Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening

Tertiş, Mihaela; Leva, Petrica Ionut; Bogdan, Diana; Suciu, Maria; Graur, Florin; Cristea, Cécilia

doi:10.1016/j.bios.2019.05.012

Cited by 121 publications

(76 citation statements)

References 56 publications

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“…In another study, IL-6 is pointed out as a biomarker for screening colorectal cancer. Instead of using the microelectrodes, the authors of this study employed the conventional glassy carbon electrode (GCE) ( Tertis et al, 2019 ). The electrode was modified with p-aminobenzoic acid, p-aminothiophenol and gold nanoparticles, which was further used to bind thiol terminated aptamers specific for IL-6.…”

Section: Inflammatory Biomarkers and Their Detection Platformsmentioning

confidence: 99%

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

Garg

Sharma

Singh

2021

Biosensors and Bioelectronics

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COVID-19 pandemic has affected everyone throughout the world and has resulted in the loss of lives of many souls. Due to the restless efforts of the researchers working hard day and night, some success has been gained for the detection of virus. As on date, the traditional polymerized chain reactions (PCR), lateral flow devices (LFID) and enzyme linked immunosorbent assays (ELISA) are being adapted for the detection of this deadly virus. However, a more exciting avenue is the detection of certain biomarkers associated with this viral infection which can be done by simply re-purposing our existing infrastructure. SARS-CoV-2 viral infection triggers various inflammatory, biochemical and hematological biomarkers. Because of the infection route that the virus follows, it causes significant inflammatory response. As a result, various inflammatory markers have been reported to be closely associated with this infection such as C-reactive proteins, interleukin-6, procalcitonin and ferritin. Sensing of these biomarkers can simultaneously help in understanding the illness level of the affected patient. Also, by monitoring these biomarkers, we can predict the viral infections in those patients who have low SARS-CoV-2 RNA and hence are missed by traditional tests. This can give more targets to the researchers and scientists, working in the area of drug development and provide better prognosis. In this review, we propose to highlight the conventional as well as the non-conventional methods for the detection of these inflammatory biomarkers which can act as a single platform of knowledge for the researchers and scientists working for the treatment of COVID-19.

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Section: Inflammatory Biomarkers and Their Detection Platformsmentioning

confidence: 99%

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

Garg

Sharma

Singh

2021

Biosensors and Bioelectronics

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“…An innovative format has been based on aptamers (Apt), which can be oligonucleotides with an extraordinarily high and specific affinity for the target [163]. Tertis et al [164] proposed a particular platform consisting of nanocomposites for the detection of interleukin-6 (IL-6) in human blood. The electrochemical biosensor was based on the modification of GCE with p-aminobenzoic acid, p-aminothiophenol, and AuNP, as shown in Figure 12.…”

Section: Electrochemical Biosensing Of Protein Biomarkers Of Crcmentioning

confidence: 99%

mentioning

confidence: 99%

Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers

et al. 2020

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The accurate determination of specific tumor markers associated with cancer with non-invasive or minimally invasive procedures is the most promising approach to improve the long-term survival of cancer patients and fight against the high incidence and mortality of this disease. Quantification of biomarkers at different stages of the disease can lead to an appropriate and instantaneous therapeutic action. In this context, the determination of biomarkers by electrochemical biosensors is at the forefront of cancer diagnosis research because of their unique features such as their versatility, fast response, accurate quantification, and amenability for multiplexing and miniaturization. In this review, after briefly discussing the relevant aspects and current challenges in the determination of colorectal tumor markers, it will critically summarize the development of electrochemical biosensors to date to this aim, highlighting the enormous potential of these devices to be incorporated into the clinical practice. Finally, it will focus on the remaining challenges and opportunities to bring electrochemical biosensors to the point-of-care testing.

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“…Several drugs have been produced to contrast IL-6 activity [ 18 ]. The detection of very small alterations of cytokine levels [ 19 ], and specifically of IL-6, could be helpful in the early recognition of an inflammatory state [ 20 , 21 , 22 , 23 , 24 ]. Biosensors may use aptamers, antibodies, and also antibody fragments (Fabs) as detection elements [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…Finally, we are interested in understanding whether the best-fitting arrangement of the receptor-ligand pair can be quantified. For this purpose, IL-6 offers an uncommonly wide set of different choices (receptors), between aptamers [ 20 , 21 , 28 ] and Fabs [ 22 , 23 ]. Aptamers are relatively young synthetic biomolecules [ 29 ] made of single strands of DNA or RNA selected in vitro to bind with high affinity a specific ligand (proteins, viruses, ions).…”

Section: Introductionmentioning

confidence: 99%

Biosensing Cytokine IL-6: A Comparative Analysis of Natural and Synthetic Receptors

2020

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Cytokines are a family of proteins which play a major role in the regulation of the immune system and the development of several diseases, from rheumatoid arthritis to cancer and, more recently, COVID-19. Therefore, many efforts are currently being developed to improve therapy and diagnosis, as well as to produce inhibitory drugs and biosensors for a rapid, minimally invasive, and effective detection. In this regard, even more efficient cytokine receptors are under investigation. In this paper we analyze a set of IL-6 cytokine receptors, investigating their topological features by means of a theoretical approach. Our results suggest a topological indicator that may help in the identification of those receptors having the highest complementarity with the protein, a feature expected to ensure a stable binding. Furthermore, we propose and discuss the use of these receptors in an idealized experimental setup.

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Impedimetric aptasensor for the label-free and selective detection of Interleukin-6 for colorectal cancer screening

Cited by 121 publications

References 56 publications

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

Advancement in biosensors for inflammatory biomarkers of SARS-CoV-2 during 2019–2020

Electrochemical Biosensors for Determination of Colorectal Tumor Biomarkers

Biosensing Cytokine IL-6: A Comparative Analysis of Natural and Synthetic Receptors

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