Implantation and Establishment of Pregnancy in Ruminants

Spencer, Thomas E.; Hansen, Thomas

doi:10.1007/978-3-319-15856-3_7

Cited by 91 publications

(81 citation statements)

References 202 publications

(283 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results found that dynamic changes in the ULF secretome do not occur in association with the development of uterine receptivity. This finding starkly contrasts with analysis of the ULF secretome during early pregnancy in large animals such as cattle, sheep and pigs226162. Results of the present study to support the idea that uterine GE have a primarily biological role in homeostasis of the ULF, because many proteins and most amino acids were more abundant in ULF from PUGKO than WT mice.…”

Section: Discussioncontrasting

confidence: 77%

Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation

Kelleher

Burns

Behura

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

Uterine glands are essential for pregnancy in mice and likely humans, because they secrete or transport bioactive substances that regulate uterine receptivity for blastocyst implantation. In mice, the uterus becomes receptive to blastocyst implantation on day 4, but is refractory by day 5. Here, blastocysts could be recovered from progesterone-induced uterine gland (PUGKO) but not wildtype (WT) mice on day 5 post-mating. Anti-adhesive Muc1 protein and microvilli were present on the luminal epithelium of PUGKO but not WT uteri. A number of known uterine receptivity genes and gland-specific genes were altered in the PUGKO uterus. Next, the uterus and uterine luminal fluid (ULF) were obtained from WT and PUGKO mice on day 3, 4 and 5. Transcriptome analysis revealed that 580 genes were decreased in the PUGKO uterus, however ULF secrotome analysis revealed that many proteins and several amino acids were increased in the PUGKO ULF. Of note, many proteins encoded by many gland-specific genes were not identified in the ULF of WT mice. These results support the ideas that uterine glands secrete factors that regulate ULF homeostasis and interact with other cell types in the uterus to influence uterine receptivity and blastocyst implantation for the establishment of pregnancy.

show abstract

Section: Discussioncontrasting

confidence: 77%

Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation

Kelleher

Burns

Behura

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…2 and is shown in greater detail in other reviews (McCracken et al, 1999;Spencer and Hansen, 2015). Clearly the induction of E2 receptors has a central role in the mechanisms that initiate these pulses.…”

Section: Period Ii: Normal Luteolysis: Role Of Uterus and Pgf -Days 1mentioning

confidence: 99%

Maintenance or regression of the corpus luteum during multiple decisive periods of bovinepregnancy

Wiltbank¹,

Meidan²,

Ochoa³

et al. 2016

Anim Reprod

View full text Add to dashboard Cite

In ruminants, there are specific times during the estrous cycle or pregnancy when the corpus luteum (CL) may undergo regression. This review has attempted to summarize the physiological and cellular mechanisms involved in CL regression or maintenance during four distinct periods. The first period is near day 7 when animals that are ovulating after a period of low circulating progesterone (P4), such as first pubertal ovulation or first postpartum ovulation, are at risk of having a premature increase in Prostaglandin F2α (PGF) secreted from the uterus resulting in early CL regression and a short estrous cycle. The second period is when normal luteolysis occurs at day 18-25 of the cycle or when the CL is rescued by interferon-tau secreted by the elongating embryo. The uterine mechanisms that determine the timing of this luteolysis or the prevention of luteolysis have been generally defined. Induction and activation of endometrial E2 receptors result in induction of endometrial oxytocin receptors that can now be activated by normal pulses of oxytocin. Of particular importance is the observation that the primary mechanisms are only activated through local (ipsilateral) and not a systemic route due to transfer of PGF from the uterine vein to the ovarian artery. In addition at the CL level, studies are providing definition to the cellular and molecular mechanisms that are activated in response to uterine PGF pulses or pregnancy. The third period that is discussed occurs in the second month of pregnancy (day 28-60) when undefined mechanisms result in CL maintenance of an ipsilateral CL but regression of a contralateral (opposite side from pregnancy) CL. The final period that is discussed is regression of the CL just prior to parturition. Although, cortisol from the fetus appears to be the primary initiator of luteolysis, PGF seems to be the final signal that causes regression of the CL. Thus, in all four periods, regression of the CL is likely to be caused by the direct actions of PGF that is secreted from the uterus. The uterine mechanisms that result in secretion of PGF seem to be normally inhibited during the early luteal phase, making short luteal phases not a normal event, and are altered during early pregnancy (day 18-25) resulting in prevention of luteolysis. During much of pregnancy, the mechanisms that cause PGF secretion from the uterus in response to oxytocin are intact but luteolysis does not normally occur, perhaps due to lack of efficient utero-ovarian transfer of PGF.

show abstract

“…Many researchers reported that the IFNT exists its effect on blood cells [7,[16][17][18] , CL [6,[19][20][21] , and liver [9] in the ovine and bovine. It is through paracrine and endocrine style that IFNT secreted by the conceptus releases into the uterine vein, and extends the luteal life span during early pregnancy in sheep [8,22,23] . Therefore, it is possible that IFNT exerts its effect on the BM, which is implicated in regulating the maternal immune system through blood circulation during early pregnancy in sheep.…”

Section: Discussionmentioning

confidence: 99%

Koyunların Erken Gebelik Döneminde Kemik iliğinde ISG15 Ekspresyonu

Yang¹,

Liu²,

Yan³

et al. 2017

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Interferon-tau (IFNT) is the main signal for maternal recognition of pregnancy in ruminants. IFNT acts on the endometrium, corpus luteum, and liver through paracrine and endocrine style, which is involved in inhibiting the development of luteolytic mechanism and suppressing maternal immune rejection of the semi-allogeneic fetus. Bone marrow (BM) is a key component of the lymphatic system, supports the body's immune system through producing the lymphocytes. At present study, the BM was obtained from days 13, 16 and 25 of pregnant ewes, day 16 of non-pregnant ewes to study the expression of interferon stimulated gene 15 kDa protein (ISG15) mRNA and protein through a qRT-PCR assay, Western blot, and an immunohistochemistry analysis. Our results showed that the expression of ISG15 mRNA, proteins and conjugated proteins were up-regulated in the stroma of BM during early pregnancy, and the immunohistochemistry results confirmed that the ISG15 proteins were localized in the cytoplasm of different cells in the stroma of BM. In conclusion, IFNT derived from the conceptus induced upregulated expression of ISG15 and conjugated proteins in the stroma of BM through an endocrine style, which were involved in regulating the maternal immune response during early pregnancy in ewes. Keywords: Ewes, Bone marrow, Pregnancy, Interferon stimulated gene 15 kDa protein Koyunların Erken Gebelik Döneminde Kemik İliğinde ISG15 Ekspresyonu Özetİnterferon-tau (IFNT) ruminantlarda gebeliğin maternal tanısındaki başlıca sinyaldir. IFNT endometrium, korpus luteum ve karaciğer üzerinde parakrin ve endokrin yollarla etki ederek luteolitik mekanizmanın gelişmesini inhibe eder ve semi-allojenik fetusun anne tarafından reddini baskılar. Kemik iliği lenfatik sistemin en önemli bileşenlerinden biri olup lenfositleri üretmek suretiyle vücudun bağışıklık sistemini desteklemektedir. Bu çalışmada, 13, 16 ve 25. günlerde gebe koyunlardan ve 16. günde gebe olmayan koyunlardan kemik iliği alınarak interferon tarafından uyarılan gen 15 kDa protein (ISG15) mRNA ve protein ekspresyonu qRT-PCR, Western blot ve immunohistokimyasal yöntemlerle araştırıldı. Elde edilen sonuçlar ISG15 mRNA, proteinler ile konjuge proteinlerin ekspresyonunun erken gebelik döneminde kemik iliğinde upregule edildiğini ve immunohistokimyasal olarak ISG15 proteinlerin kemik iliğinin stromasında farklı hücrelerin sitoplazmasında yer aldığını göstermiştir. Sonuç olarak, gebeliğe bağlı olarak üretilen IFNT; ISG15 ve konjuge proteinlerin ekspresyonunu kemik iliğinin stromasında endokrin yolla upregüle etmiş ve böylece koyunların erken gebelik döneminde maternal bağışıklık cevabın düzenlenmesinde rol almıştır.

show abstract

Implantation and Establishment of Pregnancy in Ruminants

Cited by 91 publications

References 202 publications

Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation

Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation

Maintenance or regression of the corpus luteum during multiple decisive periods of bovinepregnancy

Koyunların Erken Gebelik Döneminde Kemik iliğinde ISG15 Ekspresyonu

Contact Info

Product

Resources

About