship between embryogenesis and hatched flies. The commercial nanometer sized silver was analyzed in the Ag + ion form. The hatch rate decreased in the nano-silver exposed groups (10 and 20 ppt); furthermore, the hatched flies had an abnormal notochord, weak heart beat, damaged eyes and curved tail. The expression of the Sel N1 gene decreased in the nano-silver exposed groups, and the catalase activities of the exposed groups increased relative to those in the control group. Therefore, the Ag + ions in commercial nanometer sized silver could accumulate in aquatic environments and seriously damage the development of zebrafish embryos.

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“…In lungs, UFPs are captured by alveolar macrophages and initiate inflammation, at least in part, by impairing alveolar macrophage phagocytosis and stimulating the release of inflammatory mediators from macrophages. 28,31,32 In the liver, however, the phagocytic activity of resident macrophages (Kupffer cells), ie, the kinetics of clearance of fluorescence-labeled circulating latex particles, was found to be comparable between the groups after infusion of either 5ϫ10 7 UFPs or vehicle, with 56Ϯ10% versus 58Ϯ1% moving particles at 1 minute, 38Ϯ7% versus 25Ϯ8% at 3 minutes, and 10Ϯ1% versus 17Ϯ4% at 5 minutes. This finding suggests that the accumulation of UFPs in the liver does not affect Kupffer cell function.…”

Section: Immunostaining For Fibrinogen Vwf and P-selectinmentioning

confidence: 99%

Ultrafine Particles Exert Prothrombotic but Not Inflammatory Effects on the Hepatic Microcirculation in Healthy Mice In Vivo

Khandoga¹,

Stampfl²,

Takenaka³

et al. 2004

Circulation

121

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Background-Air pollution episodes are strongly associated with increased cardiovascular morbidity and mortality. The effect of ultrafine particles (UFPs), when translocated after inhalation, on the microcirculation of extrapulmonary organs remains unclear. Methods and Results-In C57BL/6 mice, either carbon black UFPs (1ϫ10 7 and 5ϫ10 7 ) or vehicle was infused intra-arterially. Two hours after infusion, platelet-and leukocyte-endothelial cell interactions, sinusoidal perfusion, endothelial fibrin(ogen) deposition, and phagocytic activity of Kupffer cells were analyzed by intravital video fluorescence microscopy in the liver microvasculature. Expression of fibrin(ogen), von Willebrand factor (vWF), and P-selectin on hepatic endothelium was determined by immunostaining. Apoptotic cells were quantified in TUNELstained tissue sections. Application of UFPs caused significantly enhanced platelet accumulation on endothelium of postsinusoidal venules and sinusoids in healthy mice. UFP-induced platelet adhesion was not preceded by platelet rolling but was strongly associated with fibrin deposition and an increase in vWF expression on the endothelial surface. In contrast, inflammatory parameters such as the number of rolling/adherent leukocytes, P-selectin expression/ translocation, and the number of apoptotic cells were not elevated 2 hours after UFP exposure. In addition, UFPs did not affect sinusoidal perfusion and Kupffer cell function. Conclusions-UFPs

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Impairment of Alveolar Macrophage Phagocytosis by Ultrafine Particles

Cited by 265 publications

References 48 publications

Comparative Study of Toxic Effects of Anatase and Rutile Type Nanosized Titanium Dioxide Particles in vivo and in vitro

Comparative Study of Toxic Effects of Anatase and Rutile Type Nanosized Titanium Dioxide Particles in vivo and in vitro

Effects of Nanometer Sized Silver Materials on Biological Toxicity During Zebrafish Embryogenesis

Ultrafine Particles Exert Prothrombotic but Not Inflammatory Effects on the Hepatic Microcirculation in Healthy Mice In Vivo

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