Imperatae rhizoma-Hedyotis diffusa Willd. herbal pair alleviates nephrotic syndrome by integrating anti-inflammatory and hypolipidaemic effects

Zou, Wei; Dong, Yongchun; Yang, Shicong; Gong, Linna; Zhang, Yan; Shi, Birui; La, Lei; Tang, Lan; Liu, Menghua

doi:10.1016/j.phymed.2021.153644

Cited by 10 publications

(4 citation statements)

References 29 publications

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“…Total RNA was extracted from the kidney tissue by the Trizol method following the kit instructions [41]. The kidney tissue was ground into a powder in liquid nitrogen, 1 mL of pre-cooled Trizol reagent was added, and then the tissue transferred to the centrifugal tube.…”

Section: Rt-qpcrmentioning

confidence: 99%

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Asperulosidic Acid Ameliorates Renal Interstitial Fibrosis via Removing Indoxyl Sulfate by Up-Regulating Organic Anion Transporters in a Unilateral Ureteral Obstruction Mice Model

Wang,

Shi,

Pan

et al. 2023

Molecules

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Asperulosidic acid is a bioactive iridoid isolated from Hedyotis diffusa Willd. with anti-inflammatory and renal protective effects. However, its mechanism on renal interstitial fibrosis has not been elucidated yet. The present study aims to explore whether asperulosidic acid could retard renal fibrosis by reducing the circulating indoxyl sulfate (IS), which is a uremic toxin and accelerates chronic kidney disease progression by inducing renal fibrosis. In this paper, a unilateral ureteral obstruction (UUO) model of Balb/C mice was established. After the mice were orally administered with asperulosidic acid (14 and 28 mg/kg) for two weeks, blood, liver and kidney were collected for biochemical, histological, qPCR and Western blot analyses. Asperulosidic acid administration markedly reduced the serum IS level and significantly alleviated the histological changes in glomerular sclerosis and renal interstitial fibrosis. It is noteworthy that the mRNA and protein levels of the organic anion transporter 1 (OAT1), OAT3 and hepatocyte nuclear factor 1α (HNF1α) in the kidney were significantly increased, while the mRNA expressions of cytochrome P450 2e1 (Cyp2e1) and sulfotransferase 1a1 (Sult1a1) in the liver were not altered after asperulosidic acid administration. These results reveal that asperulosidic acid could accelerate the renal excretion of IS by up-regulating OATs via HNF1α in UUO mice, thereby alleviating renal fibrosis, but did not significantly affect its production in the liver, which might provide important information for the development of asperulosidic acid.

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Section: Rt-qpcrmentioning

confidence: 99%

“…The total protein of renal tissue was extracted by Cell Signaling Technology [41]. The protein sample was added to the pre-cooled protein cracking buffer at the ratio of 1 mL/0.1 g, homogenized for 15 min, and centrifuged at 10,000 g/min for 15 min.…”

Section: Western Blottingmentioning

confidence: 99%

Asperulosidic Acid Ameliorates Renal Interstitial Fibrosis via Removing Indoxyl Sulfate by Up-Regulating Organic Anion Transporters in a Unilateral Ureteral Obstruction Mice Model

Wang,

Shi,

Pan

et al. 2023

Molecules

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“…This is characterized by the successive release of cytokines and chemokines in subjects with activated NF-κB signaling and the subsequent loss of serum proteins. 24…”

Section: Introductionmentioning

confidence: 99%

“…This is characterized by the successive release of cytokines and chemokines in subjects with activated NF-κB signaling and the subsequent loss of serum proteins. 24 Electrolyte abnormalities and edema in NS mice are associated with activated Renin-Angiotensin-Aldosterone System (RAAS), which is attributed to hypo albuminaemia-induced inadequacy of effective blood volume. 25,26 In addition, lipid dys-homeostasis is a cardinal feature seen in many patients with NS, and leads to serious nephrotoxicity which includes podocyte injury, glomerular basement membrane (GBM) dysfunction, and inflammation.…”

Section: Introductionmentioning

confidence: 99%

Beneficial effects of procyanidin B2 on adriamycin-induced nephrotic syndrome mice: the multi-action mechanism for ameliorating glomerular permselectivity injury

Zhao

Tang

et al. 2022

Food Funct.

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Molecular signaling pathways in doxorubicin-induced nephrotoxicity and potential therapeutic agents

Lu,

Wei,

Gao

et al. 2025

International Immunopharmacology

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Imperatae rhizoma-Hedyotis diffusa Willd. herbal pair alleviates nephrotic syndrome by integrating anti-inflammatory and hypolipidaemic effects

Cited by 10 publications

References 29 publications

Asperulosidic Acid Ameliorates Renal Interstitial Fibrosis via Removing Indoxyl Sulfate by Up-Regulating Organic Anion Transporters in a Unilateral Ureteral Obstruction Mice Model

Asperulosidic Acid Ameliorates Renal Interstitial Fibrosis via Removing Indoxyl Sulfate by Up-Regulating Organic Anion Transporters in a Unilateral Ureteral Obstruction Mice Model

Beneficial effects of procyanidin B2 on adriamycin-induced nephrotic syndrome mice: the multi-action mechanism for ameliorating glomerular permselectivity injury

Molecular signaling pathways in doxorubicin-induced nephrotoxicity and potential therapeutic agents

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