2016
DOI: 10.1149/2.0301605jes
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Impedance Biosensor Incorporating a Carboxylate-Terminated Bidentate Thiol for Antibody Immobilization

Abstract: An impedance biosensor is reported that employs the bidentate thiol, 16- The antibody-coated electrodes retained activity towards Ara h1 for 10 and 20 days of regeneration of the monodentate-and BMPHA-coated Au electrodes, respectively, illustrating the superior stability of protein films atop the BMPHA bidentate thiol.3

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Cited by 13 publications
(13 citation statements)
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“…In both cases, the detection limit for the direct assay (1‐3x10 −4 M) is dramatically reduced by using a sandwich assay (1–5×10 −6 M). Due to such variations between sensor electrodes, impedance biosensors must be calibrated before usage, as recently demonstrated by repeated antibody recycling using 200 mM KSCN as a chaotropic agent .…”
Section: Resultsmentioning
confidence: 99%
“…In both cases, the detection limit for the direct assay (1‐3x10 −4 M) is dramatically reduced by using a sandwich assay (1–5×10 −6 M). Due to such variations between sensor electrodes, impedance biosensors must be calibrated before usage, as recently demonstrated by repeated antibody recycling using 200 mM KSCN as a chaotropic agent .…”
Section: Resultsmentioning
confidence: 99%
“…Further advancements are envisaged through the application of superior surface modification methods, such as the use of bidendate thiol 16-[3,5-bis(mercaptomethyl)phenoxy]-hexadecanoic acid (BMPHA), the application of proper solutions to stabilize the capture ligands, and the application of AC electrokinetic (ACEK) microfluidics platforms and ACEK microflow mechanisms, such as AC electroosmosis (ACEO) and AC electrothermal (ACET) effects. Furthermore, an increase in the limit of detection may be achieved by combining the biosensor methods with immunoseparation of bacteria from larger volumes [29,30].…”
Section: Discussionmentioning
confidence: 99%
“…We recently reported a standard deviation of~16 % across five different antibody-coated sensor electrodes for impe-dance detection of Listeria monocytogenes [31]. This adds complexity for antibody-based biosensors, since calibration requires repeated protein unfolding and refolding, [32,33].…”
Section: Direct Impedance Immunoassay Of 3-pbamentioning
confidence: 99%
“…We recently reported a standard deviation of~16 % across five different antibody-coated sensor electrodes for impe-dance detection of Listeria monocytogenes [31]. This adds complexity for antibody-based biosensors, since calibration requires repeated protein unfolding and refolding, 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 which can be accomplished using a mild chaotropic reagent such as 200 mM KSCN [32,33].…”
Section: Direct Impedance Immunoassay Of 3-pbamentioning
confidence: 99%