Impedance Study of Dopamine Effects after Application on 2D and 3D Neuroblastoma Cell Cultures Developed on a 3D-Printed Well

Abstract

In this work, the assessment of the interactions of a bioactive substance applied to immobilized cells in either a two-dimensional (2D) or three-dimensional (3D) arrangement mimicking in vivo tissue conditions is presented. In particular, dopamine (DA) was selected as a stimulant for the implementation of an impedance analysis with a specific type of neural cells (murine neuroblastoma). The aim of this study was the extraction of calibration curves at various frequencies with different known dopamine concentrations for the description of the behavior of dopamine applied to 2D and 3D cell cultures. The results present the evaluation of the mean impedance value for each immobilization technique in each frequency. The differential responses showed the importance of the impedance when frequency is applied in both 2D and 3D immobilization cases. More specifically, in 2D immobilization matrix impedance shows higher values in comparison with the 3D cell culture. Additionally, in the 3D case, the impedance decreases with increasing concentration, while in the 2D case, an opposite behavior was observed.

3D-Printed Sensors and Actuators in Cell Culture and Tissue Engineering: Framework and Research Challenges

“…The system showed a good correlation with the conventional imaging methods for monitoring 3D cell viability. In another study [ 29 ], two silver (Ag) electrodes were embedded in a 3D-printed PETG well for impedance analysis measurements on immobilized N2a mouse neuroblastoma cells treated with dopamine. Two different cell culture systems were used, allowing either 2D or 3D cell growth (shown in Figure 2 ).…”

Impedance In Vitro Assessment for the Detection of Salmonella typhimurium Infection in Intestinal Human Cancer Cells

“…Our research group has previously employed this approach to characterize interactions between breast cancer cells and different chemotherapeutical agents [24,25] as well as neuronal cells and neurotransmitters [26]. In the present study, we compared the Salmonella infection progression in the human colon cancer cell line Caco-2 and the mucus-secreting HT29-MTX-E12, after treatment with a different bacterial multiplicity of infection (MOI) by assessing the alterations of frequencies recordings with impedance spectroscopy measurements.…”