This review gives an overview on different types of affinity biosensors based on electropolymerized polymer films that are becoming an important class of analytical tools. These affinity biosensors may be classified according to the strategy used for their fabrication, namely entrapment within polymers during their electrochemical growth, simple adsorption onto electropolymerized films, chemical coupling or affinity interactions between bioreceptors and electropolymerized films or direct electrochemical polymerization of the bioreceptor itself. Recently opened perspectives and potential research directions are also discussed.