Impedance Spectroscopy Technique for DNA Hybridization

Abstract

Abstract:The development of biosensors for detection and identification of DNA sequences by hybridization may reduce assay time and allow direct quantitation of the target. This article describes the use of impedance spectroscopy technique for digoxigeninthiol-labeled ssDNA probe immobilization, anti-digoxigenin binding and targeted DNA hybridization. The analysis of the impedance spectra in terms of equivalents circuits of the gold/electrolyte interface and gold/digoxigenin-thiol-labeled/electrolyte interface is discussed. The DNA hybridization shows a variation in the impedance spectra.

An impedance spectroscopy method for the detection and evaluation of Babesia bovis antibodies in cattle

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Atrazine analysis using an impedimetric immunosensor based on mixed biotinylated self-assembled monolayer

“…The constant phase element reflects an inhomogeneity and defect area of the layer [22][23][24][25][26][27][28][29]. The extent of the deviation from the Randles and Ershler model is controlled by the parameter n in Eq.…”

DNA microdevice for electrochemical detection of Escherichia coli 0157:H7 molecular markers

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