Impairment of respiration and oxidative phosphorylation by redox cyclers 2-nitrosofluorene and menadione

Klöhn, Peter-Christian; Neumann, Hans‐Günter

doi:10.1016/s0009-2797(97)00052-5

Cited by 20 publications

(10 citation statements)

References 33 publications

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“…1B). To determine whether c-Abl and Arg interact in response to other inducers of oxidative stress, wild-type MEFs were treated with menadione, a redox-cycling agent that increases ROS generation (39). Like H 2 O 2 , treatment with 25 M menadione increased the formation of c-Abl⅐Arg complexes, whereas exposure to higher concentrations had less of an effect (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Thus, treatment with 10 to greater than 160 M H 2 O 2 was associated with increases in c-Abl⅐Arg heterodimers, whereas treatment with 640 M H 2 O 2 resulted in binding of c-Abl and Arg at a level found in control cells. The finding that menadione and TNF-␣ also induce the formation of c-Abl⅐Arg heterodimers is in concert with the effects of these agents on redox cycling and ROS generation (39,41,42). Thus, c-Abl and Arg form heterodimers in response to diverse agents that induce oxidative stress.…”

Section: Ros Induce C-abl⅐arg Heterodimers-tomentioning

confidence: 98%

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Functional Interaction between the c-Abl and Arg Protein-tyrosine Kinases in the Oxidative Stress Response

Cao

Leng

Li³

et al. 2003

Journal of Biological Chemistry

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Section: Methodsmentioning

confidence: 99%

Section: Ros Induce C-abl⅐arg Heterodimers-tomentioning

confidence: 98%

Functional Interaction between the c-Abl and Arg Protein-tyrosine Kinases in the Oxidative Stress Response

Cao

Leng

Li³

et al. 2003

Journal of Biological Chemistry

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“…The molecular mechanisms underlying hepatocarcinogenesis and the major factors responsible for initiation and promotion remain unclear. A metabolite of 2‐AAF, 2‐nitrosofluorene, that causes redox‐cycling by draining electrons from the mitochondrial respiratory chain may trigger apoptosis 5, 6. Bitsch et al7 suggest that cells stressed by toxic doses of 2‐AAF are eliminated by apoptosis and that regenerative proliferation occurs to replace these cells.…”

mentioning

confidence: 99%

Differentially expressed transcripts in neoplastic hepatic nodules and neonatal rat liver studied by cDNA microarray analysis

Tellgren

Wood

Flores‐Morales

et al. 2003

Intl Journal of Cancer

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The molecular mechanisms underlying hepatocarcinogenesis remain unclear. Wistar rats treated with 2-AAF develop hepatocarcinoma in histologically well-characterised stages. In our study, cDNA microarrays were used to measure the expression of 3,000 genes during the progression of liver carcinogenesis in persistent neoplastic nodules. Because tumours frequently revert into a more poorly differentiated phenotype, we also studied the expression of the same set of transcripts in neonatal rat liver. Approximately 2,000 transcripts gave a detectable signal in experiments comparing gene expression in nodules and control tissue. Approximately 8% of these were identified as differentially expressed in liver nodules. The differentially expressed genes fell into several categories with putative or demonstrated roles in signal transduction, metabolism, detoxification, cell-structure and transport. Many of the differentially expressed genes in nodules were not previously known to be regulated during liver carcinogenesis. A universal transcript profile for gene expression in hepatic liver nodules and neonatal liver has been created.

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“…It has also been shown that respiration could be restored in the rat liver mitochondrial electron transport chain that was blocked by rotenone by adding menadione. It was proposed that menadione was able to bypass the rotenone block at the complex I/ubiquinone interface and feed electrons back into the respiratory chain to allow the synthesis of ATP13. In addition Fain has shown that both menadione and vitamin K 5 increased respiration, glucose oxidation, lactate accumulation, and fatty acid synthesis14.…”

Section: Resultsmentioning

confidence: 99%

Electrochemical detection of intracellular and cell membrane redox systems in Saccharomyces cerevisiae

Rawson

Downard

Baronian

2014

Sci Rep

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Redox mediators can interact with eukaryote cells at a number of different cell locations. While cell membrane redox centres are easily accessible, the redox centres of catabolism are situated within the cytoplasm and mitochondria and can be difficult to access. We have systematically investigated the interaction of thirteen commonly used lipophilic and hydrophilic mediators with the yeast Saccharomyces cerevisiae. A double mediator system is used in which ferricyanide is the final electron acceptor (the reporter mediator). After incubation of cells with mediators, steady state voltammetry of the ferri/ferrocyanide redox couple allows quantitation of the amount of mediator reduced by the cells. The plateau current at 425 mV vs Ag/AgCl gives the analytical signal. The results show that five of the mediators interact with at least three different trans Plasma Membrane Electron Transport systems (tPMETs), and that four mediators cross the plasma membrane to interact with cytoplasmic and mitochondrial redox molecules. Four of the mediators inhibit electron transfer from S. cerevisiae. Catabolic inhibitors were used to locate the cellular source of electrons for three of the mediators.

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Impairment of respiration and oxidative phosphorylation by redox cyclers 2-nitrosofluorene and menadione

Cited by 20 publications

References 33 publications

Functional Interaction between the c-Abl and Arg Protein-tyrosine Kinases in the Oxidative Stress Response

Functional Interaction between the c-Abl and Arg Protein-tyrosine Kinases in the Oxidative Stress Response

Differentially expressed transcripts in neoplastic hepatic nodules and neonatal rat liver studied by cDNA microarray analysis

Electrochemical detection of intracellular and cell membrane redox systems in Saccharomyces cerevisiae

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