Impedimetric evaluation of the efficiency of disinfectants against biofilms

Dhaliwal, D.S.; Cordier, J.-L.; Cox, L.J.

doi:10.1111/j.1472-765x.1992.tb00767.x

Cited by 38 publications

(19 citation statements)

References 8 publications

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“…Stainless steel surfaces are favourable to the formation of biofilm for Salmonella A particular difficulty with stainless steel, however, is that its surface can feature crevices and zones of corrosion, which can prevent an effective cell count. But more troubling is that these surface crevices can make it difficult to remove biofilms (Holah et al 1990;Dhaliwal et al 1992;Flint et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Biofilm-producing abilities of Salmonella strains isolated from Turkey

Karaca

Akçelik

2012

Biologia

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In the present study the biofilm-forming characteristics of 99 serotyped (DMC strains) and 41 genus levelidentified (IS strains) Salmonella strains originating from Turkey were investigated. The strains were selected based on their ability to show the biofilm morphotype on Congo red agar plates. In addition, all strains were evaluated with regard to properties related to forming pellicle structures, physical differences of pellicles, any changes in the media associated with the formation of pellicles, and the presence of cellulose within the formed biofilm matrix as determined using 366 nm UV light. The Salmonella Typhimurium DMC4 strain was the best producer of biofilm grown on polystyrene microtiter plates (optical density at 595 nm: 3.418). In subsequent experiments industrial process conditions were used to investigate different morphotyped Salmonella strains' biofilm-forming capability on stainless steel, a commonly preferred surface for the food industries, and on polystyrene surfaces. The effect of other important industrial conditions, such as temperature (5, 20, 37• C), pH (4.5, 5.5, 6.5, 7.4) and NaCl concentration (0.5, 1.5, 5.5, 10.5%) on the production of biofilm of the different morphotyped Salmonella strains (DMC4; red, dry and rough morphotyped S. Typhimurium, DMC12; brown, dry and rough morphotyped S. Infantis, DMC13; pink, dry and rough morphotyped S. subsp. Roughform) were also assessed. On the other hand, pH values exhibited variable effects on biofilm-forming features for different Salmonella strains on both polystyrene and stainless steel surfaces.

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Section: Discussionmentioning

confidence: 99%

Biofilm-producing abilities of Salmonella strains isolated from Turkey

Karaca

Akçelik

2012

Biologia

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“…The methods for prevention and elimination of biofilms include the use of antiadhesive coatings, antimicrobial agents, and biocides (4,8,10,23,27,35).…”

Section: Discussionmentioning

confidence: 99%

Use of a Quartz Crystal Microbalance To Investigate the Antiadhesive Potential of N -Acetyl- l -Cysteine

Olofsson

Hermansson

Elwing

2005

Appl Environ Microbiol

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The reduction of bacterial biofilm formation on stainless steel surfaces by N-acetyl-L-cysteine (NAC) is attributed to effects on bacterial growth and polysaccharide production, as well as an increase in the wettability of steel surfaces. In this report, we show that NAC-coated stainless steel and polystyrene surfaces affect both the initial adhesion of Bacillus cereus and Bacillus subtilis and the viscoelastic properties of the interaction between the adhered bacteria and the surface. A quartz crystal microbalance with dissipation was shown to be a powerful and sensitive technique for investigating changes in the applied NAC coating for initial cell surface interactions of bacteria. The kinetics of frequency and dissipation shifts were dependent on the bacteria, the life cycle stage of the bacteria, and the surface. We found that exponentially grown cells gave rise to a positive frequency shift as long as their cell surface hydrophobicity was zero. Furthermore, when the characteristics of binding between the cell and the surface for different growth phases were compared, the rigidity increased from exponentially grown cells to starved cells. There was a trend in which an increase in the viscoelastic properties of the interaction, caused by the NAC coating on stainless steel, resulted in a reduction in irreversibly adhered cells. Interestingly, for B. cereus that adhered to polystyrene, the viscoelastic properties decreased, while there was a reduction in adhered cells, regardless of the life cycle stage. Altogether, NAC coating on surfaces was often effective and could both decrease the initial adhesion and increase the detachment of adhered cells and spores. The most effective reduction was found for B. cereus spores, for which the decrease was caused by a combination of these two parameters.Bacteria commonly adhere to a surface and are part of a complex biofilm. Since the growth of biofilms causes enormous economic losses and health problems in industrial systems like food and paper production, huge efforts are made to solve this problem. The adhesion of bacteria to surfaces is a complex interplay of physical, chemical, and biological factors (5), and there are large variations in the adhesion behaviors of different bacteria and also for different life cycle stages of one strain (41). It is very important to consider these differences when antiadhesion coatings are developed. It is generally thought that bacterial adhesion is preceded by the adsorption of a conditioning layer of molecules that influence adhesion of bacteria to surfaces (43). A good antiadhesion coating should form a conditioning film that prevents adhesion and has a low cohesive strength, making a weak bond between the biofilm and the surface (6). One such agent may be N-acetyl-L-cysteine (NAC). NAC is commonly used in medical treatment of chronic bronchitis and cancer (38,46) and is one of the smallest drug molecules in use (30). Moreover, we recently demonstrated that NAC affects several processes that are important for bacterial biofilm fo...

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“…Some researchers have calculated the number of cells on stainless steel surfaces by relating the TTD to the equivalent number of cells, indicated by a standard calibration curve prepared from serial dilutions of planktonic cells [37,38]. Other studies of surface-inhabiting bacteria have also used calibration curves constructed from planktonic cell suspensions [39][40][41].…”

Section: Disinfection Efficacy Of Hssp Against E Coli Biofilm Estimmentioning

confidence: 99%

Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of <i>Escherichia coli</i> ATCC 25922 Surrogated for <i>E. coli</i> O157:H7

Kubo¹,

Ohshima²,

Irie³

et al. 2013

JBNB

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The ability of heated scallop-shell powder (HSSP) to disinfect Escherichia coli ATCC 25922 biofilm was investigated. On account of its cryotolerance and cell surface characteristics, the E. coli strain is reportedly a useful surrogate for E. coli O157: H7 in surface attachment studies. In this study, an E. coli ATCC 25922 biofilm was formed on a glass plate, and immersed in a slurry of HSSP. Following treatment, the disinfection ability of the HSSP toward the biofilm was non-destructively and quantitatively measured by conductimetric assay. The disinfection efficacy increased with HSSP concentration and treatment time. HSSP treatment (10 mg/mL, pH 12.5) for 20 min completely eliminated biofilm bioactivity (approximately 10 8 CFU/cm 2 in non-treated biofilms). In contrast, treatment with NaOH solution at the same pH, and treatment with sodium hypochlorite (200 g/mL) reduced the activity by approximately one to three log 10 . Fluorescence microscopy confirmed that no viable cells remained on the plate following HSSP treatment (10 mg/mL). Although alkaline and sodium hypochlorite treatments removed cells from the biofilm, under these treatments, many viable cells remained on the plate. To elucidate the mechanism of HSSP activity against E. coli ATCC 25922, the active oxygen generated from the HSSP slurry was examined by chemiluminescence analysis. The luminescence intensity increased with increasing concentration of HSSP slurry. The results suggested that, besides being alkaline, HSSP generates active oxygen species with sporicidal activity. Thus, HSSP treatment could also be effective for controlling biofilms of the toxic strain E. coli O157: H7, implicated in food poisoning.

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Impedimetric evaluation of the efficiency of disinfectants against biofilms

Cited by 38 publications

References 8 publications

Biofilm-producing abilities of Salmonella strains isolated from Turkey

Biofilm-producing abilities of Salmonella strains isolated from Turkey

Use of a Quartz Crystal Microbalance To Investigate the Antiadhesive Potential of N -Acetyl- l -Cysteine

Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of <i>Escherichia coli</i> ATCC 25922 Surrogated for <i>E. coli</i> O157:H7

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Impedimetric evaluation of the efficiency of disinfectants against biofilms

Cited by 38 publications

References 8 publications

Biofilm-producing abilities of Salmonella strains isolated from Turkey

Biofilm-producing abilities of Salmonella strains isolated from Turkey

Use of a Quartz Crystal Microbalance To Investigate the Antiadhesive Potential of N -Acetyl- l -Cysteine

Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of &lt;i&gt;Escherichia coli&lt;/i&gt; ATCC 25922 Surrogated for &lt;i&gt;E. coli&lt;/i&gt; O157:H7

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Disinfection Treatment of Heated Scallop-Shell Powder on Biofilm of <i>Escherichia coli</i> ATCC 25922 Surrogated for <i>E. coli</i> O157:H7