Impedance spectroscopy: An accurate method of differentiating between viable and ischaemic or infarcted muscle tissue

Abstract

The object of the paper is to present results that show that impedance spectroscopy is an accurate method of assessing the condition of muscle tissue. Specimens of muscle tissue were excised from 36 Atlantic salmon and subjected to impedance spectroscopy measurements made at intervals during an 8h period of ischaemia and necrosis. These measurements were conducted for three different temperatures and for both the longitudinal and the transverse orientations of the muscle fibres. The specimens were also subjected to ATP, pH and visco-elastic measurement and analysis to establish the degree of correlation between changes in these quantities and impedance spectroscopy parameters due to ischaemia. It was concluded that the mean relaxation time, tau(c) was the impedance spectroscopy parameter that best described the changes taking place in the muscle tissue during the post-mortem period, decreasing by 60-76% during the 8h. This was the case for all three temperatures and for both orientations. Furthermore, the muscle tissue changes due to ischaemia, as reflected in the decrease in the mean relaxation time tau(c), were highly correlated with changes in the tissue ATP, pH and dynamic shear storage modulus G'.

Altered muscle electrical tissue properties in a mouse model of premature aging

“…Our study has some limitations. First, because tissue was only measured shortly after death, postmortem ischemia could have altered the impedance data as compared with living muscle . However, our previous work in both humans and mice support that these impedance alterations are also readily detectable using surface EIM methods .…”

Time-lapse electrical impedance spectroscopy for monitoring the cell cycle of single immobilized S. pombe cells

“…At higher frequencies, above 1 MHz information related to the cell membrane (open ion channels, membrane polarization) and information on intracellular compartments, such as cytoplasm, vacuoles, and the cell nucleus, can be gained. Impedance spectroscopy can also be used to detect cell motion 12 13 or cellular mechanical (muscle cells) 14 and electrical (cardiac cells) activity 15 .…”

Caracterización y validación clínica de espectros de impedancia de la mucosa gástrica

“…El comportamiento de las regiones de dispersión después de un periodo de isquemia ha sido reportado por Schwan [55]. Varios investigadores han observado recientemente la relación entre isquemia tisular y las mediciones de impedancia tisular [23,61,62,63], aunque se han reportado estudios previos de isquemia inducida y de tejido postmortem [64]. Resumiendo los trabajos de Schwan [55] y Ristic [64] sobre los cambios en las regiones de dispersión asociados a isquemia, se concluye: Durante la isquemia hay un movimiento de fluido del espacio extracelular al intracelular.…”