Impedance analysis of supported lipid bilayer membranes: a scrutiny of different preparation techniques

Steinem, Claudia; Janshoff, Andreas; Ulrich, Wolf-Peter; Sieber, Manfred; Galla, Hans‐Joachim

doi:10.1016/0005-2736(95)00274-x

Cited by 308 publications

(250 citation statements)

References 28 publications

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“…EIS was performed with a dc voltage of 50 mV at frequency ranging from 10 5 to 10 −2 Hz. The Bode plot obtained at 0 V was fitted by an equivalent circuit [30] consisting of the solution resistance (R s ), monolayer resistance (R m ), and monolayer constant phase element (CPE) [Z CPE = (1/(Q(iω) n ))] to determine these parameters. In this study, a CPE was used instead of a capacitance to get a better agreement between the experimental and simulated curves by accounting for the inhomogeneity of the electrode surface [31,32].…”

Section: Electrochemical Measurements (Cv Ca and Eis)mentioning

confidence: 99%

Distance dependence of long‐range electron transfer through helical peptides

Kai

Takeda

Morita

et al. 2007

Journal of Peptide Science

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Helical peptides of 8mer, 16mer, and 24mer carrying a disulfide group at the N -terminal and a ferrocene moiety at the C-terminal were synthesized, and they were self-assembled on gold by a sulfur-gold linkage. Infrared reflection-absorption spectroscopy and ellipsometry confirmed that they formed a monolayer with upright orientation. Cyclic voltammetry showed that the electron transfer from the ferrocene moiety to gold occurred even with the longest 24mer peptide. Chronoamperometry and electrochemical impedance spectroscopy were carried out to determine the standard electron transfer rate constants. It was found that the dependence of the electron-transfer rates on the distance was significantly weak with the extension of the chain from 16mer to 24mer (decay constant β = 0.02-0.04). This dependence on distance cannot be explained by an electron tunneling mechanism even if increased hydrogen-bonding cooperativity or molecular dynamics is considered. It is thus concluded that this long-range electron transfer is operated by an electron hopping mechanism.

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Section: Electrochemical Measurements (Cv Ca and Eis)mentioning

confidence: 99%

Distance dependence of long‐range electron transfer through helical peptides

Kai

Takeda

Morita

et al. 2007

Journal of Peptide Science

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“…[9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] However, little is known about the impact of the solid support on physical properties of lipid bilayers attached to surfaces such as the main phase transition temperature or lateral mobility of the lipids. It is expected that phase transitions are strongly affected if not entirely suppressed by the various different supports ranging from glass, gold, silicon, mica to all kinds of functionalized surfaces.…”

Section: Introductionmentioning

confidence: 99%

Melting and interdigitation of microstructured solid supported membranes quantified by imaging ellipsometry

2008

Self Cite

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The phase transition of individually addressable microstructured lipid bilayers was investigated by means of noncontact imaging ellipsometry. Two-dimensional membrane compartments were created on silicon substrates by micromolding in capillaries and the phase transition of supported dimyristoylphosphadiylcholine ͑DMPC͒ and dipentadecoylphosphatidylcholine ͑DiC 15 PC͒ membranes was determined measuring area expansion and thickness of the bilayer as a function of temperature, ethanol concentration, and cholesterol content. Apart from measuring the thermotropic behavior of DMPC on glass slides and silicon wafers, the authors were able to visualize the reversible induction of an interdigitated phase by partitioning of ethanol into the microstructured lipid bilayers. Interdigitation induced by addition of ethanol was measured as a function of cholesterol content and shifts of the main phase transition temperature T M of microstructured DiC 15 PC were quantified as a function of ethanol concentration. They observed that cholesterol abolishes interdigitation at higher concentrations and found a biphasic behavior of T M as a function of ethanol concentration in good accordance to what is known from vesicles in solution.

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“…The capacitance of the monolayer, the second phospholipid and the whole bilayer can be calculated as described by Gallar. 21 In this work, the specific capacitance of this paint-freeze lipid/ HDM bilayer is 0.68 µF/cm 2 higher than that of the painted lipid membrane (0.46 µF/cm 2 ) discussed in the literature 22 , resulting from changes in the membrane structure, due to the exclusion of an organic solvent from the membrane at low temperature.…”

Section: Resultsmentioning

confidence: 54%

Paint-Freeze Method to Form Self-Assembled Alkanethiol/Phospholipid Bilayers on Gold

Jing

Wang

1998

ANAL. SCI.

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In resent years, supported phospholipid membranes (SPM) on solids give rise to ever-more interest for several reasons. In contrast to black lipid membranes (BLMs), whose lifetime is often shorter than a few hours, they have advantages of ease and reproducibility of preparation, long-term stability and the possibility for use as electrically conductive supports. -4They provide a natural environment for the immobilization of proteins, such as hormone receptors and antibodies, under nondenaturing conditions and in a well-defined orientation.5 Especially, they enable the application of several surface-sensitive techniques, such as fluorescence recovery after photobleaching (FRAP) 6 , total internal reflection fluorescence microscopy (TIRFM) 7 , surface plasma spectroscopy, Fourier-transform infrared 8 , nuclear magnetic resonance (NMR) 9 and scanning probe microscope. 10Supported phospholipid bilayers using a hydrophobic surface of alkanethiol self-assembled on gold as the first layer is one of the best models of SPM. Several approaches to use this hydrophobic surface for the formation of structurally supported phospholipid bilayers have been reported, including painted 11 , L-B films 12 and vesicles. 13,14 In our previous work 15 we presented for the first time a simple paint-freeze method for preparing solvent-free alkanethiol/phospholipid bilayers on a gold surface based on the idea of White that organic solvents can be frozen out of a membrane at low temperatures. We used cyclic voltammetry, chronoamperometry/chronocoulometry and a.c. impedance measurements to characterize the properties of the SPM produced by the paint-freeze method, and discussed the differences with another two kinds of SPM prepared by L-B and painted methods, and prepared highly sensitive Na +16 and K +17 sensors by incorporating monensin and valinomycin into the membrane, respectively. In this work we further characterized the SPM by cyclic voltammetry, a.c impedance and ATR-FTIR and calculated the lipid order parameter from the dichroic ratio, which was 0.67. It was consistent with a well-ordered lipid film in which the methylene groups have segmental flexibility and are disordered to a degree which is typical for a lipid bilayer in the liquid-crystalline phase. Such a supported membrane provides a useful way for studies in biophysics, physiology and electrochemistry. Experimental ChemicalsHexadecylmercaptane (HDM, 92%) was of technical grade (Aldrich, USA) and L-α-phosphatidylcholine (PC) was approximately 99% (TLC) (Sigma Chemical Co. USA). Ethanol was of analytical grade. Decane (≥ 99.5%, Tongji University Chemicals) was of chromatographic standard grade. All other chemicals were of analytical grade. All solutions were prepared with water purified by Milli-QII (18.2 M, Millipore, USA). Preparation of the samplesWe used two substrates in the experiment. One was a gold-disk electrode for the cyclic voltammogram and a.c impedance measurements; the other was a gold film on a glass slide for infrared measurements. The gold electrode was c...

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Impedance analysis of supported lipid bilayer membranes: a scrutiny of different preparation techniques

Cited by 308 publications

References 28 publications

Distance dependence of long‐range electron transfer through helical peptides

Distance dependence of long‐range electron transfer through helical peptides

Melting and interdigitation of microstructured solid supported membranes quantified by imaging ellipsometry

Paint-Freeze Method to Form Self-Assembled Alkanethiol/Phospholipid Bilayers on Gold

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