Impedance spectroscopic detection of binding and reactions in acid-labile dielectric polymers for biosensor applications

Abstract

We synthesized previously unreported copolymers with cleavable acid-labile side chains for use as electrochemical sensing layers in order to demonstrate a novel architecture for a one-step immunosensor. This one-step system is in contrast to most antigen-capture signal amplification methods that involve complicated secondary labeling techniques, or require the addition of redox probes to achieve a sensing response. A series of novel copolymers composed of various trityl-containing monomers were synthesized and characterized to determine their dielectric properties. Results indicate that the thin films of these polymers are stable in water, but some begin to degrade under acidic conditions or upon antigen binding, causing observable changes in the phase angle.

Self-assembling biomolecules for biosensor applications

“…Impedance is essentially a measure of the resistance to alternating current (AC) flow in an electrical circuit [ 120 ]. Within an impedance biosensor, a change in impedance is caused by a change in the dielectric properties of the solution due to the presence of the analyte [ 121 ]. This change in impedance can be quantified by applying an AC signal to the biosensor and measuring the resulting changes in current and voltage across the electrodes [ 122 ].…”

Process Model Development of Lithium-ion Batteries — An Electrochemical Impedance Spectroscopy Simulation

“…Electrochemical impedance spectroscopy (EIS) is a broadly used noninvasive technique for variety of systems including batteries [1][2][3][4][5], fuel cells [6][7][8][9], corrosion detecting [10][11][12][13][14], biosensors [15][16][17][18][19], and so on [20].…”

The Process Model Development of Lithium-ion Batteries: An Electrochemical Impedance Spectroscopy Simulation

“…Electrochemical impedance spectroscopy (EIS) is a broadly used noninvasive technique for variety of systems including batteries [1][2][3][4][5], fuel cells [6][7][8][9], corrosion detecting [10][11][12][13][14], biosensors [15][16][17][18][19], and so on [20]. Figure 1.…”