Impairment of the Staufen1-NS1 interaction reduces influenza viral replication

Lee, Jun-Han; Oh, Jun-Young; Pascua, Philippe Noriel Q.; Kim, Eung‐Gook; Choi, Young‐Ki; Kim, Hyong Kyu

doi:10.1016/j.bbrc.2011.09.042

Cited by 13 publications

(11 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of IP revealed that Stau1 55 ‐HA 3 co‐immunopurified with 3xFLAG‐NS1 in a partially RNase‐resistant manner (Fig. 1A, upper), consistent with previous report [6–8], indicating that NS1 interacts with Stau1. Efficient removal of RNAs by RNase A treatment was demonstrated by RT‐PCR of endogenous glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) mRNAs using α‐[ 32 P]‐dATP and specific oligonucleotides (Fig.…”

Section: Resultssupporting

confidence: 91%

“…As a consequence, SMD‐targeted mRNAs would be stabilized and translated. Considering the previous reports that NS1–Stau1 interaction is important for influenza viral replication [7,8], the stabilization of SMD substrates by NS1 would make cellular environment more favorable for viral replication. For the complete understanding on the molecular role of NS1 in SMD during viral infection, future studies should identify the exact SMD substrates, which are regulated by NS1 upon viral infection.…”

Section: Discussionmentioning

confidence: 97%

“…In particular, the interaction of NS1 with Stau1 has long been known [6]. More recently, this interaction has been implicated in viral replication [7,8]. However, the underlying molecular mechanism has so far been poorly characterized.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Non‐structural protein 1 of influenza viruses inhibits rapid mRNA degradation mediated by double‐stranded RNA‐binding protein, staufen1

et al. 2013

View full text Add to dashboard Cite

Edited by Hans-Dieter Klenk Keywords:Influenza virus NS1 Staufen1 Staufen1-mediated mRNA decay (SMD) a b s t r a c t Although non-structural protein 1 (NS1) of influenza viruses is not essential for virulence, this protein is involved in host-virus interactions, viral replication, and translation. In particular, NS1 is known to interact with the host protein, staufen1 (Stau1). This interaction is important for efficient viral replication. However, the underlying molecular mechanism by which NS1 influences the viral life cycle remains obscure. Here, we show using immunoprecipitation and artificial tethering that the N-terminus of NS1, NS1(1-73), interacts with Stau1, blocks the Stau1-Upf1 interaction, and consequently inhibits the efficiency of Stau1-mediated mRNA decay (SMD), but not nonsense-mediatedmRNA decay (NMD). The regulation of SMD efficiency by NS1 may contribute to building a more favorable cellular environment for viral replication. Structured summary of protein interactions:STAU1-55 physically interacts with UPF1 by anti tag-coimmunoprecipitation (View interaction) NS1 physically interacts with STAU1-55 by anti tag-coimmunoprecipitation (View interaction)

show abstract

Section: Resultssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Non‐structural protein 1 of influenza viruses inhibits rapid mRNA degradation mediated by double‐stranded RNA‐binding protein, staufen1

et al. 2013

View full text Add to dashboard Cite

show abstract

“…; Lee et al . ). APC‐armadillo repeat regions were amplified from the pCMV‐neo‐APC vector (Addgene, Cambridge, MA, USA; Cat# 16507) by PCR (hAPC‐ARM‐Xho‐S, hAPC‐ARM‐R1‐A; or hAPC‐ARM‐R1 (HA)‐S, hAPC‐ARM‐Xho‐A), and were inserted at the EcoR I/ Xho I sites of the pCS4‐3xHA vector (donated by Seok‐Cheol Bae, Chungbuk Nat'l University, Cheongju, Korea) for HA tagging.…”

Section: Methodsmentioning

confidence: 97%

“…GFP-DH-PH was amplified with PCR (GFP-Mlu-S, hAsef1-Pml-A) and inserted to the pSinRep5 vector. Construction of Stau1 and Stau2 expression vectors has been described elsewhere (Nam et al 2008;Lee et al 2011). APC-armadillo repeat regions were amplified from the pCMV-neo-APC vector (Addgene, Cambridge, MA, USA; Cat# 16507) by PCR (hAPC-ARM-Xho-S, hAPC-ARM-R1-A; or hAPC-ARM-R1 (HA)-S, hAPC-ARM-Xho-A), and were inserted at the EcoRI/XhoI sites of the pCS4-3xHA vector (donated by Seok-Cheol Bae, Chungbuk Nat'l University, Cheongju, Korea) for HA tagging.…”

Section: Plasmid Constructsmentioning

confidence: 99%

Adenomatous polyposis coli‐stimulated GEF 1 (Asef1) is a negative regulator of excitatory synaptic function

Lim

Yoo

et al. 2018

Journal of Neurochemistry

Self Cite

View full text Add to dashboard Cite

Guanine nucleotide exchange factors (GEFs) play important roles in many cellular processes, including regulation of the structural plasticity of dendritic spines. A GEF protein, adenomatous polyposis coli-stimulated GEF 1 (Asef1, ARHGEF4) is highly expressed in the nervous system. However, the function of Asef1 has not been investigated in neurons. Here, we present evidence showing that Asef1 negatively regulates the synaptic localization of postsynaptic density protein 95 (PSD-95) in the excitatory synapse by inhibiting Staufen-mediated synaptic localization of PSD-95. Accordingly, Asef1 expression impairs synaptic transmission in hippocampal cultured neurons. In addition, neuronal activity facilitates the dissociation of Asef1 from Staufen in a phosphoinositide 3 kinase (PI3K)-dependent manner. Taken together, our data reveal Asef1 functions as a negative regulator of synaptic localization of PSD-95 and synaptic transmission.

show abstract

Targeting of viral RNAs by Upf1-mediated RNA decay pathways

May

Simon

2021

Current Opinion in Virology

View full text Add to dashboard Cite

Impairment of the Staufen1-NS1 interaction reduces influenza viral replication

Cited by 13 publications

References 28 publications

Non‐structural protein 1 of influenza viruses inhibits rapid mRNA degradation mediated by double‐stranded RNA‐binding protein, staufen1

Non‐structural protein 1 of influenza viruses inhibits rapid mRNA degradation mediated by double‐stranded RNA‐binding protein, staufen1

Adenomatous polyposis coli‐stimulated GEF 1 (Asef1) is a negative regulator of excitatory synaptic function

Targeting of viral RNAs by Upf1-mediated RNA decay pathways

Contact Info

Product

Resources

About