Impedimetric detection of Fumonisin B1 and its biointeraction with fsDNA

Kesici, Ece; Erdem, Arzum

doi:10.1016/j.ijbiomac.2019.08.024

Cited by 15 publications

(6 citation statements)

References 35 publications

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“…The third biosensor combined the modification of AuNPs with aptamers and 4-mercaptobenzoic acid as a Raman reporter, whose signal was reduced after target incubation through dehybridization from a cDNA-AuNP-(4-MBA) complex, with an LOD of 0.00002 µg/L [198]. The effect of the electrochemical interaction between FB1 and fish sperm doublestranded DNA was examined on the impedimetric detection with a pencil graphite electrode, which provides a promising biosensing technique with other DNA structures apart from aptamers [178]. Nevertheless, the addition of five FB1 concentrations did not portray differentiated responses; therefore, more optimization would be ideal for the application of this type of non-specific sequences.…”

Section: Not Specified Sequences and Alternative Methodsmentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida¹,

Gong²,

Goycoolea³

2020

Preprint

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Mycotoxin contamination is a current issue affecting several crops and processed products worldwide. Among the diverse mycotoxin group, fumonisin B1 (FB1) has become a relevant compound because of its adverse effects in the food chain. Conventional analytical methods previously proposed to quantify FB1 comprise LC-MS, HPLC-FLD and ELISA, while novel approaches integrate different sensing platforms and fluorescently labelled agents in combination with antibodies. Nevertheless, such methods could be expensive, time-consuming and require experience. Aptamers (ssDNA) are promising alternatives to overcome some of the drawbacks of conventional analytical methods, their high affinity through specific aptamer-target binding has been exploited in various designs attaining favorable limits of detection (LOD). So far, two aptamers specific to FB1 have been reported, and their modified and shortened sequences have been explored for a successful target quantification. In this critical review spanning the last eight years, we have conducted a systematic comparison based on principal component analysis of the aptamer-based techniques for FB1, compared with chromatographic, immunological and other analytical methods. We have also conducted an <i>in-silico</i> prediction of the folded structure of both aptamers under their reported conditions. The potential of aptasensors for the future development of highly sensitive FB1 testing methods is emphasized.

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Section: Not Specified Sequences and Alternative Methodsmentioning

confidence: 99%

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Mirón-Mérida¹,

Gong²,

Goycoolea³

2020

Preprint

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“…Biosensors based on electrochemical impedance spectroscopy as transduction mode [66], are appreciated for their sensitivity, selectivity, and portability [66,67,198]. Thus, Kesici and Erdem [198] achieved a detection limit for FUM of 3.69 ng mL −1 with an impedimetric biosensor.…”

Section: Comparative Overview and Future Perspectivementioning

confidence: 99%

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Pisoschi,

Iordache,

Stanca

et al. 2024

Chemosensors

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The need for performant analytical methodologies to assess mycotoxins is vital, given the negative health impact of these compounds. Biosensors are analytical devices that consist of a biological element for recognizing the analyte and a transducer, which translates the biorecognition event into a signal proportional to the analyte concentration. The biorecognition elements can be enzymes, antibodies, or DNA fragments. The modalities of detection can be optical, electrochemical, thermal, or mass-sensitive. These analytical tools represent viable alternatives to laborious, expensive traditional methods and are characterized by specificity given by the biorecognition element, sensitivity, fast response, portability, multi-modal detection, and the possibility of in situ application. The present paper focuses on a comprehensive view, enriched with a critical, comparative perspective on mycotoxin assay using biosensors. The use of different biorecognition elements and detection modes are discussed comparatively. Nanomaterials with optical and electrochemical features can be exploited in association with a variety of biorecognition elements. Analytical parameters are reviewed along with a broad range of applications.

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“…Exposure to mycotoxins can cause a range of adverse health effects, including liver and kidney damage, immune system suppression, gastrointestinal irritation, and even cancer. Mycotoxins are a significant concern for food safety, and efforts have been made to reduce their levels in food products through good agricultural and manufacturing practices, as well as by regular testing and monitoring [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Smartphone-Controlled Aptasensor for Voltammetric Detection of Patulin in Apple Juice

Erdem,

Senturk

2024

Sensors

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Patulin (PAT) is a mycotoxin that adversely affects the health of humans and animals. PAT can be particularly found in products such as apples and apple juice and can cause many health problems if consumed. Therefore, accurate and sensitive determination of PAT is very important for food quality and human and animal health. A voltammetric aptasensor was introduced in this study for PAT determination while measuring the changes at redox probe signal. The limit of detection (LOD) was found to be 0.18 pg/mL in the range of 1–104 pg/mL of PAT in buffer medium under optimum experimental conditions. The selectivity of the PAT aptasensor against ochratoxin A, fumonisin B1 and deoxynivalenol mycotoxins was examined and it was found that the aptasensor was very selective to PAT. PAT determination was performed in an apple juice medium for the first time by using a smartphone-integrated portable device, and accordingly, an LOD of 0.47 pg/mL was achieved in diluted apple juice medium. A recovery range of 91.24–93.47% was obtained for PAT detection.

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Impedimetric detection of Fumonisin B1 and its biointeraction with fsDNA

Cited by 15 publications

References 35 publications

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Aptamer-Based Detection of Fumonisin B1: A Systematic Comparison with Conventional and Other Novel Methods

Biosensors for Food Mycotoxin Determination: A Comparative and Critical Review

Smartphone-Controlled Aptasensor for Voltammetric Detection of Patulin in Apple Juice

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