Impedance detection integrated with dielectrophoresis enrichment platform for lung circulating tumor cells in a microfluidic channel

Nguyen, Ngoc-Viet; Jen, Chun-Ping

doi:10.1016/j.bios.2018.08.059

Cited by 72 publications

(49 citation statements)

References 31 publications

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“…Figure 1 sketches the design of the proposed microchip, including a glass substrate, a gold electrode structure, and a straight microfluidic channel layer. The device was fabricated via soft photolithography and casting and molding techniques, as detailed in our previous publications [36,37]. The simple and sensitive microelectrode configuration patterned on the glass substrate was utilized to confirm the immobilization steps in the channel.…”

Section: Methodsmentioning

confidence: 99%

Selective Detection of Human Lung Adenocarcinoma Cells Based on the Aptamer-Conjugated Self-Assembled Monolayer of Gold Nanoparticles

Nguyen

Jen

2019

Micromachines

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This study established a microfluidic chip for the capture of A549 human lung circulating tumor cells via the aptamer-conjugated self-assembled monolayer (SAM) of gold nanoparticles (AuNPs) in the channel. AuNPs are among the most attractive nanomaterials for the signal enhancement of biosensors owing to their unique chemical, physical, and mechanical properties. The microchip was fabricated using soft photolithography and casting and molding techniques. A self-assembly method was designed to attach AuNPs, cell-specific aptamers, and target cells onto the desired area (i.e., SAM area). In this study, the gold microelectrode configuration was characterized by fluorescence microscopy and impedance measurements to confirm the important modification steps. Subsequently, several investigations with the proposed assay were conducted with different cell samples to determine the specific binding ability of the device for A549 adenocarcinoma cancer cells. This work has ensured a simple, convenient, selective, and sensitive approach for the development of biosensors for lung cancer detection during the early stages.

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Section: Methodsmentioning

confidence: 99%

Selective Detection of Human Lung Adenocarcinoma Cells Based on the Aptamer-Conjugated Self-Assembled Monolayer of Gold Nanoparticles

Nguyen

Jen

2019

Micromachines

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“…In recent years, electrical impedance-based approaches have been gaining much attention in biosensor research [25,26,27]. This type of sensor has many advantages, such as simplicity, miniaturizability, fast analysis, sensitive response, low cost, and suitability for integrated microsystems [28,29,30].…”

Section: Introductionmentioning

confidence: 99%

An Aptamer-Based Capacitive Sensing Platform for Specific Detection of Lung Carcinoma Cells in the Microfluidic Chip

et al. 2018

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Improvement of methods for reliable and early diagnosis of the cellular diseases is necessary. A biological selectivity probe, such as an aptamer, is one of the candidate recognition layers that can be used to detect important biomolecules. Lung cancer is currently a typical cause of cancer-related deaths. In this work, an electrical sensing platform is built based on amine-terminated aptamer modified-gold electrodes for the specific, label-free detection of a human lung carcinoma cell line (A549). The microdevice, that includes a coplanar electrodes configuration and a simple microfluidic channel on a glass substrate, is fabricated using standard photolithography and cast molding techniques. A procedure of self-assembly onto the gold surface is proposed. Optical microscope observations and electrical impedance spectroscopy measurements confirm that the fabricated microchip can specifically and effectively identify A549 cells. In the experiments, the capacitance element that is dominant in the change of the impedance is calculated at the appropriate frequency for evaluation of the sensitivity of the biosensor. Therefore, a simple, inexpensive, biocompatible, and selective biosensor that has the potential to detect early-stage lung cancer would be developed.

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“…Target enrichment and sensing mechanism. For trace particle detection in liquid, the pre-concentration of target particles is always crucial to a successful test [33][34][35]. Compared with most techniques for pre-concentration needing an extra incubation or equipment, AC electrokinetic (ACEK) effects can manipulate nanoparticles efficiently to realize target enrichment rapidly without extra processes or devices [36][37][38].…”

Section: Methodsmentioning

confidence: 99%

Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

Qi¹,

Zhang

Cheng³

et al. 2020

Preprint

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Impedance detection integrated with dielectrophoresis enrichment platform for lung circulating tumor cells in a microfluidic channel

Cited by 72 publications

References 31 publications

Selective Detection of Human Lung Adenocarcinoma Cells Based on the Aptamer-Conjugated Self-Assembled Monolayer of Gold Nanoparticles

Selective Detection of Human Lung Adenocarcinoma Cells Based on the Aptamer-Conjugated Self-Assembled Monolayer of Gold Nanoparticles

An Aptamer-Based Capacitive Sensing Platform for Specific Detection of Lung Carcinoma Cells in the Microfluidic Chip

Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

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