Impedimetric DNA E-biosensor for multiplexed sensing of Escherichia coli and its virulent f17 strains

Rabti, Amal; Zayani, Riham; Meftah, Marwa; Salhi, Imed; Raouafi, Noureddine

doi:10.1007/s00604-020-04614-y

Cited by 16 publications

(9 citation statements)

References 32 publications

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“…The Nyquist plots demonstrated that the charge-transfer resistance of Ab−AuNPs/SPCE (R ct = 571.3 ± 2.9 Ω ( n = 3)) is lower than that of bare SPCE (R ct = 787.5 ± 2.4 Ω), due to the enhancement of the electro−active surface area and conductivity of the working electrode by the Ab−AuNPs conjugate. After incubation in F17A protein solution, the resistance further decreased (R ct = 469.1 ± 3.6 Ω), which is probably related to the attractive ionic interactions between the positively charged F17A protein layers and the negatively charged redox ions [ 21 ]. Subsequently, the R ct value increased (R ct = 591.5 ± 3.2 Ω) after incubation of HRP−Ab as the sandwich complex formation impede the diffusion of the redox probe toward the electrode surface to be oxidized or reduced.…”

Section: Resultsmentioning

confidence: 99%

“…General interest on biosensors using nanomaterials such as graphene [ 19 ], carbon nanotubes [ 20 ], gold nanoparticles [ 21 , 22 ], magnetic beads [ 23 ] and silicon nanowires [ 24 ] for protein detection and quantification applications is increasingly growing owing to their high sensitivity, specificity, and speed at lower costs. Andrei et al developed a surface-enhanced Raman spectroscopy (SERS)-based biosensor for the detection of uropathogenic E. coli strains containing type-1 fimbriae in urine [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…They used cetyltrimethyl ammonium bromide−stabilized gold nanorods, as SERS markers, and anti−fimbrial antibodies against the major pilin protein FimA as surface probes. Moreover, Rabti et al designed an impedimetric multiplexed DNA biosensor based on gold nanoparticle−modified screen-printed carbon electrode for simultaneous detection of E. coli (yaiO gene) and its virulent F17–positive variant [ 21 ]. However, development of biosensors for the detection of variant fimbriae expressed by pathogenic E. coli is still a necessity.…”

Section: Introductionmentioning

confidence: 99%

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Sandwich-Based Immunosensor for Dual-Mode Detection of Pathogenic F17–Positive Escherichia coli Strains

Salhi

Rabti

Dhehibi

et al. 2022

IJMS

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Bacterial diseases cause tremendous economic losses due to high morbidity and mortality in livestock animals. F17A protein, the major subunit of F17 fimbriae, is one of the most prevalent and crucial virulence factors among the pathogenic Escherichia coli (E. coli) isolated from diarrheic and septicemic animals of various species. Purification and detection of this protein is regarded as an interesting field of investigation due to its important role as a therapeutic target, such as vaccines, and as a diagnostic tool. In this context, polyclonal rabbit antibodies recognizing F17A protein (anti−F17A antibody) were developed and used for its detection. In fact, sandwich biosensor using anti−F17A/gold nanoparticles conjugates as capture probe and anti−F17A antibody labelled with horseradish peroxidase as signal amplification probe was developed for electrochemical and fluorescent detection of purified F17A protein and live F17–positive E. coli bacteria. Good specificity and sensitivity for detection of F17–positive E. coli strains were obtained. The dynamic range for the biosensor varies from 1 × 102 to 1 × 109 CFU·mL−1 (R2 = 0.998) and the detection limit (LOD) and the IC50 value were estimated to be 37 CFU·mL−1 and 75 CFU·mL−1, respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sandwich-Based Immunosensor for Dual-Mode Detection of Pathogenic F17–Positive Escherichia coli Strains

Salhi

Rabti

Dhehibi

et al. 2022

IJMS

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“…This gene was also employed for the development of an electrochemical sensor by Rabti et al (2020). The lacZ gene, coding for lactose permease, was employed to develop a sensor for the detection of E. coli in the work of Li et al (2015a).…”

Section: Stecmentioning

confidence: 99%

Electrochemical nucleic acid-based sensors for detection of E. coli and Shiga toxin-producing E. coli (STEC) – Review of the recent developments

Wasiewska

Seymour

Juska

et al. 2022

Preprint

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Escherichia coli (E. coli) are a group of bacteria, which are a natural part of the intestinal flora of warm-blooded animals, including humans. Most E. coli are non-pathogenic and are essential for the normal function of a healthy intestine, however, certain types such as Shiga toxin-producing E. coli (STEC) is a foodborne pathogen that can cause a life-threatening illness. Development of point-of-care (POC), rapid detection devices for E. coli have high importance from a food safety point of view. The most suitable way to distinguish between generic E. coli and STEC is by using nucleic acid-based detection, focusing on the virulence factors. Electrochemical sensors based on nucleic acid recognition have attracted much attention in recent years for use in pathogenic bacteria detection. This review has summarised nucleic acid-based sensors for the detection of generic E. coli and STEC since 2015. First, the sequences of the genes used as recognition probes are discussed and compared to the most recent research regarding the specific detection of general E. coli and STEC. Subsequently, the collected literature regarding nucleic acid-based sensors was described and discussed. The traditional sensors were divided into four categories such as gold, ITO, carbon-based electrodes, and those using magnetic particles. Finally, we summarised the future trends in nucleic acid-based sensor development for E. coli and STEC including some examples of fully integrated devices

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“…Electrochemical impedance spectroscopy (EIS) is widely used as the biosensing strategy for label-free electrochemical detection of formaldehyde, dibutyl phthalate, Escherichia coli, and cancer cells. [23][24][25][26] Since the phosphoric acid skeleton of DNA has dense negative charge, different kinds of reactions or conformational changes of DNA can be monitored by EIS. 27,28 Inspired by these concepts, we developed a label-free and ultrasensitive electrochemical impedance biosensor for ORAOV1 gene via Exo III-assisted target recycling, RecJf exonuclease-assisted background signal reduction, and terminal deoxynucleotide transferase (TdT)-mediated G-quadruplex amplification processes.…”

Section: Introductionmentioning

confidence: 99%

A label-free and ultrasensitive electrochemical biosensor for oral cancer overexpressed 1 gene via exonuclease III-assisted target recycling and dual enzyme-assisted signal amplification strategies

Zhang

Wang

Jin

et al. 2022

Analyst

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Impedimetric DNA E-biosensor for multiplexed sensing of Escherichia coli and its virulent f17 strains

Cited by 16 publications

References 32 publications

Sandwich-Based Immunosensor for Dual-Mode Detection of Pathogenic F17–Positive Escherichia coli Strains

Sandwich-Based Immunosensor for Dual-Mode Detection of Pathogenic F17–Positive Escherichia coli Strains

Electrochemical nucleic acid-based sensors for detection of E. coli and Shiga toxin-producing E. coli (STEC) – Review of the recent developments

A label-free and ultrasensitive electrochemical biosensor for oral cancer overexpressed 1 gene via exonuclease III-assisted target recycling and dual enzyme-assisted signal amplification strategies

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