Impedance Characterization of Chitosan Cytotoxicity to MCF-7 Breast Cancer Cells Using a Multidisc Indium Tin Oxide Microelectrode Array

Abstract

Chitosan is an amino polysaccharide with possible biomedical applications, for example, in bandage materials or as an antibacterial agent or a cytotoxic agent for tumor and cancer cells. In this study, the cytotoxic effect of chitosan (CTSN-P) on MCF-7 breast cancer cells was monitored in a nondestructive and real-time manner by electrical cell-substrate impedance sensing with a fabricated multidisc indium tin oxide electrode array. The electrical impedance characteristics of cell growth on the multidisc electrode were analyzed by equivalent electric circuit modeling. Application of CTSN-P caused deterioration of viability of cells on the electrode substrate and yielded a CTSN-P concentration-dependent decrease in impedance. The 50% cytotoxicity concentrations in a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and impedimetric assay were 1266.60 and 543.17 μg/mL, respectively. Thus, impedance measurement is suitable for detecting low-dose effects of CTSN-P on the behavior or morphology of live cells.

Extending In-Plane Impedance Measurements from 2D to 3D Cultures: Design Considerations

“…Research using small working electrodes and interdigitated electrodes has shown that it is possible to monitor cells in two dimensional (2D) cultures using this technique. While small working electrodes have been used to monitor confluent cell cultures challenged with drugs, interdigitated working and reference electrodes have been shown to be more effective at monitoring cell proliferation across the well surface over time [ 7 , 10 , 25 , 30 ]. This highlights the importance of electrode configuration for monitoring cell cultures and the differences in their potential applications.…”

Four electrode-based impedimetric biosensors for evaluating cytotoxicity of tamoxifen on cervical cancer cells

“…At lower concentrations (12.5 mM) of the drug, cells are also in normal expanded morphology; however, at higher drug concentrations (37.5 mM) a large number of cells are rounded up and detached from the gold surface due to cell death as described in previous literature. 31,[47][48][49][50][51] Kustermann et al reported that a rise in impedance reveals cell proliferation however, a reduction can be reected as an insignia of cytotoxicity leading to death and detachment from the electrode surface. 47 Similarly, Wei et al also reported that with the increase of drug doses of doxorubicin, the cell death enhances which leads to the detachment of cells from the planer electrode areas.…”

Polymer-Based Biomaterials: An Emerging Electrochemical Sensor

“…It is examined that every matrix has its benefits and drawbacks; therefore, it is of great interest to develop new efficient novel matrices. Due to the presence of plentiful -NH 2 and -OH functional groups, it can easily react with bioactive molecules that results in a superior fabricating substrate for the covalent immobilization by means of covalent binding or electrostatic interactions (Park et al 2018). It demonstrates good quality adhesion characteristics in one of the study where it has been investigated for the development and adherence of well SNPs in the matrix of MWCNTs.…”