Impedance sensing of bladder cancer cells based on a single-cell-based DEP microchip

Chuang, Cheng-Hsin; Wei, Ching-Hua; Hsu, Yi-Chu; Huang, Hung-Fu; Hsiao, Fei‐Bin

doi:10.1109/icsens.2009.5398183

Cited by 7 publications

(4 citation statements)

References 7 publications

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“…In 2007, Zhang et al [ 5 ] proposed a different design for impedance measurement with single-cell resolution; he separated the electrodes for individual purposes of DEP trapping and impedance measurement; thus, long-term monitoring of dynamic process of endothelin-1-induced cardiomyocyte hypertrophy could be achieved. In the meanwhile, Chuang [ 6 , 7 ] demonstrated a DEP chip with multilayer electrodes and a micro-cavity array for trapping single cells in a micro-cavity by negative DEP force and sequentially sensing their impedance. In Chuang’s work, the impedance measurement of trapped cells in a micro-cavity did not have to apply DEP force due to the enhancement of the positioning and immobilization by the microstructure effects.…”

Section: Introductionmentioning

confidence: 99%

System-Level Biochip for Impedance Sensing and Programmable Manipulation of Bladder Cancer Cells

Chuang

Huang

2011

Sensors

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This paper develops a dielectrophoretic (DEP) chip with multi-layer electrodes and a micro-cavity array for programmable manipulations of cells and impedance measurement. The DEP chip consists of an ITO top electrode, flow chamber, middle electrode on an SU-8 surface, micro-cavity arrays of SU-8 and distributed electrodes at the bottom of the micro-cavity. Impedance sensing of single cells could be performed as follows: firstly, cells were trapped in a micro-cavity array by negative DEP force provided by top and middle electrodes; then, the impedance measurement for discrimination of different stage of bladder cancer cells was accomplished by the middle and bottom electrodes. After impedance sensing, the individual releasing of trapped cells was achieved by negative DEP force using the top and bottom electrodes in order to collect the identified cells once more. Both cell manipulations and impedance measurement had been integrated within a system controlled by a PC-based LabVIEW program. In the experiments, two different stages of bladder cancer cell lines (grade III: T24 and grade II: TSGH8301) were utilized for the demonstration of programmable manipulation and impedance sensing; as the results show, the lower-grade bladder cancer cells (TSGH8301) possess higher impedance than the higher-grade ones (T24). In general, the multi-step manipulations of cells can be easily programmed by controlling the electrical signal in our design, which provides an excellent platform technology for lab-on-a-chip (LOC) or a micro-total-analysis-system (Micro TAS).

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Section: Introductionmentioning

confidence: 99%

System-Level Biochip for Impedance Sensing and Programmable Manipulation of Bladder Cancer Cells

Chuang

Huang

2011

Sensors

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“…Cells might potentially be described by their electrical response across a very small frequency range as indicated by their impedance values. Thus, EIS may be used to classify cancer cells [74].…”

Section: Analyzing the Electrical Properties Of Different Cancer Cellsmentioning

confidence: 99%

Modifying the electrical, optical, and magnetic properties of cancer cells: A comprehensive approach for cancer management

Sharma,

Malviya

2024

Medicine Advances

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Despite the recent successes of cancer therapy, numerous obstacles remain. The medical community of the 16th century believed that magnets might be used to cure or prevent illness. However, this concept has only recently been put into practice in the treatment of cancer. Cancers vary greatly not only at the patient, tissue, and cellular levels but also at the molecular level. Because of this multiscale heterogeneity, effective treatments that not only differentiate between cancerous and healthy tissues but also target a wide variety of tumor subclones are difficult to develop. Most treatments either take advantage of a specific biological characteristic shared by cancer cells (e.g., their propensity for rapid division) or indiscriminately eradicate every cell in an area of interest. In this article, we review the physical, chemical, electrical, optical, and magnetic properties of cancer cells, before discussing how these properties may be modulated by current and future cancer therapies.

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“…The cells transfer to other parts of the body through the circulatory system and become circulating tumor cells. At the onset of the disease, these cells are extremely difficult to detect from blood or biopsy samples, and metastatic tumor cells show a unique pattern of activity [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Design of a Lab-On-Chip for Cancer Cell Detection through Impedance and Photoelectrochemical Response Analysis

et al. 2022

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In this study, a biochip was fabricated using a light-absorbing layer of a silicon solar element combined with serrated, interdigitated electrodes and used to identify four different types of cancer cells: CE81T esophageal cancer, OE21 esophageal cancer, A549 lung adenocarcinoma, and TSGH-8301 bladder cancer cells. A string of pearls was formed from dielectrophoretic aggregated cancer cells because of the serrated interdigitated electrodes. Thus, cancer cells were identified in different parts, and electron–hole pairs were separated by photo-excited carriers through the light-absorbing layer of the solar element. The concentration catalysis mechanism of GSH and GSSG was used to conduct photocurrent response and identification, which provides the fast, label-free measurement of cancer cells. The total time taken for this analysis was 13 min. Changes in the impedance value and photocurrent response of each cancer cell were linearly related to the number of cells, and the slope of the admittance value was used to distinguish the location of the cancerous lesion, the slope of the photocurrent response, and the severity of the cancerous lesion. The results show that the number of cancerous cells was directly proportional to the admittance value and the photocurrent response for all four different types of cancer cells. Additionally, different types of cancer cells could easily be differentiated using the slope value of the photocurrent response and the admittance value.

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Impedance sensing of bladder cancer cells based on a single-cell-based DEP microchip

Cited by 7 publications

References 7 publications

System-Level Biochip for Impedance Sensing and Programmable Manipulation of Bladder Cancer Cells

System-Level Biochip for Impedance Sensing and Programmable Manipulation of Bladder Cancer Cells

Modifying the electrical, optical, and magnetic properties of cancer cells: A comprehensive approach for cancer management

Design of a Lab-On-Chip for Cancer Cell Detection through Impedance and Photoelectrochemical Response Analysis

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